A role of auxin produced by phytopathogenic fungi during biotrophic phase

• Project/Area Number: 20780034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: TANAKA Eiji Ishikawa Prefectural University, 生物資源環境学部, 准教授 (50433199)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 菌類 / 細胞 / 組織 / 感染生理 / 植物 / 活物寄生 / 侵入菌糸 / 組織科学 / オーキシン / イネいもち病菌 / 応答反応

Research Abstract

The rice blast fungus Magnaporthe oryzae requires living plant cells in its early stages of infection and invasion of host tissue. Immunohistological analyses showed that antigens of the anti-indole-3-acetic acid monoclonal antibody were localized in the fungal infection hyphae present in the rice tissue. Rice transformants containing an auxin-inducible reporter construct demonstrated an auxin response around the apex of the infection hyphae after fungal invasion of living tissue. These results suggest that the rice blast fungus produces a small amount of auxin during its biotrophic phase and the invaded rice tissue responds to the exogenous fungal auxin. In addition, it was shown that the biotrophic fungal hyphae in host tissues were able to be visualized species-specifically by using in situ hybridization technique.

Research Products

• [Journal Article] Auxin production by the rice blast fungus and its localization in host tissue. (2011)
  • Author(s): Eiji Tanaka, Hironori Koga, Mari Mori, Masashi Mori.
  • Journal Title: Journal of Phytopathology. In Press.
  • Peer Reviewed
• [Journal Article] Auxin production by the rice blast fungus and its localization in host tissue (2011)
  • Author(s): Eiji Tanaka, Hironori Koga, Mari Mori, Masashi Mori
  • Journal Title: Journal of Phytopathology Volume: 159(In press)
  • Peer Reviewed
• [Journal Article] Specific in situ visualization of the pathogenic endophytic fungus Aciculosporium take, the cause of witches'broom in bamboo (2009)
  • Author(s): Eiji Tanaka.
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 4829-4834
  • Peer Reviewed
• [Journal Article] Specific in situ visualization of the pathogenic endophytic fungus Aciculosporium take, the cause of witches' broom in bamboo (2009)
  • Author(s): Eiji Tanaka
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 4829-4834
  • Peer Reviewed
• [Presentation] イネいもち病菌感染時におけるエキスパンシン発現の組織学的解析 (2009)
  • Author(s): 田中栄爾・古賀博則
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 兵庫県神戸
  • Year and Date: 2009-10-18
• [Presentation] Species-specific visualization of endophytic fungus in host plant tissue by in situ hybridization. (2009)
  • Author(s): Eiji Tanaka.
  • Organizer: Asian Mycological Congress 2009.
  • Place of Presentation: 台湾台中市
• [Presentation] イネいもち病菌感染時におけるエキスパンシン発現の組織学的解析 (2009)
  • Author(s): 田中栄爾・古賀博則
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 神戸市
• [Presentation] イネいもち病菌侵入菌糸の近接部位におけるイネ葉組織のオーキシン応答. (2008)
  • Author(s): 田中栄爾・森真理・森正之・古賀博則
  • Organizer: 植物病理学会関西部会
  • Place of Presentation: 和歌山県
  • Year and Date: 2008-09-18
• [Presentation] In situ hybridizationによるタケ類てんぐ巣病菌(Aciculosporium take)の宿主組織における局在検出 (2008)
  • Author(s): 田中栄爾
  • Organizer: 日本菌学会大会
  • Place of Presentation: 三重県
  • Year and Date: 2008-05-31
• [Presentation] イネいもち病菌侵入菌糸の近接部位におけるイネ葉組織のオーキシン応答 (2008)
  • Author(s): 田中栄爾・森真理・森正之・古賀博則
  • Organizer: 植物病理学会関西部会
  • Place of Presentation: 和歌山市
• [Presentation] Detection and localization of Aciculosporium take, the causal fungus of witches'broom of bamboo, in host tissue by in situ hybridization. (2008)
  • Author(s): Eiji Tanaka.
  • Organizer: Japan-China Pan Asia mycological forum 2008.
  • Place of Presentation: 中国吉林省長春
• [Presentation] In situ hybridizationによるタケ類てんぐ巣病菌(Aciculosporium take)の宿主組織における局在検出 (2008)
  • Author(s): 田中栄爾
  • Organizer: 日本菌学会
  • Place of Presentation: 三重県津市
• [Presentation] Detection and localization of Aciculosporium take, the causal fungus of witches' broom of bamboo, in host tissue by in situ hybridization. (2008)
  • Author(s): Eiji Tanaka
  • Organizer: Japan-China Pan Asia mycological forum 2008
  • Place of Presentation: 中国吉林省長春