A novel network regulating the expression of PCB deRradation Renes.

• Project/Area Number: 20780059
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Tohoku Gakuin University
• Principal Investigator: MIYAUCHI Keisuke Tohoku Gakuin University, 工学部, 准教授 (20324014)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: PCB / 転写制御 / Rhodococcus / カタポライト抑制 / カタボライト抑制

Research Abstract

The expression of polychlorinated biphenyls degradation genes in a PCB degrader, Rhodococcus jostii RHA1, is activated in the existence of biphenyl. This activation is repressed in the existence of intermediate metabolites which appear in the downstream pathway. In this study. we revealed that catechol is the metabolite which causes this repression. By making the metabolism flux from catechol smoothly, a PCB degrader which gives the constant degradation activity can be constructed.

Research Products

• [Journal Article] Glucose-mediated transcriptional repression of PCB/biphenyl catabolic genes in Rhodococcus jostii RHA1. (2010)
  • Author(s): Araki N, Niikura Y, Miyauchi K, Kasai D, Masai E, Fukuda M.
  • Journal Title: J Mol Microbiol Biotechnol. 20 Pages: 53-62
  • Peer Reviewed
• [Journal Article] Dual Two-Component Regulatory Systems Are Involved in Aromatic Compound Degradation in a Polychlorinated-Biphenyl Degrader, Rhodococcus jostii RHA1. (2010)
  • Author(s): Takeda, H., Shimodaira, J., Yukawa, K., Hara, N., Kasai, D., Miyauchi, K., Masai, E., Fukuda, M.
  • Journal Title: Journal of Bacteriology. 192 Pages: 4741-4751
  • Peer Reviewed
• [Journal Article] Dual Two-Component Regulatory Systems Are Involved in Aromatic Compound Degradation in a Polychlorinated-Biphenyl Degrader, Rhodococcus jostii RHA1 (2010)
  • Author(s): Takeda, H., Shimodaira, J., Yukawa, K., Hara, N., Kasai, D., Miyauchi, K., Masai, E.; Fukuda, M
  • Journal Title: Journal of Bacteriology Volume: 192 Pages: 4741-4751
  • Peer Reviewed
• [Journal Article] Glucose-mediated transcriptional repression of PCB/biphenyl catabolic genes in Rhodococcus jostii RHA1. (2010)
  • Author(s): Araki N, Niikura Y, Miyauchi K, Kasai D, Masai E, Fukuda M.
  • Journal Title: J Mol Microbiol Biotechnol. Volume: 20 Pages: 53-62
  • Peer Reviewed
• [Presentation] PCB分解菌における分解遺伝子の転写抑制原因物質の同定 (2011)
  • Author(s): 伊藤拓、伊藤匡、遠藤銀朗、福田雅夫、宮内啓介
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-26
• [Presentation] Regulatory mechanism of biphenyl/PCB-degradation gene transcription in Rhodococcus jostii RHA1 (2010)
  • Author(s): J. Shimodaira, K. Miyauchi, H. Takeda, D. Kasai, E. Masai, M. Fukuda
  • Organizer: IBS2010
  • Place of Presentation: Rimini, Italy
  • Year and Date: 2010-09-14 – 2010-09-18
• [Presentation] Rhodococcus jostii RHA1におけるPCB分解遺伝子群転写抑制の原因物質の解明とメカニズムの解析 (2010)
  • Author(s): 伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-06-21
• [Presentation] Rhodococcus jostii RHA1におけるPCB分解遺伝子群転写抑制の原因物質の解明とメカニズムの解析 (2010)
  • Author(s): 伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 東北大学・さくらホール
  • Year and Date: 2010-06-21
• [Presentation] Catabolite Repression of Biphenyl Degradation Genes in PCB Degrader, Rhodococcus jostii RHA1. (2010)
  • Author(s): T.Ito, H.Sagae, K.Nakazana, T.Sugiuchi, S.Ara, G.Endo, K.Miyauchi, M.Fukuda
  • Organizer: ASM General Meeting 2010
  • Place of Presentation: San Diego
  • Year and Date: 2010-05-25
• [Presentation] Catabolite Repression of Biphenyl Degradation Genes in PCB Degrader, Rhodococcus jostii RHA1 (2010)
  • Author(s): T.Ito, H.Sagae, K.Nakazawa, T.Sugiuchi, S.Ara, G.Endo, K.Miyauchi, M.Fukuda
  • Organizer: ASM General Meeting 2010
  • Place of Presentation: Convention Center, San Diego U.S.A
  • Year and Date: 2010-05-25
• [Presentation] Catabolite Repression of Biphenyl Degradation Genes in PCB Degrader, Rhodococcus jostii RHA1 (2010)
  • Author(s): T. Ito, H. Sagae, K. Nakazawa, T. Sugiuchi, S. Ara, G. Endo, K. Miyauchi, M. Fukuda
  • Organizer: ASM General Meeting 2010
  • Place of Presentation: Convention Center, San Diego
  • Year and Date: 2010-05-25
• [Presentation] Rhodococcus jostii RHA1におけるPCB分解遺伝子群転写抑制の原因物質の解明 (2010)
  • Author(s): 伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-28
• [Presentation] Rhodococcus jostii RHA1におけるPCB分解遺伝子群転写抑制の原因物質の解明 (2010)
  • Author(s): 宮内啓介
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京都、東京大学
  • Year and Date: 2010-03-28
• [Presentation] Rhodococcus jostii RHA1におけるPCB分解遺伝子群転写抑制の原因物質の解明 (2010)
  • Author(s): 伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京都, 東京大学
  • Year and Date: 2010-03-28
• [Presentation] Regulatory mechanism of biphenyl/PCB-degradation gene transcription in Rhodococcus jostii RHA1. (2010)
  • Author(s): J.Shimodaira, K.Miyauchi, H.Takeda, D.Kasai, E.Masai, M.Fukuda
  • Organizer: IBS2010
  • Place of Presentation: Rimini, Italy
• [Presentation] PCB分解菌Rhodococcus jostii RHA1における分解遺伝子群転写抑制に関与する物質の同定 (2009)
  • Author(s): 伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 土木学会
  • Place of Presentation: 福岡大学
  • Year and Date: 2009-09-04
• [Presentation] PCB分解菌Rhodococcus jostii RHA1における分解遺伝子群転写抑制に関与する物質の同定 (2009)
  • Author(s): 宮内啓介
  • Organizer: 土木学会
  • Place of Presentation: 福岡県、福岡大学
  • Year and Date: 2009-09-04
• [Presentation] PCB分解菌Rhodococcus jostiiにおける安息香酸存在下でのPCB分解遺伝子群転写制御機構の解析 (2009)
  • Author(s): 寒河江秀和、中澤皓次郎、伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 土木学会東北支部大会
  • Place of Presentation: 東北学院大学
  • Year and Date: 2009-03-07
• [Presentation] PCB分解菌Rhpdpcpccus jostiiにおける安息香酸存在下でのPcB分解遺伝子群転写制御機構の解析 (2009)
  • Author(s): 寒河江秀和
  • Organizer: 土木学会東北支部大会
  • Place of Presentation: 東北学院大学工学部
  • Year and Date: 2009-03-07
• [Presentation] PCB分解菌Rhodococcus jostiiにおける安息香酸存在下でのPCB分解遺伝子群転写制御機構の解析 (2009)
  • Author(s): 寒河江秀和、中澤皓次郎、伊藤拓、宮内啓介、遠藤銀朗、福田雅夫
  • Organizer: 土木学会東北支部大会
  • Place of Presentation: 東北学院大学工学部
  • Year and Date: 2009-03-07
• [Presentation] Regulatory mechanism of biphenyl/PCB-degradation gene transcription in Rhodococcus jostii RHA1 (2009)
  • Author(s): J.Shimodaira, K.Miyauchi, H.Takeda, D.Kasai, E.Masai, M.Fukuda
  • Organizer: IBS2010
  • Place of Presentation: Rimini, Italy