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Structural analysis of cytoskeletal regulatory protein

Research Project

Project/Area Number 20770093
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SUETSUGU Kyoko  The Institute of Physical and Chemical Research, システム研究チーム, 研究員 (40391990)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞骨格 / X線構造解析 / 細胞膜 / タンパク質 / シグナル伝達 / 低分子Gタンパク質 / アクチン / X線結晶構造解析 / 細胞運動
Research Abstract

We solved the crystal structures of N-WASP binding protein ; EFCLpacsin2 (residues 1-343 ; EFC/F-BAR domain + C-terminal extension) and EFCSpacsin2 (residues1-305 ; EFC/F-BAR domain) at 2.7 and 2.0 A resolutions, respectively. We found that overexpression of the pacsin2 EFC/F-BAR domain induced cellular microspikes, with the pacsin2 EFC/F-BAR domain concentrated at the neck. The hydrophobic loops and the basic amino-acid residues on the concave surface of the pacsin2 EFC/F-BAR domain are essential for both the microspike formation and tubulation. Since the curvature of the neck of the microspike and that of the tubulation share similar geometry, the pacsin2 EFC/FBAR domain is considered to facilitate both microspike formation and tubulation.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (3 results)

All 2010 2008 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results)

  • [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II.2010

    • Author(s)
      Shimada A, Takano K, Shirouzu M, Hanawa-Suetsugu K, Terada T, Toyooka K, Umehara T, Yamamoto M, Yokoyama S
    • Journal Title

      Suetsugu S. FEBS Lett 19,584(6)

      Pages: 1111-8

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases.2008

    • Author(s)
      Kukimoto-Niino M, Sakamoto A, Kanno E, Hanawa-Suetsugu K, Terada T, Shirouzu M, Fukuda M, Yokoyama S.
    • Journal Title

      Structure 16

      Pages: 1478-1490

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II

    • Author(s)
      Atsushi Shimada, et al.
    • Journal Title

      FEBS Letters (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed

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Published: 2008-04-01   Modified: 2016-04-21  

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