Characterization of novel regulatory system for the bacteriocin biosynthesis

• Project/Area Number: 20790065
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Hiroshima University
• Principal Investigator: NODA Masafumi Hiroshima University, 大学院・医歯薬学総合研究科, 寄附講座助教 (40457289)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 微生物 / 発現制御 / バイオテクノロジー

Research Abstract

In the present study, a part of the particular bacteriocin biosynthesis regulatory system for brevicin 174A produced by plant-derived lactic acid bacteria was elucidated, and novel bacteriocin immunity protein was determined from the biosynthesis gene cluster.

Research Products

• [Journal Article] Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli (2009)
  • Author(s): Wada, T., Noda, M., Kashiwabara, F., Jeon, H. -J., Shirakawa, A., Yabu, H., Matoba, Y., Kumagai, T, Sugiyama, M
  • Journal Title: Microbiology 155 Pages: 1726-1737
  • Peer Reviewed
• [Journal Article] Characterization of four plasmids harbored in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli (2009)
  • Author(s): Wada, T., et.al.
  • Journal Title: Microbiology (In press)
  • Peer Reviewed
• [Presentation] 植物乳酸菌Lactobacillus brevis 174Aの産生する新奇バクテリオシン (2010)
  • Author(s): 野田正文, 松尾隆広, 的場康幸, 熊谷孝則, 杉山政則
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] 植物乳酸菌Lactobacillus brevis 174Aの産生する新奇バクテリオシン (2010)
  • Author(s): 野田正文
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県岡山市
• [Presentation] クラスIIbバクテリオシンbrevicin 174Aの産生制御の分子機構解明 (2009)
  • Author(s): 古林泰子, 野田正文, 柏原ふみ, 松尾隆広, 全亨濬, 的場康幸, 熊谷孝則, 杉山政則
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 名古屋
• [Presentation] バクテリオシン生産性植物乳酸菌925A株の保有する4種類のプラスミドの構造と機能 (2009)
  • Author(s): 野田正文, 和田貴臣, 全亨濬, 柏原ふみ, 的場康幸, 熊谷孝則, 杉山政則
  • Organizer: 日本乳酸菌学会2009年度大会
  • Place of Presentation: 山梨
• [Presentation] バクテリオシン生産性植物乳酸菌925A株の保有する4種類のプラスミドの構造と機能 (2009)
  • Author(s): 野田正文
  • Organizer: 日本乳酸菌学会2009年度大会
  • Place of Presentation: 山梨県甲府市
• [Presentation] クラスIIbバクテリオシンbrevicin 174Aの産生制御の分子機構解明 (2009)
  • Author(s): 古林泰子
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 愛知県名古屋市