| Project/Area Number |
20780050
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University (2009)
The University of Tokyo (2008) |
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Principal Investigator |
NARITA Shin-Ichiro Kyoto University, ウイルス研究所, 特定助教 (30338751)
|
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| Research Collaborator |
TOKUDA Hajime 盛岡大学, 栄養科学部, 教授
ISHIMARU Kotomi 東京大学, 農学生命科学研究科, 大学院生
TERASAKI Takeshi 東京大学, 農学生命科学研究科, 大学院生
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 微生物学 / 細菌細胞表層 / 緑膿菌 / 薬剤耐性 / リポ蛋白質 / 細菌 / 蛋白質 / 局在化シグナル |
|---|
| Research Abstract |
Lipoproteins are located either in the inner or outer membranes of gram-negative bacteria such as Pseudomonas aeruginosa. Membrane localization of lipoproteins in P. aeruginosa is strictly determined by the residues at positions 3 and 4. To establish the sorting rule for lipoproteins in P. aeruinosa, combinations of amino acids at positions 3 and 4 that function as inner membrane retention signals for lipoproteins were selected. It turned out that charged residues such as Lys and Glu present at position 3 caused inner membrane retention of lipoproteins.
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