| Project/Area Number |
20780234
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Boundary agriculture
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| Research Institution | Ube National College of Technology |
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Principal Investigator |
ITO Taiji Ube National College of Technology, 疾患ゲノム研究センター, 講師 (60343109)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 環境ホルモン / 環境技術 / 生体機能利用 / バイオテクノロジー / 発現制御 / 生体分子 / 環境ホルモン受容体タンパク質 / 遺伝子工学 / 高感度検出技術 / 酵母 / 生体機能作用 |
|---|
| Research Abstract |
Endocrine Disruptors are thought to correlate with increase of genital tumors, influence for central nervous system and decrease of the number of sperms. In this study, Nonylphenol existing in our close environment is picked up. The receptor proteins were screened as binding proteins to transcriptional co-activator protein, AIBI, in the presence of Nonylphenol using yeast two-hybrid system. Novel protein NPRI (Nonylphenol receptor 1) was isolated.
Biomolecular interaction assay revealed that NPRI specifically binds to Nonylphenol. NPRI localized mainly in endoplasmic reticulum (ER) under non-stress condition, however, in the presence of Nonylphenol, it partly localized in the nucleus and co-localized with AIBI in LNCap cells.
These results strongly suggested that NPRI, in response to Nonylphenol, would translocate from ER to nucleus to disrupt AIBI function, resulting in endocrine disruption.
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