Positional cloning of flowering time QTLs conferring adaptability to wide cultivation area in Japanese rice cultivars.

• Project/Area Number: 20780008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: HORI Kiyosumi National Institute of Agrobiological Sciences, QTLゲノム育種研究センター, 任期付研究員 (50442827)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: イネ / 出穂期 / QTL / コシヒカリ / ポジショナルクローニング

Research Abstract

Flowering time of rice cultivars has diversified to adjust to the growing season in each local cultivation area. Quantitative trait locus (QTL) analysis revealed that two QTLs (Hd16 and Hd17) were involved in the difference of flowering time between the rice japonica cultivars Koshihikari and Nipponbare. High-resolution mapping and sequence analysis of the genomic regions enabled us to focus on candidate genes for each QTL. Compared to control lines, flowering time in transgenic plants with a genomic fragment containing the Hd16 candidate gene was changed under short and long day-length conditions. Significant digenic interaction of Hd16 was observed with Hd2, which was previously identified as a flowering time QTL in rice.

Research Products

• [Journal Article] Novel QTLs for photoperiodic flowering revealed by using reciprocal backcross inbred lines from crosses between japonica rice cultivars (2008)
  • Author(s): Matsubara K、Kono I、Hori K, Nonoue Y、Ono N、Shomura A、Mizubayashi T、Yamamoto S、Yamanouchi U、Shirasawa K、Nishio T、Yano M
  • Journal Title: Theoretical and Applied Genetics Vol.117 Pages: 935-945
  • Peer Reviewed
• [Journal Article] Novel QTLs for photoperiodic flowering revealed by using recip rocal backcross inbred lines from crosses between japonica rice cultivars. (2008)
  • Author(s): Matsubara K, Kono I, Hori K, Nonoue Y, Ono N, Shomura A, Mizubayashi T, Yamamoto S, Yamanouchi U, Shirasawa K, Nishio T, Yano M
  • Journal Title: Theoretical and Applied Genetics 117 Pages: 935-945
  • Peer Reviewed
• [Presentation] A novel photoperiod sensitivity QTL, Hd16, conferring adaptability to wide cultivation area in rice (2010)
  • Author(s): Hori K、Matsubara K、Ogiso E、Yamanouchi U、Yano M
  • Organizer: Plant and Animal Genome XVIII Conference
  • Place of Presentation: San Diego、USA
  • Year and Date: 2010-01-09
• [Presentation] A novel photoperiod sensitivity QTL, Hd16, conferring adaptability to wide cultivation area in rice (2010)
  • Author(s): Hori K, Matsubara K, Ogiso E, Yamanouchi U, Yano M
  • Organizer: Plant and Animal Genome XVIII Conference
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-01-09
• [Presentation] Detection of novel flowering time QTLs associated with wide adaptability from Japanese rice cultivar Koshihikari (2008)
  • Author(s): Hori K、Matsubara K、Yamanouchi U、Yano M
  • Organizer: Control of Flowering Time and Applications for Plant Breeding
  • Place of Presentation: Salzau、Germany
  • Year and Date: 2008-09-24
• [Presentation] Detection of novel flowering time QTLs associated with wide adaptability from Japanese rice cultivar Koshihikari. (2008)
  • Author(s): Hori K, Matsubara K, Yamanouchi U, Yano M
  • Organizer: Control of Flowering Time and Applications for Plant Breeding
  • Place of Presentation: Salzau, Germany
  • Year and Date: 2008-09-24