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Development of bio-sensing element utilizing engineered biologically active peptides

Research Project

Project/Area Number 20760543
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Technology

Principal Investigator

MIYACHI Hirotaka  Tokyo University of Technology, 応用生物学部, 助教 (50358131)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsバイオセンサー / バイオテクノロジー / 生体機能利用 / 蛋白質
Research Abstract

Despite a variety of alternative strategies for protein and peptide immobilization, it remains a central challenge to develop general techniques to immobilize functional peptides onto solid supports. In this study, the high density immobilization method of the peptide chain for the detection of a target molecule has been examined.
The molecular recognition peptide was designed and tested as a hiosensor element. The peptides designed in this study were analyzed by surface plasmon resonance (SPR) sensor.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (8 results)

All 2010 2009 2008

All Presentation (7 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] ロイシンジッパーモチーフの特性を利用したペプチドの高密度固定化法の開発2010

    • Author(s)
      佐々木達也, 宮地寛登
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東大・駒場
    • Year and Date
      2010-03-29
    • Related Report
      2009 Final Research Report
  • [Presentation] ロイシンジッパーモチーフの特性を利用したペプチドの高密度固定化法の開発2010

    • Author(s)
      佐々木達也、宮地寛登
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東大(駒場)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 分子認識ペプチドの設計および高密度化法の開発2009

    • Author(s)
      佐々木達也、宮地寛登
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ペプチドの分子認識能を用いた血管増殖因子(VEGF)検出法の開発2009

    • Author(s)
      原田将吾、宮地寛登
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 蛍光標識分子認識ペプチドを用いた血管増殖因子(VEGF)検出法の開発2009

    • Author(s)
      高木政人、宮地寛登
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 標的タンパク質を認識するペプチド鎖の高密度固定化法の開発2009

    • Author(s)
      宮地寛登、軽部征夫
    • Organizer
      日本農芸化学会2009年度大会[福岡]
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 標的タンパク質を認識するペプチド鎖の設計および固定化法の開発2008

    • Author(s)
      宮地寛登、宮本潔子、松久保悠、軽部征夫
    • Organizer
      標的タンパク質を認識するペプチド鎖の設計および固定化法の開発
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 高感度な生体物質固定化方法2008

    • Inventor(s)
      宮地寛登外川剛
    • Industrial Property Rights Holder
      オリンパス(株
    • Industrial Property Number
      2008-255860
    • Filing Date
      2008-09-30
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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