Study on neural differentiation of human mesenchymal stem cells based on glycans

• Project/Area Number: 20790110
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: National Institute of Health Sciences
• Principal Investigator: HASHII Noritaka National Institute of Health Sciences, 生物薬品部, 主任研究官 (20425672)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 医薬品化学 / 再生医学 / 再生医療 / 間葉系幹細胞 / 神経分化 / 糖鎖 / 質量分析

Research Abstract

To find molecular markers during neural differentiation of human mesenchymal stem cell (MSC), first, we developed a quantitative glycan profiling using liquid chromatography/mass spectrometry in combination with the isotope tagging method. And then, we conducted differential glycan analysis between MSC and the neural-lineage cells by the quantitative glycan profiling, and demonstrated that glycans can be used as a molecular marker for discriminating undifferentiated MSC from neural-lineage cells.

Research Products

• [Journal Article] Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry: identification of Lewis x-conjugated glycoproteins in mouse kidney. (2009)
  • Author(s): Hashii, N., Kawasaki, N., Itoh, S., Nakajima, Y., Harazono, A., Kawanishi, T., Yamaguchi, T.
  • Journal Title: J. Proteome Res. 8 Pages: 3415-3429
• [Journal Article] Alteration of N-glycosylation in the kidney in a mouse model of systemic lupus erythematosus: relative quantification of N-glycans using an isotope-tagging method. (2009)
  • Author(s): Hashii, N., Kawasaki, N., Itoh, S., Nakajima, Y., Kawanishi, T., Yamaguchi, T.
  • Journal Title: Immunology 126 Pages: 336-345
• [Journal Article] Glycosylation analysis of IgLON family proteins in rat brain by liquid chromatography and multiple-stage mass spectrometry. (2009)
  • Author(s): Itoh, S., Hachisuka, A., Kawasaki, N., Hashii, N., Teshima, R., Hayakawa, T., Kawanishi, T., Yamaguchi, T.
  • Journal Title: Biochemistry 47 Pages: 10132-10154
• [Journal Article] Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry. Identification of Lewis x-glycoproteins in mouse kidney. (2009)
  • Author(s): Noritaka Hashii, Nana Kawasaki, Satsuki ltoh, Yukari Nakajima, Akira Harazono, Toru Kawanishi, Teruhide Yamaguchi
  • Journal Title: J.Proteome Res. 8 Pages: 3415-3429
  • Peer Reviewed
• [Journal Article] Alteration of N-glycosylation in the kidney of systemic lupus erythematosus model mouse. Relative quantification of N-glycans by using isotope tagging method. (2009)
  • Author(s): Noritaka Hashii, Nana Kawasalci, Satsuki Itoh, Yukari Nakajima, Toru Kawanishi, Teruhide Yamaguchi
  • Journal Title: Immunology 126 Pages: 336-345
  • Peer Reviewed
• [Journal Article] Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry. (2008)
  • Author(s): Harazono, A., Kawasaki, N., Itoh, S., Hashii, N., Matsuishi-Nakajima, Y., Kawanishi, T., Yamaguchi, T.
  • Journal Title: J Chromatogr B Analyt Technol Biomed Life Sci. 869 Pages: 20-30
  • NAID: 10028001892
• [Presentation] 糖タンパク質医薬品の単糖試験法の標準化. (2010)
  • Author(s): 原園景, 小林哲, 川崎ナナ, 伊藤さつき, 多田稔, 橋井則貴, 石井明子, 荒戸照世, 柳原繁弘, 八木有紀, 古賀明子, 津田祐里子, 木村美紀子, 崎田政志, 北村智, 山口秀人, 三村尚志, 村田芳美, 濱詰康樹, 佐藤貴之, 長束俊治, 掛樋一晃, 木下充弘, 渡部沙木絵, 山口照英
  • Organizer: 日本薬学会第130年会
• [Presentation] 糖鎖を指標とした細胞治療薬の特性解析 (2009)
  • Author(s): 橋井則貴
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] 糖鎖プロファイルを指標とした細胞治療薬の特性解析 (2009)
  • Author(s): 橋井則貴
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2009-03-06
• [Presentation] 糖鎖を指標とした細胞治療薬の特性解析. (2009)
  • Author(s): 橋井則貴, 川崎ナナ, 篠原聡, 秦艶, 黄笑宇, 伊藤さつき, 山口照英
  • Organizer: 日本薬学会第129年会
• [Presentation] 糖鎖プロファイルを指標とした細胞治療薬の特性解析. (2009)
  • Author(s): 橋井則貴, 川崎ナナ, 篠原聡, 秦艶, 黄笑宇, 伊藤さつき, 山口照英
  • Organizer: 第8回日本再生医療学会総会
• [Presentation] Role of nuclear sialyl lewis x-conjugated glycoproteins in the proliferation of cancer cell lines (2009)
  • Author(s): Daisuke Takakura, Nana Kawasaki, Yan Qin, Xiaoyu Huang, Satsuki Itoh, Noritaka Hashii, Teruhide Yamaguchi
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] ヒト間葉系幹細胞のThy-1の糖鎖構造解析. (2008)
  • Author(s): 伊藤さつき, 川崎ナナ, 篠原聡, 橋井則貴, 山口照英
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
• [Presentation] d5-フェニルヒドラジンを用いた同位体標識法及び液体クロマトグラフィー/質量分析による糖鎖の定量解析. (2008)
  • Author(s): 橋井則貴, 川崎ナナ, 中島紫, 伊藤さつき, 山口照英
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
• [Book] 蛋白質核酸酵素(糖鎖異常の網羅的解析, 臨時増刊号, 糖鎖情報の独自性と普遍性, 53) (2008)
  • Author(s): 川崎ナナ, 橋井則貴, 山口照英
• [Remarks]
  • URL: http://www.nihs.go.jp/dbcb/dbcb-j.html
• [Remarks]
  • URL: http://www.nihs.go.jp/dbcb/dbcb-j.html