| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Research Abstract |
The dpkA gene from Pseudomonas putida was introduced random mutation by error-prone PCR and the thermostabilized mutant enzymes, V117M and Q302R were obtained. The wildtype enzyme, V117M, Q302R and V117M/ Q302R were purified and characterized. The mutant enzymes showed higher thermal and pH stability than wildtype enzyme, but substrate specificities of mutant enzymes did not change. Therefore, the thermostabilized mutant enzymes were useful for synthesis of various N-methyl-L-amino acids.
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