Roles of glycan recognition in cellular functions

• Project/Area Number: 20780243
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TATENO Hirpaki National Institute of Advanced Industrial Science and Technology, 糖鎖医工学研究センター, 研究員 (30450670)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: レクチン / 糖鎖 / 細胞機能 / 分子認識 / 免疫 / 糖鎖アレイ / 真菌 / 細胞 / 内在化

Research Abstract

全ての生物を構成する細胞は糖鎖で覆われている。細胞表層糖鎖の構造を認識して、細胞-細胞間コミュニケーションを媒介している分子がレクチンであり、糖鎖に結合することにより細胞機能に必須の役割を担っている。本研究では、ヒト免疫系において重要な役割を担っているCタイプレクチン4種について、100種類以上の糖鎖に対する定量的相互作用解析を実施した。その結果、これら4種のレクチンの新規糖鎖リガンドを明らかにすることに成功した。最終的には、これらレクチンが病原真菌や腫瘍細胞の認識、糖タンパク質のクリアランスなどに関与することを明らかにした。

Research Products

• [Journal Article] In situ trans ligands of CD22 identified by glycan-protein photo-crosslinking enabled proteomics. (2010)
  • Author(s): Ramya, T.N., Weerapana, E., Liao, L., Zeng, Y., Tateno, H., Liao, L., Yates, J.R. 3rd, Cravatt, B.F., Paulson, J.C.
  • Journal Title: Mol. Cell Proteomics (in press)
  • Peer Reviewed
• [Journal Article] Dual specificity of Langerin to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain. (2010)
  • Author(s): Tateno, H., Ohnishi, K., Yabe, R., Hayatsu, N., Sato, T., Takeya, M., Narimatsu, H., Hirabayashi, J.
  • Journal Title: J. Biol. Chem. 285 Pages: 6390-6400
  • Peer Reviewed
• [Journal Article] Dual specificity of Langerin to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain (2010)
  • Author(s): Hiroaki Tateno
  • Journal Title: J.Biol Chem. 285 Pages: 6390-6400
  • Peer Reviewed
• [Journal Article] In situ trans ligands of CD22 identified by glycan-protein photo-crosslin king enabled proteomics (2010)
  • Author(s): T.N.C.Ramya
  • Journal Title: Mol.Cell Proteomics 9 Pages: 1339-1351
  • Peer Reviewed
• [Journal Article] The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation. (2009)
  • Author(s): Mikami, K., Yamaguchi, D., Tateno, H., Hu, D., Qin, S.Y., Kawasaki, N., Yamada, M., Matsumoto, N., Hirabayashi, J., Ito, Y., Yamamoto, K.
  • Journal Title: Glycobiology 20 Pages: 310-321
  • Peer Reviewed
• [Journal Article] C-type lectin Mincle is an activating receptor for pathogenic fungus (2009)
  • Author(s): Yamasaki, S., Matsumoto, M., Takeuchi, O., Matsuzawa, T., Ishikawa, E., Sakuma, M., Tateno, H., Uno, J., Hirabayashi, J., Mikami, Y., Takeda, K., Akira, S., Saito, T.
  • Journal Title: Malassezia. Proc. Natl. Acad. Sci. 106 Pages: 1897-1902
  • Peer Reviewed
• [Journal Article] Strict binding specificity of small-sized lectins from the red alga Hypnea japonica for core (alpha1-6) fucosylated N-glycans. (2009)
  • Author(s): Okuyama, S., Nakamura-Tsuruta, S., Tateno, H., Hirabayashi, J., Matsubara, K., Hori, K.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 912-920
  • Peer Reviewed
• [Journal Article] Human C21orf63 is a heparin-binding protein. (2009)
  • Author(s): Mitsunaga, K., Harada-Itadani, J., Shikanai, T., Tateno, H., Ikehara, Y., Hirabayashi, J., Narimatsu, H., Angata, T.
  • Journal Title: J. Biochem. 146 Pages: 369-373
  • Peer Reviewed
• [Journal Article] Comparative analysis of core fucose-binding lectins from Lens culinaris and Pisum sativum using frontal affinity chromatography. (2009)
  • Author(s): Tateno, H., Nakamura-Tsuruta, S., Hirabayashi, J.
  • Journal Title: lycobiology 19 Pages: 527-536
  • Peer Reviewed
• [Journal Article] Structural characterization of a lectin from the mushroom Marasmius oreades in complex with the blood group B trisaccharide and calcium. (2009)
  • Author(s): Grahn, E.M., Winter, H.C., Tateno, H., Goldstein, I.J., Krengel, U.
  • Journal Title: J. Mol. Biol. 390 Pages: 457-466
  • Peer Reviewed
• [Journal Article] N-terminal specific point-immobilization of active proteins by the one-pot NEXT-A method. (2009)
  • Author(s): Ebisu, K., Tateno, H., Kuroiwa, H., Kawakami, K., Ikeuchi, M., Hirabayashi, J., Sisido, M., Taki, M.
  • Journal Title: Chembiochem 10 Pages: 2460-2464
  • Peer Reviewed
• [Journal Article] Comparative analysis of core fucose-binding lectins from Lens culinaris and Pisum sativum using frontal affinity chromatography (2009)
  • Author(s): Hiroaki Tateno
  • Journal Title: Glycobiology 19 Pages: 527-536
  • Peer Reviewed
• [Journal Article] Structural characterization of a lectin from the mushroom Marasmius oreades in complex with the blood group B trisaccharide and calcium (2009)
  • Author(s): Elin M.Grahn
  • Journal Title: J.Mol.Biol. 390 Pages: 457-466
  • Peer Reviewed
• [Journal Article] The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation (2009)
  • Author(s): Kaoru Mikami
  • Journal Title: Glycobiology 20 Pages: 310-321
  • Peer Reviewed
• [Presentation] 細胞グライコミクスの推進と内在性レクチン機能解析 (2009)
  • Author(s): 舘野浩章
  • Organizer: 第7回糖鎖科学コンソーシアム
  • Place of Presentation: 大阪府・豊中市
  • Year and Date: 2009-12-08
• [Presentation] 細胞グライコミクスの推進と内在性レクチン機能解析 (2009)
  • Author(s): Hiroaki Tateno
  • Organizer: 第7回糖鎖科学コンソーシア
  • Place of Presentation: 大阪府・豊中市
  • Year and Date: 2009-12-08
• [Presentation] Quantitative sugar-binding analysis of DC-SIGN-related lectins by frontal affinity chromatography : their extended specificities to agalactosylated N-glycans (2009)
  • Author(s): Rikio Yabe
  • Organizer: 2009 Annual Meeting of the Society for Glycobiology
  • Place of Presentation: USA, San Diego
  • Year and Date: 2009-11-14
• [Presentation] DC-SIGN関連レクチンの定量的相互作用解析:アガラクトN型糖鎖への新規特異性の発見 (2009)
  • Author(s): Rikio Yabe
  • Organizer: 2009 Annual Meeting of the Society for Glycobiology
  • Place of Presentation: USA,San Diego
  • Year and Date: 2009-11-14
• [Presentation] Langerin recognizes malignant and pathogenic cel ls through specificity to sulfated and mannosylated glycans via a single C-typ e carbohydrate recognition domain (2009)
  • Author(s): Tateno, H., Ohnishi, K., Yabe, R., Ha yatsu, N., Sato, T., Takeya, M., Narima tsu, H., Hirabayashi, J.
  • Organizer: 2009 Annual Meeting of the Society for Glyc obiology
  • Place of Presentation: USA, San
  • Year and Date: 2009-11-13
• [Presentation] Langerin recognizes malignant and pathogenic cells through specificity to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain (2009)
  • Author(s): Hiroaki Tateno
  • Organizer: 2009 Annual Meeting of the Society for Glycobiology
  • Place of Presentation: USA,San Diego
  • Year and Date: 2009-11-13
• [Presentation] A consensus rule of galectin-rec ognition: Comprehensive analysis by fro ntal affinity chromatography (2009)
  • Author(s): 岩城隼、舘野浩章、中村-鶴田祥子、内山昇、西望、南澤俊和、小南淳子、中村隆範、平林淳
  • Organizer: 1st Asian Communications of Glycobiology and Glyc otechnology (ACGG) Conference
  • Place of Presentation: つくば
  • Year and Date: 2009-10-30
• [Presentation] A consensus rule of galectin-recognition : Comprehensive analysis by frontal affinity chromatography (2009)
  • Author(s): Jun Iwaki
  • Organizer: 1st Asian Communications of Glycobiology and Glycotechnology (ACGG) Conference
  • Place of Presentation: つくば、AIST
  • Year and Date: 2009-10-30
• [Presentation] Langerinは硫酸化糖鎖とマンノース含有糖鎖への特異性を介して腫瘍細胞と病原真菌に結合する2重特異性を獲得した分子である (2009)
  • Author(s): 舘野浩章
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] DC-SIGN関連レクチンの定量的相互作用解析:アガラクトN型糖鎖への新規特異性の発見 (2009)
  • Author(s): Rikio Yabe
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Langerin recognizes malignant and pathogenic cel ls through specificity to sulfated and mannosylated glycans via a single C-typ e carbohydrate recognition domain (2009)
  • Author(s): Tateno, H., Ohnishi, K., Yabe, R., Ha yatsu, N., Sato, T., Takeya, M., Narima tsu, H., Hirabayashi, J.
  • Organizer: 日墺二国間セミナー
  • Place of Presentation: 横浜、湘南国際村
  • Year and Date: 2009-09-21
• [Presentation] Langerin recognizes malignant and pathogenic cells through specificity to sulfated and mannosylated glycans via asingle C-type carbohydrate recognition domain (2009)
  • Author(s): Hiroaki Tateno
  • Organizer: 日墺二国間セミナ
  • Place of Presentation: 横浜、湘南国際村
  • Year and Date: 2009-09-21
• [Presentation] Frontal affinity chromatography reveals a common binding rule for galec tins (2009)
  • Author(s): 岩城隼、舘野浩章、中村-鶴田祥子、内山昇、西望、南澤俊和、小南淳子、中村隆範、平林淳
  • Organizer: CREST国際シンポジウム「獲得免疫と糖鎖生物学」
  • Place of Presentation: 木更津
  • Year and Date: 2009-03-24
• [Presentation] Quantitati ve analysis of oligosaccharide-binding specificity of DC-SIGN-related lectins reveals their extended specificities to agalactosylated glycans (2009)
  • Author(s): 矢部力朗、舘野浩章、平林淳
  • Organizer: CREST国際シンポジウム「獲得免疫と糖鎖生物学」
  • Place of Presentation: 木更津
  • Year and Date: 2009-03-23
• [Presentation] Quantitative analysis of oligosaccharide-binding specificity of DC-SIGN-r elated lectins reveals their extended specificities to agalactosylated glycans (2009)
  • Author(s): 矢部力朗、舘野浩章、平林淳
  • Organizer: CREST国際シンポジウム「獲得免疫と糖鎖生物学」
  • Place of Presentation: 千葉県木更津市
  • Year and Date: 2009-03-23
• [Presentation] アガラクト糖タンパク質はDC-SIGN関連レクチンの新たな機能性リガンドである (2008)
  • Author(s): 矢部力朗、舘野浩章、平林淳
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12