Dissection of mammalian mitochondrial protein synthesis system

• Project/Area Number: 20770135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: TOMITA Nono The University of Tokyo, 大学院・新領域創成科学研究科, 助教 (80323450)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 翻訳 / ミトコンドリア

Research Abstract

To obtain the detailed insight on the molecular mechanism on mitochondrial translation, we studied the mechanism of ribosome recycling process, utilizing the in vitro translation system, reconstituted by ourselves. We discovered the novel translation factor EF-G2mt/RRF2mt which mediates ribosome recycling together with human mitochondrial RRF (RRFmt).We also studied the pathogenic mechanism for the mutation in EF-Tumt and EF-Tsmt.

Research Products

• [Journal Article] 哺乳類ミトコンドリアにおける新規翻訳因子の発見 (2010)
  • Author(s): 竹内野乃、上田卓也
  • Journal Title: 生化学 ((印刷中) (掲載確定))
• [Journal Article] Spremulli Analysis of the Functional Consequences of Lethal Mutations in Mitochondrial Translational Elongation Factors (2010)
  • Author(s): Kenta Akama, Brooke E. Christian, Christie N. Jones, *Nono Takeuchi, Linda L.
  • Journal Title: BBA (in press (掲載確定))
• [Journal Article] A bacterial elongation factor G homolog exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi (2010)
  • Author(s): Suematsu T, Yokobori SI, Morita H, Yoshinari S, Ueda T, Kita K, *Takeuchi N, Watanabe YI.
  • Journal Title: Mol Microbiol 75 Pages: 1445-1454
• [Journal Article] A bacterial elongation factor G homolog exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi (2010)
  • Author(s): Suematsu T, Yokobori SI, Morita H, Yoshinari S, Ueda T, Kita K, Takeuchi N, Watanabe YI.
  • Journal Title: Mol Microbiol. 75 Pages: 1445-1454
  • Peer Reviewed
• [Journal Article] Analysis of the Functional Consequences of Lethal Mutations in Mitochondrial Translational Elongation Factors (2010)
  • Author(s): Kenta Akama, Brooke E.Christian, Christie N.Jones, Nono Takeuchi, Linda L.Spremulli
  • Journal Title: Biochimica et Biophysica Acta
  • Peer Reviewed
• [Journal Article] 哺乳類ミトコンドリアにおける新規翻訳因子の発見 (2010)
  • Author(s): 竹内野乃、上田卓也
  • Journal Title: 生化学 (印刷中, 掲載確定)
• [Journal Article] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis. (2009)
  • Author(s): Tsuboi M., Morita H., Nozaki Y., Akama K., Ueda T., Ito K., Nierhaus KH., *Takeuchi N.
  • Journal Title: Molecular Cell 28 Pages: 502-10
• [Journal Article] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis (2009)
  • Author(s): Tsuboi M., Morita H., Nozaki Y., Akama K., Ueda T., Ito K., Nierhaus KH., Takeuchi N.
  • Journal Title: Molecular Cell 28 Pages: 502-10
  • Peer Reviewed
• [Journal Article] Low conservation and species-specific evolution of alternative splicing in humans and mice : comparative genomics analysis using well-annotated full-length cDNAs. (2008)
  • Author(s): TaKeda JI, Suzuki Y, SaKate R, Sato Y, Seki M, Irie T, Takeuchi N, Ueda T Nakao M, Sugano S, Gojobori T Innishi T.
  • Journal Title: Nucleic Acids Res. 36 Pages: 6386-95
  • Peer Reviewed
• [Journal Article] The human mitochondrial translation release tactor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC. (2008)
  • Author(s): Ishizawa T, Nozaki Y, Ueda T, *Takeuchi N.
  • Journal Title: Biochem Biophys Res Commun. 373 Pages: 99-103
  • Peer Reviewed
• [Journal Article] Comprehensive detection of human terminal oligo-pyrimidine (TOP) genes and analysis of their characteristics. (2008)
  • Author(s): The human mitochondrial translation release tactor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC.
  • Journal Title: Nucleic Acids Res. 36 Pages: 3707-15
  • Peer Reviewed
• [Journal Article] HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG. (2008)
  • Author(s): Nozaki. Y, Matsunaga N, Ishuzawa T, Ueda T, *Takeuchi N.
  • Journal Title: Genes Cells. 7 Pages: 195-203
  • Peer Reviewed
• [Presentation] Dissection of ribosome recycling mechanism in mammalian mitochondrial protein synthesis (2010)
  • Author(s): Nono Takeuchi
  • Organizer: Current Issues of Ribosomal Structure and Function
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2010-04-09
• [Presentation] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis (2010)
  • Author(s): Hiroyuki Morita, Masafumi Tsuboi, Yusuke Nozaki, Kenta Akama, Takuya Ueda, Koichi Ito, Knud H. Nierhaus, Nono Takeuchi
  • Organizer: 23rd tRNA workshop
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-02-01
• [Presentation] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis (2010)
  • Author(s): Hiroyuki Morita, Masafumi Tsuboi, Yusuke Nozaki, Kenta Akama, Takuya Ueda, Koichi Ito, Knud H.Nierhaus, Nono Takeuchi
  • Organizer: 23^<rd> tRNA workshop
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-02-01
• [Presentation] 哺乳類ミトコンドリアにおけるリボソーム再生因子の作用機序 (2009)
  • Author(s): 森田寛幸、野崎雄介、壺井将史、上田卓也、○竹内野乃
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 東京
  • Year and Date: 2009-12-17
• [Presentation] Functional Dissection of Ribosome Recycling Factor (RRF) in mammalian mitochondria: Role of the N-terminal domain of mitochondrial RRF in ribosome recycling. (2009)
  • Author(s): Hiroyuki Morita, Yusuke Nozaki, Masafumi Tsuboi, Takuya Ueda, Nono Takeuchi
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Functional Dissection of Ribosome Recycling Factor(RRF)in mammalian mitochondria : Role of the N-terminal domain of mitochondrial RRF in ribosome recycling (2009)
  • Author(s): 森田寛幸、野崎雄介、壺井将史、上田卓也、竹内野乃
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] EF-G2MT IS AN EXCLUSIVE RECYCLING FACTOR IN MAMMALIAN MITOCHONDRIAL PROTEIN SYNTHESIS EMBO (2009)
  • Author(s): Masafumi Tsuboi, Hiroyuki Morita, Yusuke Nozaki, Kenta Akama, Takuya Ueda, Koichi Ito, Knud H. Nierhaus, Nono Takeuchi
  • Organizer: Conference Series on Protein Synthesis and Translational Control
  • Place of Presentation: Heiderberg, Germany
  • Year and Date: 2009-09-12
• [Presentation] EF-G2mt is an Exclusive Recycling Factor in Mammalian Mitochondrial Protein Synthesis (2009)
  • Author(s): Masafumi Tsuboi, Hiroyuki Morita, Yusuke Nozaki, Kenta Akama, Takuya Ueda, Koichi Ito, Knud H.Nierhaus, Nono Takeuchi
  • Organizer: EMBO Conference Series on Protein Synthesis and Translational Control
  • Place of Presentation: Heiderberg, Germany
  • Year and Date: 2009-09-12
• [Presentation] 哺乳類ミトコンドリアにおけるリボソーム再生因子の作用機序 (2009)
  • Author(s): 森田寛幸、野崎雄介、壺井将史、上田卓也、○竹内野乃
  • Organizer: 日本RNA学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-29
• [Presentation] RIBOSOME RECYCLING PROCESS IN MAMMALIAN MITOCHONDRIA : FUNCTIONAL SPLIT OF EF-G (2009)
  • Author(s): Masafumi Tsuboi, Hiroyuki Morita, Yusuke Nozaki, Takuya Ueda, Koichi Ito, Knud H. Nierhaus, and Nono Takeuchi
  • Organizer: International workshop " Experimental Strategies for Ribosome Research "
  • Place of Presentation: Ringberg (Germany)
  • Year and Date: 2009-04-20
• [Presentation] 哺乳類ミトコンドリア蛋白質合成系におけるリボソーム再生機構 (2008)
  • Author(s): 壺井将史、野崎雄介、森田寛幸、上田卓也、伊藤耕一、竹内野乃
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-19
• [Presentation] 哺乳類ミトコンドリア生体外蛋白質合成系の構築および基礎的応用 (2008)
  • Author(s): 赤間健太、松永興手、甲村明博、岡本有加、上田卓也、竹内野乃
  • Organizer: 日本ミトコンドリア学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-19
• [Presentation] 哺乳類ミトコンドリア蛋白質合成系におけるリボソーム再生機構 (2008)
  • Author(s): 壺井将史、野崎雄介、森田寛幸、上田卓也、伊藤耕一、竹内野乃
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 哺乳類ミトコンドリア生体外蛋白質合成系の構築および基礎的応用 (2008)
  • Author(s): 赤間健太、松永典子、中村明博、岡本有加、上田卓也、竹内野乃
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] RIBOSOME RECYCLING PROCESS IN MAMMALIAN MITOCHONDRIA : FUNCTIONAL SPLIT OF EF-G (2008)
  • Author(s): Masafurni Tsuboi, Hiroyuki Morita, Yusuke Nozaki, Takuya Ueda, Koichi Ito, and Nono Takeuchi
  • Organizer: Translational control meeting
  • Place of Presentation: Cold Spring Harbor (U.S.A.)
  • Year and Date: 2008-09-04
• [Presentation] ESTABLISHMENT OF IN VITRO MAMMALIAN MITOCHONDRIAL TRANSLATION SYSTEM (2008)
  • Author(s): Noriko Matsunaga, Akihiro Nakamura, Yusuke Nozaki, Masafumi Tsuboi, Kenta Akama, Tetsuya Kotani, Yuka Okamoto, Takuya Ueda, and Nono Takeuchi
  • Organizer: Translational control meeting
  • Place of Presentation: Cold Spring Harbor (U.S.A.)
  • Year and Date: 2008-09-04
• [Presentation] 哺乳類ミトコンドリ・ア蛋白質合成系におけるリボソーム再生機構 (2008)
  • Author(s): 壺井将史、野崎雄介、森田寛幸、上田卓也、伊藤耕一、竹内野乃
  • Organizer: 日本RNA学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-25
• [Remarks]
  • URL: http://www.k.u-tokyo.ac.jp/news/20090824press.html#top
• [Remarks]
  • URL: http://www.k.u-tokyo.ac.jp/news/20090824press.html#top