The role of TRP channel in cell death

• Project/Area Number: 20790186
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Kyoto University
• Principal Investigator: NUMATA Tomohiro Kyoto University, 大学院・工学研究科, 助教 (20455223)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分子 / 細胞生理学 / 細胞容積調節 / 細胞死 / TRP / アンモニア血しょう / 酸 / イオンチャネル / ネクローシス / アンモニア血症 / TRPチャネル

Research Abstract

In the present study, I identified that TRPM7 is involved in acid-induced cell death. Furthermore, permeation mechanism of proton in TRPM7 was also revealed. On the other hand, it was revealed that TRPM2 is involved in alkali (ammonia)-induced cell death in heterologously expression system and endogenously expressed TRPM2. Moreover, functional analysis for TRPM2 KO mice suggested that TRPM2 is involved at the ammoniemia.

Research Products

• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. (2010)
  • Author(s): Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T.
  • Journal Title: Proc Natl Acad Sci U S A 107 Pages: 332-337
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. (2010)
  • Author(s): Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A.Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A 107 Pages: 332-337
  • Peer Reviewed
• [Journal Article] Pathophysiology and puzzles of the volume-sensitive outwardly rectifying anion channel. (2009)
  • Author(s): Okada, Y., Sato, K., Numata, T
  • Journal Title: J. Physiol. (London) 587 Pages: 2141-2149
• [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. (2009)
  • Author(s): Kiyonaka, S., Kato, K., Nishida, M., Mio, K., Numaga, T., Sawaguchi, Y., Yoshida, T., Wakamori, M., Mori, E., Numata, T., Ishii, M., Takemoto, H., Ojida, A., Watanabe, K., Uemura, A., Kurose, H., Morii, T., Kobayashi, T., Sato, Y., Sato, C., Hamachi, I., Mori, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A 106 Pages: 5400-5405
• [Journal Article] Structures and variable functions of TRP channels Seikagaku. (2009)
  • Author(s): Numata T, Kozai D, Takahashi N, Kato K, Uriu Y, Yamamoto S, Kaneko T, Shinmoto T, Mori Y.
  • Journal Title: 81 81 Pages: 962-983
• [Journal Article] A pathogenic C-terminus-truncated polycystin-2 mutant enhances receptor-activated Ca2+ entry via association with TRPC3 and TRPC7. (2009)
  • Author(s): Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T, Kanai Y, Kawano M, Wakamori M, Nomura H, Koni I, Yamagishi M, Mori Y
  • Journal Title: J. Biol. Chem. 284 Pages: 34400-34412
  • NAID: 120006334914
• [Journal Article] Pathophysiology and puzzles of the volume-sensitive outwardly rectifying anion channel. (2009)
  • Author(s): Okada Y, Sato K, Numata T
  • Journal Title: J.Physiol.(London) 587 Pages: 2141-2149
  • Peer Reviewed
• [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. (2009)
  • Author(s): Kiyonaka S, Kato K, Nishida M, Mio K, Sumaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A 106 Pages: 5400-5405
  • Peer Reviewed
• [Journal Article] A pathogenic C terminus-truncated polycystin-2 mutant enhances receptor-activated Ca2+ entry via association with TRPC3 and TRPC7. (2009)
  • Author(s): Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T, Kanai Y, Kawano M, Wakamori M, Nomura H, Koni I, Yamagishi M, Mori Y.
  • Journal Title: J Biol Chem. 284 Pages: 34400-34412
  • NAID: 120006334914
  • Peer Reviewed
• [Journal Article] Structures and variable functions of TRP channels (2009)
  • Author(s): Numata T, Kozai D, Takahashi N, Kato K, Uriu Y, Yamamoto S, Kaneko T, Shinmoto T, Mori Y.
  • Journal Title: Seikagaku. 81 Pages: 962-983
  • NAID: 10026299033
• [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound (2009)
  • Author(s): Kiyonaka S, (他8名),Numata T, (他12名)
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 5400-5405
  • Peer Reviewed
• [Journal Article] Proton conductivity through the human TRPM7 channel and its molecular determinants. (2008)
  • Author(s): Numata, T., Okada, Y
  • Journal Title: J. Biol. Chem. 283 Pages: 15097-15103
• [Journal Article] Hypertonicity-induced cation channels rescue cells from staurosporine-elicited apoptosis. (2008)
  • Author(s): Numata, T., Sato, K., Okada, Y., Wehner, F
  • Journal Title: Apoptosis 13 Pages: 895-903
• [Journal Article] Molecular determinants of sensitivity and conductivity of human TRPM7 to Mg2+ and Ca2+. (2008)
  • Author(s): Numata, T., Okada, Y
  • Journal Title: Channels. 2 Pages: 283-286
• [Journal Article] Proton conductivity through the human TRPM7 channel and its molecular determinants (2008)
  • Author(s): T. Numata & Y. Okada
  • Journal Title: J. Biol. Chem 283 Pages: 15097-15103
  • Peer Reviewed
• [Journal Article] Hypertonicity-induced cation channels rescue cells from staurosporine-elicited apoptosis (2008)
  • Author(s): T. Numata, K. Sato, Y. Okada & F. Wehner
  • Journal Title: Apoptosis 13 Pages: 895-903
  • Peer Reviewed
• [Journal Article] Molecular determinants of sensitivity and conductivity of human TRPM7 to Mg2+ and Ca2+ (2008)
  • Author(s): T. Numata & Y. Okada
  • Journal Title: Channels 2 Pages: 283-286
  • Peer Reviewed
• [Presentation] Cation channel channel activity determines cell death in human epithelial cells PAT-CVR Joint Symposium (2009)
  • Author(s): Numata T, Wehner F, Sato K, Okada Y
  • Organizer: 自然科学研究機構
  • Place of Presentation: 岡崎コンファレンスセンター
• [Presentation] Expression of TRPM7 channels switches acidosis-induced cell death from apoptosis to necrosis in human epithelial cells (2009)
  • Author(s): Numata T, Okada Y
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: 国立京都国際会館
• [Presentation] Cation channel channel activity determines cell death in human epithelial cells (2009)
  • Author(s): T Numata, F Wehner, K Sato, Y Okada
  • Organizer: PAT-CVR Joint Symposium
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター
• [Presentation] Expression of TRPM7 channels switches acidosis-induced cell death from apoptosis to necrosis in human (2009)
  • Author(s): T Numata, Y Okada
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: 国立京都国際会館
• [Presentation] Proton conductivity through the human TRPM7 channel FASEB (2008)
  • Author(s): Numata T, Okada Y
  • Organizer: Summer Research Conference
  • Place of Presentation: Snowmass, Colorad, USA
• [Presentation] Proton conductivity through the human TRPM7 channel (2008)
  • Author(s): T. Numata & Y. Okada
  • Organizer: FASEB Summer Research Conference
  • Place of Presentation: Snowmass, Co lorad, USA
• [Remarks]
  • URL: http://www.sbchem.kyoto-u.ac.jp/mori-lab/index.html
• [Remarks]
  • URL: http://www.nios.ac.jp/rvd/
• [Remarks]
  • URL: http://www.sbchem.kyoto-u.ac.jp/mori-lab/index.html