Evolutionary conserved mechanisms of cell movements in metazoans : insights from the ctenophore Brachyury

• Project/Area Number: 20770190
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Hokkaido University
• Principal Investigator: YAMADA Atsuko Hokkaido University, 低温科学研究所, 研究員 (60333217)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞運動 / 進化 / Brachyury遺伝子 / クシクラゲ / ツメガエル / Delta-Notchシグナル / プリンヌクレオシドホスホリラーゼ / クシククラゲ / Brachyury

Research Abstract

Injection of RNA encoding a dominant-interfering construct of M1Bra, a Brachyury (Bra) ortholog of the ctenophore Mnemiopsis leidyi, into Xenopus embryos revealed that MlBra could regulate Xenopus gastrulation in the same way as the endogenous Xenopus Bra (Xbra), suggesting that the role of Bra in cell movements is conserved over the species. In addition, microarray analyses in Xenopus embryos suggested that Delta-Notch signaling components and the purine-nucleoside phosphorylase gene may function downstream of Bra.

Research Products

• [Journal Article] Highly conserved functions of the Brachyury gene on morphogenetic movements : Insight from the early-diverging phylum Ctenophora (2010)
  • Author(s): Yamada, A, Martindale, M.Q., Fukui, A., Tochinai, S.
  • Journal Title: Developmental Biology Vol.339 Pages: 212-222
  • NAID: 120002004275
  • URL: http://hdl.handle.net/2115/42805.
  • Peer Reviewed
• [Journal Article] Highly conserved functions of the Brachyury gene on morphogenetic movements : Insight from the early-diverging phylum Cteonphora (2010)
  • Author(s): Atsuko Yamada
  • Journal Title: Developmental Biology 339 Pages: 212-222
  • Peer Reviewed
• [Presentation] In silico and in vivo surveys of Brachyury target gens in vertebrates (2010)
  • Author(s): Atsuko Yamada, Kanako O.Koyanagi, Hidemi Watanabe
  • Organizer: The 3rd International Symposium on Global COE Program of Center for Next-Generation Information Technology Based on Knowledge Discovery and Knowledge Federation
  • Place of Presentation: Hokkaido University, Sapporo, Japan.
  • Year and Date: 2010-01-19
• [Presentation] In silico and in vivo surveys of Brachyury target gens in vertebrates (2010)
  • Author(s): Atsuko Yamada
  • Organizer: The 3rd International Symposium on Global COE Program of Center for Next-Generation Information Technology Based on Knowledge Discovery and Knowledge Federation
  • Place of Presentation: 北海道大学(札幌)
• [Presentation] Brachyuryタンパク質の結合サイト配列を用いたBrachyury標的遺伝子の探索 (2009)
  • Author(s): 山田温子, 小柳香奈子, 渡邉日出海
  • Organizer: 第2回若手研究者支援のための産学協同GCOE国内シンポジウム
  • Place of Presentation: 札幌,京王プラザホテル札幌
  • Year and Date: 2009-10-01
• [Presentation] Study on ancestral roles of the Brachyury gene during metazoan evolution (2009)
  • Author(s): Atsuko Yamada, Mark Q.Martindale, Akimasa Fukui, Hidemi Watanabe, Shin Tochinai
  • Organizer: The 2^<nd> International Symposium on Global COE Program of Center for Next-Generation Information Technology Based on Knowledge Discovery and Knowledge Federation
  • Place of Presentation: Hokkaido University, Sapporo, Japan.
  • Year and Date: 2009-01-20
• [Presentation] Study on ancestral roles of the Brachyury gene during metazoan evolution (2009)
  • Author(s): Atsuko Yamada
  • Organizer: The 2nd International Symposium on Global COE Program of Center for Next-Generation Information Technology Based on Knowledge Discovery and Knowledge Federation
  • Place of Presentation: 北海道大学(札幌)