Development of neurotoxicity risk assessment of environmental pollutant for young children

• Project/Area Number: 20760361
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Kyoto University
• Principal Investigator: NAKAYAMA Aki Kyoto University, 大学院・工学研究科, 准教授 (10335200)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 幼児 / 鉛 / 神経毒性 / 神経細胞 / タンパク質発現変動 / HO-2 / 血液脳関門 / こども / 幼若ラット / 血中鉛 / 脳中鉛 / 神経細胞曝露レベル / heme oxygenase2 / PC12細胞 / バイオマーカー / タンパク質発現変動解析

Research Abstract

The concentrations of Pb in blood and brain following oral doses were examined and it was found that the both concentrations were almost the same. Neuronal model cells, PC12, were exposed to lead acetate, and he protein expression patterns were examined. It was found that the expression of HO-2(heme oxygenase 2) was decreased at the concentration more then 0.5 microM. The expression of HO-2 is possibly more sensitive biomarker to Pb and could be useful for risk assessment.

Research Products

• [Journal Article] Changes in Expression Level of Heme Oxygenase-2 in Lead- Exposed Neuronal PC-12 Cells (2010)
  • Author(s): Cesar Ortinero, Aki Nakayama, Chiharu Tomomatsu, Katsuya Okawa, Shinsuke Morisawa, Minoru Yoneda
  • Journal Title: International Journal of Arts and Sciences Pages: 1-12
  • Peer Reviewed
• [Journal Article] 神経影響に着目した鉛化合物のヒト健康リスク評価に関する研究 (2010)
  • Author(s): 谷修祐, 中山亜紀, 米田稔, 森澤眞輔
  • Journal Title: 日本リスク研究学会研究発表会講演論文集, 日本リスク研究学会研究発表会講演論文集 vol.22 Pages: 61-66
• [Journal Article] 神経毒性に着目した鉛のヒト健康リスク評価に関する研究 (2010)
  • Author(s): 谷修祐, 中山亜紀, 米田稔
  • Journal Title: 環境衛生工学研究, 環境衛生工学研究, 環境衛生工学研究 vol.24 (3) Pages: 78-81
• [Journal Article] 神経毒性に着目した鉛のヒト健康リスク評価に関する研究 (2010)
  • Author(s): 谷修祐, 中山亜紀, 米田稔
  • Journal Title: 環境衛生工学研究 Volume: 24(3) Pages: 78-81
• [Journal Article] Changes in expression level of heme oxygenase-2 in lead-exposed neuronal PC-12 cells. (2010)
  • Author(s): Ortinero, C., Nakayama, A., Tomomatsu, C., Okawa, K., Morisawa, S., Yoneda, M.
  • Journal Title: International Journal of Arts and Sciences Volume: 3(9) Pages: 58-69
• [Journal Article] 神経影響に着目した鉛化合物のヒト健康 (2009)
  • Author(s): 谷修祐, 中山亜紀, 米田稔, 森澤眞輔
  • Journal Title: 日本リスク研究学会第22回年次大会講演論文集 Pages: 61-66
  • Peer Reviewed
• [Presentation] Expression of Ras-related Rab-1B protein in lead-treated neuronal PC-12 cells) (2011)
  • Author(s): Ortinero, C., Nakayama, A., Tomomatsu, C., Okawa, K., Morisawa, S., Yoneda, M.
  • Organizer: 6th Asian Pacific Organization for Cell Biology Congress
  • Place of Presentation: Manila, Philippines
  • Year and Date: 2011-02-25
• [Presentation] 神経影響に着目した鉛化合物のヒト健康リスク評価に関する研究 (2010)
  • Author(s): 谷修祐, 中山亜紀, 米田稔, 森澤眞輔
  • Organizer: 日本リスク研究学会研究発表会講演論文集, vol.22 pp.61-66
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 神経毒性に着目した鉛のヒト健康リスク評価に関する研究 (2010)
  • Author(s): 谷修祐, 中山亜紀, 米田稔
  • Organizer: 環境衛生工学研究, 環境衛生工学研究vol.24 (3) pp.78-81
  • Place of Presentation: 京都
  • Year and Date: 2010-07-31
• [Presentation] Changes in expression level of heme oxygenase-2 in lead-exposed neuronal PC-12 cells (2010)
  • Author(s): Cesar Ortinero, Aki Nakayama, Chiharu Tomomatsu, Katsuya Ohkawa, Minoru Yoneda, Shinsuke Morisawa
  • Organizer: International Journal of Arts & Sciences Mediterranean Conference
  • Place of Presentation: Gozo, Malta
  • Year and Date: 2010-02-15
• [Presentation] Expression of Ras-related Rab-1B protein in lead-treated neuronal PC-12 cells (2010)
  • Author(s): Cesar Ortinero, Aki Nakayama, Shinsuke Morisawa, Minoru Yoneda
  • Organizer: 6th Asian Pacific Organization for Cell Biology Congress
  • Place of Presentation: Manila, Philippines
• [Presentation] Exposure of preschool children to lead in playgrounds in Metro Manila, Philippines (2010)
  • Author(s): Cesar Ortinero, Maiko Ikegami, Aki Nakayama, Shinsuke Morisawa, Minoru Yoneda
  • Organizer: International Conference on the Environment and Natural Resources
  • Place of Presentation: Mahidol University, Salaya, Thailand
• [Presentation] Changes in expression of heme oxygenase-2 in lead-exposed neuronal PC-12 cells Mediterranean Conference for Academic Disciplines of the International Journal of Arts and Sciences (2010)
  • Author(s): Cesar Ortinero, Aki Nakayama, Shinsuke Morisawa, Minoru Yoneda
  • Place of Presentation: University of Malta Gozo Campus, Malta
• [Presentation] 神経系培養細胞PC12における酢酸鉛のタンパク質発現変動 (2008)
  • Author(s): 中山亜紀, 友松千晴, 谷修介, 森澤眞輔, 大川克也, 米田稔
  • Organizer: 第35回日本トキシコロジー学会学術年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-27