The imaging analysis of the mechanism of cell differentiation during mouse blastocyst formation

• Project/Area Number: 20770176
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: KOMATSU Kouji National Institute for Basic Biology, 初期発生研究部門, 特別協力研究員 (40456893)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞分化 / 胚盤胞 / 内部細胞塊 / 栄養外胚葉 / Nanog / 着床前胚 / Oct4 / Cdx2

Research Abstract

We analyzed the localization of Oct3/4 (Oct4), Nanog and Cdx2, which are expressed in ICM and TE respectively in the late blastocyst. relating to the precise position of cells within the embryo to study early steps of differentiation of cells. From results of this analysis, it was suggested that the initiation and the maintenance of Cdx2 expression is relating to the relative positions of cells in the embryo after 8-cell stage. On the other hand, Nanog expression was randomly upregulated regardless of cell position from 8-cell to early 16-cell stage, and the expression was preferentially upregulated in inner cells from late 16-cell to 32-cell stage.

Research Products

• [Journal Article] AndreGoffinet Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fatal hydrocephalus (2010)
  • Author(s): Fadel Tissir, Yibo Qu, Mireille Montcouquiol, Libing Zhou, Kouji Komatsu, Dongbo Shi, Toshihiko Fujimori, Jason Labeau, Donatienne Tyteca, PierreCourtoy, Yves Poumay, Tadashi Uemura
  • Journal Title: Nature Neuroscience 13 Pages: 700-707
• [Journal Article] Morphological organization of the mouse preimplantation embryo (2009)
  • Author(s): Toshihiko Fujimori, Yoko Kurotaki, Kouji Komatsu, Yo-ichi Nabeshima
  • Journal Title: Reproductive Science 16(2) Pages: 171-177
• [Journal Article] Atsuko Sehara-Fujiwsawa Roles of meltrin-beta / ADAM19 in progression of Schwann cell differentiation and myelination during sciatic nerve regeneration (2009)
  • Author(s): Shuji Wakatsuki, Norihiro Yumoto, Koji Komatsu, Toshiyuki Araki
  • Journal Title: Journal of Biological Chemistry 284(5) Pages: 1957-1966
• [Journal Article] Morphological organizaton of the mouse preimplantation embryo (2009)
  • Author(s): Toshihiko Fujimori, et.al.
  • Journal Title: Reproductive Science 16(2) Pages: 171-177
  • Peer Reviewed
• [Journal Article] Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, Leading of fatal hydrocephalus
  • Author(s): Fadel Tissir et al.
  • Journal Title: Nature Neuroscience (印刷中(査読後、受理済))
  • Peer Reviewed
• [Presentation] The analysis of cell movement and expression of Oct4, Nanog and Cdx2 during mouse blastocyst formation (2008)
  • Author(s): Kouji Komatsu, Yo-ichi Nabeshima, Toshihiko Fujimori
  • Organizer: Frontiers in Developmental Biology
  • Place of Presentation: Giens(France)
  • Year and Date: 2008-09-14
• [Presentation] The analysis of cell movement and expression of Oct4 ; Nanog and Cdx2 during mouse blastocyst formation (2008)
  • Author(s): 小松紘司
  • Organizer: Frontiers in Developmental Biology(日仏合同発生学会)
  • Place of Presentation: Giens (France)
  • Year and Date: 2008-09-14
• [Presentation] The expression of Cdx2 change according to the position of cells in mouse blastocyst (2008)
  • Author(s): 小松紘司、鍋島陽一、藤森俊彦
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島市
  • Year and Date: 2008-05-28
• [Presentation] The expression of Cdx2 changes according to the position of cells in Mouse blastocyst formation (2008)
  • Author(s): 小松紘司
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島県
  • Year and Date: 2008-05-28