Functional analysis of potassium transporters interacting with SnRK2 protein kinases in Arabidopsis.

• Project/Area Number: 20770042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: UMEZAWA Taishi The Institute of Physical and Chemical Research, 機能開発研究チーム, 研究員 (70342756)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: プロテインキナーゼ / カリウムトランスポーター / シロイヌナズナ / リン酸化 / 環境ストレス / アブシジン酸 / シグナル伝達 / SnRK2プロテインキナーゼ / アプシジン酸

Research Abstract

SnRK2 family act as an important signaling factor for stress responses in plant cells. Here, we screened interacting partners of SnRK2 by yeast two-hybrid analysis, and isolated two potassium transporter-like proteins. To investigate their functions, we established Arabidopsis knockout mutants and checked any phenotypic changes in the mutants. These mutants showed a significant changes in potassium uptake under salt stress. Our results suggested SnRK2 and potassium transporters cooperatively functions in stress responses in root tissues.

Research Products

• [Journal Article] The PP2C-SnRK2 complex: The central regulator of an abscisic acid signaling pathway. (2010)
  • Author(s): Hirayama, T., Umezawa, T.
  • Journal Title: Plant Signal. Behav. 5(2) Pages: 160-163
  • Peer Reviewed
• [Journal Article] The PP2C-SnRK2 complex : The central regulator of an abscisic acid signaling pathway (2010)
  • Author(s): Hiryama T., Umezawa T.
  • Journal Title: Plant Signaling & Behavior 5(2)(オンライン)
  • Peer Reviewed
• [Journal Article] Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. (2009)
  • Author(s): Fujita, Y., Nakashima, K., Yoshida, T., Katagiri, T., Kidokoro, S., Kanamori, N., Umezawa, T., Fujita, M., Maruyama, K., Ishiyama, K., Kobayashi, M., Nakasone, S., Yamada, K., Ito, T., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Plant Cell Physiol. 50(12) Pages: 2123-2132
  • NAID: 10027345131
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SRK2E/OST1/ SnRK2. 6 protein kinase. (2009)
  • Author(s): Sato, A., Sato, Y., Fukao, Y., Fujiwara, M., Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M., Miyake, H., Goto, D.B., Uozumi, N.
  • Journal Title: Biochem. J. 424(3) Pages: 439-448
  • Peer Reviewed
• [Journal Article] Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. (2009)
  • Author(s): Umezawa, T., Sugiyama, Y., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Shinozaki, K.
  • Journal Title: Proc. Nat. Acad. Sci. USA 106(41) Pages: 17588-93
  • Peer Reviewed
• [Journal Article] Three Arabidopsis SnRK2 protein kinases, SRK2D/ SnRK2. 2, SRK2E/SnRK2. 6/OST1 and SRK2I/ SnRK2. 3 involved in ABA-signaling are essential for the control of seed development and dormancy. (2009)
  • Author(s): Nakashima, K., Fujita, Y., Kanamori, N., Katagiri, T., Umezawa, T., Kidokoro, S., Maruyama, K., Yoshida, T., Ishiyama, K., Kobayashi, M., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Plant Cell Physiol. 50(7) Pages: 1345-63
  • Peer Reviewed
• [Journal Article] Three SnRK2 protein kinases are the main positive regulators of a bscisic acid signaling in response to water stress in Arabidopsis (2009)
  • Author(s): Fujita, Y., Nakashima, K., Yoshida, T., Katagiri, T., Kidokoro, S., Kanamori, N., Umezawa, T., Fujita, M., Maruyama, K., Ishiyama, K., Kobayashi, M., Nakasone, S., Yamada, K., Ito, T., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Plant, Cell & Physiology 50 Pages: 2123-2132
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SRK2E/OST1/SnRK2.6 protein kinase (2009)
  • Author(s): Sato, A., Sato, Y., Fukao, Y., Fujiwara, M., Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M., Miyake, H., Goto, D.B., Uozumi, N.
  • Journal Title: Biochemical Journal 424 Pages: 439-448
  • Peer Reviewed
• [Journal Article] Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis (2009)
  • Author(s): Umezawa, T., Sugiyama, Y, Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Shinozaki, K.
  • Journal Title: Proceedings of the National Academy of Sciences in the United States of America 106 Pages: 17588-17593
  • Peer Reviewed
• [Journal Article] Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3 involved in ABA-signaling are essential for the control of seed development and dormancy (2009)
  • Author(s): Nakashima, K., Fujita, Y., Kanamori, N., Katagiri, T., Umezawa, T., Kidokoro, S., Maruyama, K., Yoshida, T., Ishiyama, K., Kobayashi, M., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Plant, Cell & Physiology 50 Pages: 1345-1363
  • NAID: 10027342032
  • Peer Reviewed
• [Journal Article] Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought- inducible gene expression.
  • Author(s): *Mizoguchi, M., *Umezawa, T., Nakashima, K., Kidokoro, S., Takasaki, H., Fujira, Y., Yamaguchi-Shinozaki, K., Shinozaki, K.
  • Journal Title: Plant Cell Physiol. (in press)
  • NAID: 10027349490
  • Peer Reviewed
• [Presentation] タンパク質のリン酸化・脱リン酸化が支配する植物ホルモンアブシジン酸のシグナル伝達経路 (2010)
  • Author(s): 梅澤泰史・平山隆志・篠崎一雄
  • Organizer: 第51回日本植物生理学会年会シンポジウム7「タンパク質の翻訳後修飾と植物の機能制御」
  • Place of Presentation: 熊本(口頭発表)
  • Year and Date: 2010-03-22
• [Presentation] アブシジン酸ジグナル伝達におけるSnRK2プロテインキナーゼとPP2Cプロテインホスファターゼの相互作用 (2010)
  • Author(s): 梅澤泰史・溝口昌秀・林晋平・平山隆志・篠崎一雄
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-19
• [Presentation] The PP2C-SnRK2 complex: a central regulatory module in abscisic acid signaling. (2010)
  • Author(s): Umezawa, T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T., Shinozaki, K.
  • Organizer: Plant Protein Phosphorylation Symposium, University of Missouri-Columbia
  • Place of Presentation: Columbia, MO(口頭発表)
• [Presentation] アブシジン酸シグナル伝達におけるプロテインキナーゼとプロテインホスファターゼの相互作用 (2009)
  • Author(s): 梅澤泰史
  • Organizer: 島根大学細胞工学セミナー
  • Place of Presentation: 島根大学
  • Year and Date: 2009-12-18
• [Presentation] アブシジン酸シグナル伝達におけるプロテインキナーゼとプロテインホスファターゼの相互作用 (2009)
  • Author(s): 梅澤泰史
  • Organizer: 農業生物資源研究所QTLゲノム育種研究センターセミナー
  • Place of Presentation: 農業生物資源研究所
  • Year and Date: 2009-12-04
• [Presentation] Type 2C Protein Phophatases Directly Regulate Abscisic Acid-Activated protein Kinases in Arabidopsis. (2009)
  • Author(s): Umezawa T.
  • Organizer: North Carolina Plant Molecular Biology Consortium Seminar Series, North Carolina Biotechnology Center
  • Place of Presentation: Research Triangle Park, NC(口頭発表)
  • Year and Date: 2009-11-02
• [Presentation] Type 2C Protein Phophatases Directly Regulate Abscisic Acid-Activated protein Kinases in Arabidopsis (2009)
  • Author(s): 梅澤泰史
  • Organizer: North Carolina Plant Molecular Biology Consortium Seminar Series
  • Place of Presentation: North Carolina Biotechnology Center
  • Year and Date: 2009-11-02
• [Presentation] Physical and functional interactions between 2C-type protein phosphatases and SnRK2 protein kinases in ABA signaling (2009)
  • Author(s): Umezawa, T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T., Shinozaki, K.
  • Organizer: The 9th International Plant Molecular Biology Congress
  • Place of Presentation: St.Louis, MO
  • Year and Date: 2009-10-25
• [Presentation] Physical and functional interactions between 2C-type protein phosphatases and SnRK2 protein kinases in ABA signaling. (2009)
  • Author(s): Umezawa, T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T., Shinozaki, K.
  • Organizer: The 9th International Plant Molecular Biology Congress
  • Place of Presentation: St. Louis, MO(ポスター発表)
• [Presentation] A Large-scale Analysis of Phosphorylated Proteins Regulated by ABA or Drought Stress. (2009)
  • Author(s): Umezawa, T., Sugiyama, N., Mizoguchi, M., Anderson, J.C., Peck, S.C., Ishihama, Y., Shinozaki, K.
  • Organizer: International Conference Plant Abiotic Stress Tolerance
  • Place of Presentation: Vienna, Austria(ポスター発表)
• [Remarks] プレスリリース(2009年9月22日)劣悪環境に応答する植物ホルモン「アブシジン酸」の応答経路を解明
  • URL: http://www.riken.jp/r-world/info/release/press/2009/090922/detail.html
• [Remarks] 平成21年9月22日付プレスリリース「劣悪環境に応答する植物ホルモン「アブシジン酸」の応答経路を解明」
  • URL: http://www.riken.go.jp/r-world/info/release/press/2009/090922/detail.html