Establishment of transient RNAi methods in tree fruit and it's application on evaluation of genes regulating fruit ripening
Project/Area Number |
20780021
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Horticulture/Landscape architecture
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Research Institution | Okayama University |
Principal Investigator |
NAKANO Ryouhei Okayama University, 大学院・自然科学研究科, 准教授 (70294444)
|
Co-Investigator(Renkei-kenkyūsha) |
KUBO Yasutaka 岡山大学, 大学院・自然科学研究科, 教授 (80167387)
USHIJIMA Koichiro 岡山大学, 大学院・自然科学研究科, 教授 (20379720)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | RNAサイレンシング / 果実 / 成熟 / RNAサインレンシング / hair-pin RNAi / 成熟制御因子 / MADS-Box転写因子 / GRAS / カンキツ / 着色関連遺伝子 / ブドウ |
Research Abstract |
Transient RNA silencing technique was suggested to be possible in developing grape berry. When tomato is used as model plant for screening of fruit ripening-associated genes, AP3, TM6, AGL1, GRAS were assumed to be involved in ripening. Involvements of these genes in ripening of grape berry were investigated using the transient RNA silencing technique, although their involvements were not clear. It is suggested that the transient RNA silencing technique would be useful for analysis of ripening-associated genes in grape in future and that deeper investigation of function of these genes for fruit ripening will provide new insight into the study for fruit physiology.
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Report
(4 results)
Research Products
(7 results)