The role of MLDP in lipid metabolism on lipid droplets.

• Project/Area Number: 20770160
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: University of Hyogo
• Principal Investigator: YAMAGUCHI Tomohiro University of Hyogo, 薬学部, 准教授 (50347530)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞構造・機能 / 脂質 / 中性脂肪 / 脂肪滴 / 細胞・組織 / 蛋白質 / 脂質代謝 / MLDP

Research Abstract

Lipid droplet (LD), an intracellular storage depot of lipids, is also an active organelle involved in diverse cellular processes, such as membrane traffic and lipid metabolism. Their properties are highly variable among tissues and are possibly determined by the PERILIPIN family of LD-binding proteins. We identified a new member of this family, named MLDP (also called Perilipin 5) and found that it is highly enriched in the heart. MLDP knockout mice completely lacked LDs in the heart, substantiating the essential role of MLDP in keeping heart LDs.

Research Products

• [Journal Article] Crucial role of CGI-58/α/β hydrolase domain-containing protein 5 in lipid metabolism. (2010)
  • Author(s): Yamaguchi T.
  • Journal Title: Biol.Pharm.Bull. 33 Pages: 342-325
  • Peer Reviewed
• [Journal Article] Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in the liver. (2010)
  • Author(s): Takahashi K., Sasabe N., Ohshima K., Kitazato K., Kato R., Masuda Y., Tsurumaki M., Obama T., Okudaira S., Aoki J., Arai H., Yamaguchi T., Itabe H.
  • Journal Title: J.Lipid Res. 51 Pages: 2571-2580
  • Peer Reviewed
• [Journal Article] Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in (2010)
  • Author(s): Takahashi K., et al
  • Journal Title: Journal of Lipid Research Volume: 51 Pages: 2571-80
  • Peer Reviewed
• [Journal Article] Crucial role of CGI-58/alpha/beta hydrolase domain-containing protein 5 in lipid metabolism (2010)
  • Author(s): Yamaguchi T
  • Journal Title: Biol Pharm Bull. 33 Pages: 342-345
  • NAID: 130000248078
  • Peer Reviewed
• [Journal Article] Activation of hormone-sensitive lipase requires two steps, protein phosphorylation and binding to the PAT-1 domain of lipid droplet coat proteins. (2009)
  • Author(s): Wang H., Hu L., Dalen K., Dorward H., Marcinkiewicz A., Russell D., Gong D., Londos C., Yamaguchi T., Holm C., Rizzo MA., Brasaemle D., Sztalryd C.
  • Journal Title: J.Biol.Chem. 284 Pages: 32116-32125
  • Peer Reviewed
• [Journal Article] Chanarin-Dorfman syndrome : Deficiency in CGI-58, a lipid droplet-bound coactivator of lipase. (2009)
  • Author(s): Yamaguch T., Osumi T.
  • Journal Title: Biochim Biophys Acta. 1791 Pages: 519-523
  • Peer Reviewed
• [Journal Article] Chanarin-Dorfman syndrome : deficiency in CGI-58, a lipid droplet-bound coactivator of lipase. (2009)
  • Author(s): Yamaguchi T., Osumi T.
  • Journal Title: Biochim Biophys Acta. 1791 Pages: 519-523
  • Peer Reviewed
• [Journal Article] FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets. (2008)
  • Author(s): Nishino N., Tamori Y., Tateya S., Kawaguchi T., Shibakusa T., Mizunoya W., Inoue K., Kitazawa R., Kitazawa S., Matsuki Y., Hiramatsu R., Masubuchi S., Omachi A., Kimura K, Saito M., Amo T., Ohta S., Yamaguchi T., Osumi T., Cheng J., Fujimoto T., Nakao H., Nakao K., Aiba A., Okamura H., Fushiki T, Kasuga M.
  • Journal Title: J.Clin.Invest. 118 Pages: 2808-2821
  • Peer Reviewed
• [Journal Article] Perilipin, a critical regulator of fat storage and breakdown, is a target gene of estrogen receptor-related receptorα. (2008)
  • Author(s): Akter MH., Yamaguchi T., Hirose F., Osumi T.
  • Journal Title: Biochem.Biophys.Res.Commun. 368 Pages: 563-568
  • Peer Reviewed
• [Journal Article] Perilipin, a critical regulator of fat storage and breakdown, is a target eene of estrogen recentor-related receptor aloha (2008)
  • Author(s): Akter MH
  • Journal Title: Biochem. Biophys. Res. Commun 368 Pages: 563-568
  • Peer Reviewed
• [Journal Article] FSP27 contributes to efficient energy storage in murine white adipocyt es by promoting the formation of unilocular lipid droplets (2008)
  • Author(s): Nishino N
  • Journal Title: J. Clin. Invest 118 Pages: 2808-2821
  • Peer Reviewed
• [Presentation] 脂肪滴局在タンパク質PERILIPINファミリーによる脂質代謝制御 (2011)
  • Author(s): 山口智広
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] 脂肪分解刺激に応答した脂肪滴局在タンパク質の細胞内動態の解析 (2009)
  • Author(s): 山口智広
  • Organizer: 日本脂質生化学会
  • Place of Presentation: 名古屋
• [Presentation] 脂肪分解刺激に対する脂肪滴表面タンパク質の応答の解析 (2008)
  • Author(s): 橋本健志
  • Organizer: 第81回日本生化学会大会BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Book] 分子糖尿病学の進歩-基礎から応用まで-2008 (2008)
  • Author(s): 山口智広、大隅隆, 他
  • Publisher: 金原出版
• [Remarks] ホームページ等
  • URL: http://www.showa-u.ac.jp/sch/pharm/major/biolchem/index.html