Analysis of undifferentiated hematopoietic cells from hematopoietic tissues of mouse embryo and role of the cells in hematopoiesis on ontogeny
| Project/Area Number |
20770181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
NOBUHISA Ikuo Kumamoto University, 難治疾患研究所, 准教授 (40332879)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 細胞分化 / 造血 / AGM / 血液発生 |
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| Research Abstract |
We try the identification of the undifferentiated hematopoietic cells on mouse embryos. In the AGM regions, which show the emergence of cells containing the long-term repopulating activity in irradiated adult mice, CD45lowc-Kithigh cells had the highest ability to form hematopoietic cell colonies in vitro. We found that CD45lowc-Kithigh cells were identified in the stages of showing hematopoiesis in the AGM regions. The CD45lowc-Kithigh cells were also identified in yolk san and fetal liver. In the placenta, the hematopoietic activity of CD45highc-Kithigh cells was highest.
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Report
(3 results)
Research Products
(15 results)
