Research on factors of the ubiquitin/proteasome system required for maintaining the nuclear homeostasis in the quiescence

• Project/Area Number: 20770167
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Okinawa Institute of Science and Technology
• Principal Investigator: TAKEDA Kojiro Okinawa Institute of Science and Technology, 研究員 (90426578)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: G0期 / タンパク質分解 / プロテアソーム / オートファジー / ミトコンドリア / 酸化ストレス / 寿命 / ユビキチン / GO期 / 細胞周期 / 核 / 静止期

Research Abstract

分裂酵母をモデル生物として増殖静止期の細胞生存維持におけるタンパク質分解の重要性を研究した。ユビキチン/プロテアソーム系とオートファジーという細胞内の主要なタンパク質分解システムは、有害な酸化ストレスの蓄積から、静止期特異的に細胞を協調して守っている事がわかった。酸化ストレスの蓄積は核やミトコンドリアに顕著であり、これらの細胞内小器官の質的な維持にタンパク質分解系が深く関わっていることが予想される。

Research Products

• [Journal Article] 分裂酵母静止期細胞におけるプロテアソームとオートファジーに協調:ミトコンドリア品質管理と寿命維持 (2010)
  • Author(s): 武田鋼二郎
  • Journal Title: 細胞工学 29 Pages: 429-430
• [Journal Article] In quiescence of fission yeast, autophagy and the proteasome collaborate for mitochondrial maintenance and longevity (2010)
  • Author(s): Takeda, K, Yanagida, M
  • Journal Title: Autophagy
• [Journal Article] Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast (2010)
  • Author(s): Takeda, K., Yoshida, T., Kikuchi, S., Nagao, K., Kokubu, A., Oluskal, T., Villar-Briones, A., Yanagida, M
  • Journal Title: Proc Natl Acad Sci USA 107(8) Pages: 3540-3545
  • NAID: 120002949737
  • Peer Reviewed
• [Journal Article] Synergistic roles of the proteasome and autophagy for mitochondrialmaintenance and chronological life span in fission yeast (2010)
  • Author(s): Takeda, T., Yoshida, M., Kikuchi, S., Nagao, K., Kokubu, A., Pluskal, T., Villar-Briones, A., Nakamura, T., Yanagida, M.
  • Journal Title: Proceedings of the National Academy of Sciences 108 Pages: 3540-3545
  • Peer Reviewed
• [Journal Article] In quiescence of fission yeast, autophagy and the proteasome collaboratefor mitochondrial maintenance and longevity (2010)
  • Author(s): Takeda, K., Yanagida, M.
  • Journal Title: Autophagy誌掲載確定
• [Journal Article] 分裂酵母静止期細胞におけるプロテアソームとオートファジーの協調:ミトコンドリア品質管理と寿命維持 (2010)
  • Author(s): 武田鋼二郎
  • Journal Title: 細胞工学 29 Pages: 429-430
• [Journal Article] Genetic control of cellular quiescence in S. pombe (2009)
  • Author(s): Sajiki, K., Hatanaka, M., Nakamura, T., Takeda, K., Shimanuki, M., Yoshida, T., Hanyu, Y., Nakaseko, Y., Yanagida, M
  • Journal Title: J Cell Sci 122(9) Pages: 1418-1429
  • Peer Reviewed
• [Journal Article] Genetic control of cellular quiescence in S. pombe (2009)
  • Author(s): Sajiki, K., et.al.
  • Journal Title: Journal of Cell Science 122 Pages: 1418-1429
  • Peer Reviewed
• [Presentation] タンパク質分解系の協調によるミトコンドリア品質管理と寿命維持～分裂酵母をモデルとしたアプローチ～ (2010)
  • Author(s): 武田鋼二郎
  • Organizer: 京都大学医学部老年内科学セミナー
  • Place of Presentation: 京都大学
  • Year and Date: 2010-04-23
• [Presentation] タンパク質分解系の協調によるミトコンドリア品質管理と寿命維持～分裂酵母をモデルとしたアプローチ～ (2010)
  • Author(s): 武田鋼二郎
  • Organizer: 京都大学医学部老年内科学セミナー(招待講演)
  • Place of Presentation: 京都大学医学部
  • Year and Date: 2010-04-23
• [Presentation] Mitochondrial regulations and the maintenance of S. pombe cells under limited nutrient condition (2010)
  • Author(s): Kojiro Takeda
  • Organizer: The 3rd Workshop on transcriptional and signaling network of lifestyle related diseases
  • Place of Presentation: Kamakura
  • Year and Date: 2010-02-20
• [Presentation] The proteasome and autophagy system cooperatively contributes to the maintenance of G0 phase by reducing cellular oxidative stress (2009)
  • Author(s): Kojiro Takeda
  • Organizer: 4th Internaltional Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: Okinawa, Japan
• [Presentation] The proteasome and autophagy system cooperatively contributes to the maintenance of G0 phase by reducing cellular oxidative stress (2009)
  • Author(s): Kojiro Takeda
  • Organizer: The 5th International Fission Yeast Meeting
  • Place of Presentation: 東京
• [Presentation] The proteasome and autophagy system cooperatively contributes to the maintenance of GO phase by reducing cellular oxidative stress (2009)
  • Author(s): Kojiro Takeda
  • Organizer: The 5th International Fission Yeast Meeting Tokyo, Japan
  • Place of Presentation: Tokyo, Japan
• [Presentation] The quality control of mitochondria is achieved by Ub/proteasome and autophagy in fission yeast G0 phase (2008)
  • Author(s): Kojiro Takeda
  • Organizer: 8th J-mit meeting
  • Place of Presentation: 東京女子医科大学
  • Year and Date: 2008-12-18
• [Presentation] The quality control of mitochondria is achieved by Ub/Proteasome and autophagy in fission yeast G0 phase (2008)
  • Author(s): Takeda, K.
  • Organizer: 8^<th> J-mit
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-18
• [Presentation] The quality control of mitochondria is achieved by Ub/proteasome and autophagy in fission yeast G0 phase (2008)
  • Author(s): 武田鋼二郎
  • Organizer: The 31st annual meeting of the Molecular Biology Society of Japan
  • Place of Presentation: 神戸
• [Presentation] The selective degradation of mitochondria is induced by ubiquitin/proteasome dysfunction in fission yeast G0 phase (2008)
  • Author(s): Kojiro Takeda
  • Organizer: EUROMIT7
  • Place of Presentation: ストックホルム
• [Presentation] The selective degradation of mitochondria is induced by ubiauitin/proteasome dysfunction in fission yeast G0 phase (2008)
  • Author(s): Takeda, K.
  • Organizer: EUROMIT 7
  • Place of Presentation: Stockholm, Sweden
• [Presentation] The quality control of mitochondria is achieved by Ub/Proteasome and autophagy in fission yeast G0 phase (2008)
  • Author(s): Takeda, K.
  • Organizer: The 31st annual meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Kobe, Japan
• [Remarks] 研究成果が2010年2月に、沖縄タイムズ、京都新聞、読売新聞で報道された