Chemical genetic study of the molecular mechanism for the regulation of osteoblast differentiation

• Project/Area Number: 20790218
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Microbial Chemistry Research Foundation
• Principal Investigator: SAKAMOTO Shuichi Microbial Chemistry Research Foundation, 微生物化学研究所沼津支所, 研究員 (60346070)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 化学遺伝学 / 間葉系幹細胞 / 骨芽細胞 / 天然低分子 / レチノイド / 低分子化合物 / 細胞分化

Research Abstract

Osteoblasts are the cells responsible for bone formation during embryonic development and adult life. Small compounds that could induce osteoblast differentiation might be promising sources of therapies for bone diseases such as osteoporosis. To identify inducers of early osteoblastic marker bone ALP in the pluripotent mesenchymal cells, in-house natural and synthetic chemical libraries were screened. Three small compounds were identified as active molecules and one of these compounds is a novel natural product with retinoid activity named decalpenic acid.

Research Products

• [Journal Article] Decalpenic acid, a novel small molecule from Penicillium verruculosum CR37010, induces early osteoblastic markers in pluripotent mesenchymal cells. (2010)
  • Author(s): Sakamoto,S., Kojima,F., Igarashi,M., Umekita, M., Sawa, R., Kubota, Y., Momose, I., Nakae, K., Yamaguchi, S., Adachi, H., Nishimura, Y., Akamatsu, Y.
  • Journal Title: The Journal of Antibiotics. Vol.63 Pages: 703-708
  • Peer Reviewed
• [Journal Article] Decalpenic acid, a novel small molecule from Penicillium verruculosum CR37010, induces early osteoblastic markers in pluripotent mesenchymal cells. (2010)
  • Author(s): Shuichi Sakamoto, 他11名
  • Journal Title: The Journal of Antibiotics Volume: 63 Pages: 703-708
  • Peer Reviewed
• [Presentation] 新規天然化合物Decalpenic acidはレチノイン酸受容体Rar_gammaを介して間葉系幹細胞における早期骨芽細胞分化マーカーの発現を誘導する (2011)
  • Author(s): 坂本修一、小島蕗子、五十嵐雅之、梅北まや、澤竜一、久保田由美子、百瀬功、中栄功一、安達勇光、西村吉雄、赤松穰
  • Organizer: 日本ケミカルバイオロジー学会第6回年会
  • Place of Presentation: 東京都
  • Year and Date: 2011-05-24
• [Presentation] 間葉系幹細胞の骨芽細胞分化を誘導する微生物代謝産物の探索 (2010)
  • Author(s): 坂本修一、小島蕗子、五十嵐雅之、梅北まや、澤竜一、久保田由美子、百瀬功、中栄功一、山口昭一、安達勇光、西村吉雄、赤松穰
  • Organizer: 日本薬学会第130回年会
  • Place of Presentation: 岡山市
  • Year and Date: 2010-03-29
• [Presentation] 間葉系幹細胞の骨芽細胞分化を誘導する微生物代謝産物の探索 (2010)
  • Author(s): 坂本修一, 他11名
  • Organizer: 日本薬学会第130回年会
  • Place of Presentation: 桃太郎アリーナ(岡山市)
  • Year and Date: 2010-03-29