Structural and functional roles of Ca2+ in the light-harvesting 1 complex from thermophilic purple sulfur bacterium Thermochromatium tepidum

• Project/Area Number: 20770102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Kobe University
• Principal Investigator: KIMURA Yukihiro Kobe University, 自然科学系先端融合研究環重点研究部, 助教 (20321755)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 紅色光合成細菌 / 耐熱性 / カルシウム / ストロンチウム / 金属蛋白質 / 光捕集反応中心複合体 / カルシウムイオン / ストロンチウムイオン / 生物物理 / 蛋白質 / 光合成

Research Abstract

Ca ions are responsible for unique properties of the purple sulfur photosynthetic bacterium Thermochromatium tepidum which is a moderate thermophile grown at the highest temperature among purple bacteria and shows an unusual LH1 Q_y absorption. The present spectroscopic and thermodynamic analyses revealed that the Ca^<2+>-binding little affects secondary structures of the LH1 proteins but precisely modulates conformation of BChl a and thermal stability of the LH1-RC complex. Furthermore, we discovered that Sr ion is an alternative cofactor biofunctionally replaceable with Ca ion in this thermophilic organism.

Research Products

• [Journal Article] A Spectroscopic Variant of the Light-Harvesting 1 Core Complex from the Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum (2011)
  • Author(s): Kimura, Y., Inada, Y., Yu, L.-J., Wang, Z.-Y., Ohno, T.
  • Journal Title: Biochemistry 50 Pages: 3638-3648
  • Peer Reviewed
• [Journal Article] A Spectroscopic Variant of the Light-Harvesting 1 Core Complex from the Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum (2011)
  • Author(s): Y.Kimura
  • Journal Title: Biochemistry Volume: (in press)
  • Peer Reviewed
• [Journal Article] Strontium Ions Are Functionally Replaceable with Calcium Ions in the Light-Harvesting 1 Reaction Center Core Complex from Thermophilic Purple Sulfur bacterium Thermochromatium tepidum (2011)
  • Author(s): Y.Kimura
  • Journal Title: Proceedings of 15^<th> International Congress on Photosynthesis Volume: (in press)
• [Journal Article] Calcium Ions Are Involved in the Thermostability and the Unusual Red-Shift of LH1 Q_y Transition of the Core Complex from Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum (2011)
  • Author(s): Y.Kimura
  • Journal Title: Proceedings of 15^<th> International Congress on Photosynthesis Volume: (in press)
• [Journal Article] Calcium Ions Are Required for the Enhanced Thermal Stability of the Light-harvesting-Reaction Center Core Complex from Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum (2009)
  • Author(s): Kimura, Y., Yu, L.-J., Hirano, Y., Suzuki, H., Wang, Z.-Y.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 93-99
  • Peer Reviewed
• [Journal Article] Strontium ions are functionally replaceable with calcium ions in the light-harvesting 1 reaction center core complex from Thermophilic purple sulfur bacterium Thermochromatium tepidum
  • Author(s): Kimura, Y., Inada, Y. Yu, L.-J., Ohno, T., Wang, Z.-Y.
  • Journal Title: proceedings of 15^<th> International Congress on Photosynthesis, Beijing, Aug. in press
• [Journal Article] Calcium ions are involved in the thermostability and the unusual red-shift of LH1 Q transition of the core complex from Thermophilic purple sulfur bacterium Thermochromatium tepidum
  • Author(s): Kimura, Y., Yu, L.-J., Hirano, Y., Suzuki, H., Wang, Z.-Y.
  • Journal Title: proceedings of 15^<th> International Congress on Photosynthesis, Beijing, Aug. in press
• [Presentation] Structural and functional roles of metal cations in the Photosynthetic core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum (2010)
  • Author(s): Kimura, Y., Inada, Y., Hirano, Y., Yu, L.-J., Wang, Z.-Y, Ohno, T.
  • Organizer: The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, U.S.A
• [Presentation] Strontium Ions Are Functionally Replaceable with Calcium Ions in the Light-Harvesting 1 Reaction Center Core Complex from Thermophilic Purple Sulfur bacterium Thermochromatium tepidum (2010)
  • Author(s): Kimura, Y., Inada, Y., Yu, L.-J., Ohno, T., Wang, Z.-Y.
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: Beijing, China, Aug
• [Presentation] Calcium Ions Are Involved in the Thermostability and the Unusual Red-Shift of LH1 Q Transition of the Core Complex from Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum. (2010)
  • Author(s): Kimura, Y., Yu, L.-J., Hirano, Y, Suzuki, H., Wang, Z.-Y.
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: Beijing, China
• [Presentation] 生合成的にSr^<2+>置換された好熱性紅色硫黄細菌Thermochromatium tepidumにおける色素蛋白質複合体の特性評価 (2010)
  • Author(s): 木村行宏、稲田勇太、春日草千子、佐々木悠子、Long-jiang Yu、Zheng-Yu Wang、大野隆
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
• [Presentation] Structural and functional roles of metal cations in the Photosynthetic core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum (2010)
  • Author(s): Y.Kimura
  • Organizer: The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu (USA)
• [Presentation] Strontium Ions Are Functionally Replaceable with Calcium Ions in the Light-Harvesting 1 Reaction Center Core Complex from Thermophilic Purple Sulfur bacterium Thermochromatium tepidum (2010)
  • Author(s): Y.Kimura
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: Beijing (China)
• [Presentation] Calcium Ions Are Involved in the Thermostability and the Unusual Red-Shift of LH1 Q_y Transition of the Core Complex from Thermophilic Purple Sulfur Bacterium Thermochromatium tepidum (2010)
  • Author(s): Y.Kimura
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: Beijing (China)
• [Presentation] 生合成的にSr^<2+>置換された好熱性紅色硫黄細菌Thermochromatium tepidumにおける色素蛋白質複合体の特性評価 (2010)
  • Author(s): 木村行宏
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] 好熱性紅色光合成細菌由来Cytochromec'の耐熱性に関する研究 (2009)
  • Author(s): 木村行宏
  • Organizer: 特定領城研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] 光合成光捕集反応中心複合体におけるCa^<2+>結合部位の検証 (2009)
  • Author(s): Zheng-YuWang
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] 好熱性光合成細菌Thermochromatium tepidum由来光捕集タンパク質-反応中心複合体の構造解析 (2009)
  • Author(s): 平野優
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] 好熱性紅色光合成細菌由来Cytochrome c'の耐熱性に関する研究 (2009)
  • Author(s): 木村行宏、平野優、鈴木秀明、佐々木悠子、大野隆、三木邦夫、Zheng-Yu Wang.
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 豊中
• [Presentation] 好熱性光合成細菌Thermochromatium tepudum由来光捕集タンパク質.反応中心複合体の構造解析 (2009)
  • Author(s): 平野優、Long-jiang Yu、木村行宏、鈴木宏昭、Zheng-Yu Wang、三木邦夫
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 豊中
• [Presentation] 光合成光捕集反応中心複合体におけるCa^<2+>結合部位の検証 (2009)
  • Author(s): 加藤悟吾、Long-jiang Yu、平野優、木村行宏、Zheng-Yu Wang.
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 豊中
• [Presentation] 好熱性光合成細菌由来のFlavocytochrome cとCytochrome c'の結晶構造解析と機能評価 (2009)
  • Author(s): 源氏梨恵、平野優、木村行宏、木村綾乃、三木邦夫、大友征宇
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
• [Presentation] Calcium ions are involved in thermal stability and unusual Q_y, absorption of core light-harvesting complexes from Thermochromatium tepidum (2008)
  • Author(s): 木村行宏
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-05
• [Presentation] 好熱性紅色光合成細菌Thermochromatium tepidum由来光捕集反応中心複合体におけるカルシウムイオンの構造的、機能的役割 (2008)
  • Author(s): 木村行宏、平野優、Long-jiang Yu、鈴木宏昭、河村悠貴、大野隆、Jian-Ping Zhang、Zheng-Yu Wang.
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
• [Presentation] 好熱性紅色光合成細菌Thermochromatium tepidum由来cytochromeタンパク質flavocytochrome c551, cytochrome c'の結晶構造 (2008)
  • Author(s): 平野優、木村行宏、高崎将充、牛島杏子、木村綾乃、源氏梨恵、鈴木秀明、三木邦夫、大友征宇
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 好熱性紅色光合成細菌Thermochromatium tepidum光捕集反応中心複合体のカルシウムイオンによる熱耐性獲得機構 (2008)
  • Author(s): 木村行宏、平野優、Long-jiang Yu、大野隆、Zheng-Yu Wang.
  • Organizer: 特定領域研究「生体超分子構造」第5回公開シンポジウム
  • Place of Presentation: 筑波
• [Presentation] 好熱性光合成細菌の光捕集反応中心複合体の結晶構造解析とカルシウム結合部位の特定 (2008)
  • Author(s): Long-jiang Yu、平野優、木村行宏、Zheng-Yu Wang.
  • Organizer: 特定領域研究「生体超分子構造」第5回公開シンポジウム
  • Place of Presentation: 筑波
• [Book] Photochemistry : UV/VIS Spectroscopy, Photochemical Reactions and Photosynthesis (2011)
  • Author(s): Y.Yamauchi
  • Publisher: NOVA Science Publisher(in press)
• [Book] Photosystem at high temperature -mechanisms of adaptation and damage
  • Author(s): Yamauchi, Y. Kimura, Y.
  • Publisher: NOVA Science(in press)
• [Remarks] ホームページ等
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