| Project/Area Number |
20790029
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UEDA Takumi The University of Tokyo, 大学院・薬学系研究科, 助教 (20451859)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 構造生物学 / 生化学 / 膜タンパク質 / 核磁気共鳴法 / 光合成名反応 / タンパク質-タンパク質相互作用 / 電子移動反応 / 転移交差飽和法 / 光合成明反応 / Membrane protein / NMR / photosynthesis / protein-protein interaction / electron transfer / transferred cross saturation |
|---|
| Research Abstract |
Plastocyanin (Pc) efficiently proceeds the photosynthetic light reaction by rapidly transport an electron to photosystem I (PSI). In this study, we successfully prepared cadmium-substituted spinach Pc (Cd-Pc), and determined its PSI-binding site by transferred cross-saturation (TCS) experiments. Our study revealed that oxidation of the cupper ion on Pc resulted in conformational change in loop 1, which causes reduction of the affinity between Pc and PSI, and the reduction of the affinity would enable the rapid dissociation of Pc from PSI, which is important for the efficient electron transport.
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