Generation of safer iPS cells for clinical application

• Project/Area Number: 20790227
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: NAKAGAWA Masato Kyoto University, 再生医科学研究所, 助教 (10379539)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: iPS細胞 / ES細胞 / リプログラミング / 再生医療 / 転写因子

Research Abstract

I tried to generate non-integrated iPS cells using adenovirus expression system. Mouse iPS cells could be obtained by introducing the reprogramming factors by adenoviruses. Adenovirus of Sox2, Klf4, or c-Myc could be used for iPS generation instead of retroviruses. I could not generate iPS cells using Oct3/4 adenoviruses. I will examine the conditions for adenovirus infection and generate iPS cells only by adenovirus expression system.

Research Products

• [Journal Article] Complete genetic correction of iPS cells from duchenne muscular dystrophy. (2010)
  • Author(s): Kazuki, Y., Hiratsuka, M., Takiguchi, M., Osaki, M., Kajitani, N., Hoshiya, H., Hiramatsu, K., Yoshino, T., Kazuki, K., Ishihara, C., Takehara, S., Higaki, K., Nakagawa, M., Takahashi, K., Yamanaka, S., Oshimura M.
  • Journal Title: Molecular Therapy 18 Pages: 386-393
  • Peer Reviewed
• [Journal Article] Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells. (2009)
  • Author(s): Nagata, S., Toyoda, M., Yamaguchi, S., Hirano, K., Makino, H., Nishino, K., Miyagawa, Y., Okita, H., Kiyokawa, N., Nakagawa, M., Yamanaka, S., Akutsu, H., Umezawa, A., Tada, T.
  • Journal Title: Genes Cells 14 Pages: 1395-1404
  • Peer Reviewed
• [Journal Article] 体細胞のゲノム操作によるリプログラミング～人工多能性幹細胞(ips細胞)の現状と今後の展開～ (2009)
  • Author(s): 中川誠人, 山中伸弥
  • Journal Title: ゲノム医学 9 Pages: 167-170
• [Journal Article] Suppression ofinduced pluripotent stem cell generation by the p53-p21 pathway. (2009)
  • Author(s): Hong, H., Takahashi, K., Ichisaka, T., Aoi, T., Kanagawa, O., Nakagawa, M., Okita, K., Yamanaka, S.
  • Journal Title: Nature 460 Pages: 1132-1135
  • Peer Reviewed
• [Journal Article] Variation in the safety of induced pluripotent stem cell lines. (2009)
  • Author(s): Miura, K., Okada, Y., Aoi, T., Okada, A., Takahashi, K., Okita, K., Nakagawa, M., Koyanagi, M., Tanabe, K., Ohnuki, M., Ogawa, D., Ikeda, E., Okano, H., Yamanaka, S.
  • Journal Title: Nature Biotechnology 27 Pages: 743-745
  • Peer Reviewed
• [Journal Article] Orderly hematopoietic development of induced pluripotent stem cells via Flk-1+ hemoangiogenic progenitors. (2009)
  • Author(s): Niwa, A., Umeda, K., Chang, H., Saito, M., Okita, K., Takahashi, K., Nakagawa, M., Yamanaka, S., Nakahata, T., Heike, T.
  • Journal Title: Journal of Cellular Physiology 221 Pages: 367-377
  • Peer Reviewed
• [Journal Article] Roles of Sa114 in the generation of pluripotent stem cells from blastocysts and fibroblasts. (2009)
  • Author(s): Tsubooka, N., Ichisaka, T., Okita, K., Takahashi, K., Nakagawa, M., Yamanaka, S.
  • Journal Title: Genes Cells Pages: 683-694
  • Peer Reviewed
• [Journal Article] 体細胞のゲノム操作によるリプログラミング～人工多能性幹細胞(iPS細胞)の現状と今後の展開～ (2009)
  • Author(s): 中川誠人、山中伸弥
  • Journal Title: ゲノム医学 9 Pages: 167-170
• [Journal Article] Suppression of induced pluripotent stem cell generation by the p53-p21 pathway. (2009)
  • Author(s): Hong, H., Takahashi, K., Ichisaka, T., Aoi, T., Kanagawa, O., Nakagawa, M., Okita, K., Yamanaka, S.
  • Journal Title: Nature 460 Pages: 1132-1135
  • Peer Reviewed
• [Journal Article] Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts. (2009)
  • Author(s): Tsubooka, N., Ichisaka, T., Okita, K., Takahashi, K., Nakagawa, M., Yamanaka, S.
  • Journal Title: Genes Cells 14 Pages: 683-694
  • Peer Reviewed
• [Journal Article] Generation of mouse induced pluripotent stem cells without viral vectors. (2008)
  • Author(s): Okita, K., Nakagawa, M., Hong, H., Ichisaka, T., Yamanaka, S.
  • Journal Title: Science 322 Pages: 949-953
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. (2008)
  • Author(s): Nakagawa, M., Koyanagi, M., Tanabe, K., Takahashi, K., Ichisaka, T., Aoi, T., Okita, K., Mochiduki, Y., Takizawa, N., Yamanaka, S.
  • Journal Title: Nat Biotechnol 26 Pages: 101-106
  • Peer Reviewed
• [Journal Article] Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors (2008)
  • Author(s): Okita, K. Nakagawa, Hyenjong H., Ichisaka, T. & Yamanaka, S.
  • Journal Title: Science 322 Pages: 949-953
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts (2008)
  • Author(s): Nakagawa, M., Koyanagi, Tanabe, K., Takahashi, K., Ichisaka, T. Okita, Mochiduki, Y., Takizawa, N. & Yamanaka, S.
  • Journal Title: Nat Biotechnol 26 Pages: 101-106
  • Peer Reviewed
• [Presentation] Generati on of safer iPS cells by factor X instead of c-MYC (2009)
  • Author(s): 中川誠人、瀧澤奈々子、一阪朋子、杉山逸未、山中伸弥
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Generation of safer iPS cells by factor X instead of c-MYC (2009)
  • Author(s): 中川誠人、瀧澤奈々子、一阪朋子、杉山逸未、山中伸弥
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] ips細胞の再生医療への応用=ips細胞研究の現状と課題= (2009)
  • Author(s): 中川誠人
  • Organizer: 第25回創薬セミナー
  • Place of Presentation: 山梨
  • Year and Date: 2009-07-29
• [Presentation] iPS細胞の再生医療への応用=iPS細胞研究の現状と課題= (2009)
  • Author(s): 中川誠人
  • Organizer: 第25回創薬セミナー
  • Place of Presentation: 山梨
  • Year and Date: 2009-07-29
• [Presentation] 臨床応用へ向けたips細胞研究の現状(iPS cell research for clinical application) (2009)
  • Author(s): 中川誠人
  • Organizer: 化学研究所セミナー
  • Place of Presentation: 京都
  • Year and Date: 2009-07-23
• [Presentation] 臨床応用へ向けたiPS細胞研究の現状(iPS cell research for clinical application) (2009)
  • Author(s): 中川誠人
  • Organizer: 化学研究所セミナー
  • Place of Presentation: 京都
  • Year and Date: 2009-07-23
• [Presentation] ips細胞研究の現状と今後の展開 (2009)
  • Author(s): 中川誠人
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-15
• [Presentation] iPS細胞研究の現状と今後の展開 (2009)
  • Author(s): 中川誠人
  • Organizer: アジレントテクニカルセミナー～高感度化で変わるマイクロアレイの活用法～
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-15
• [Presentation] Generation and characterization of induced Pluripotent stem (iPS) cells (2009)
  • Author(s): 中川誠人
  • Organizer: iCeMS Cross-Disciplinary Seminar
  • Place of Presentation: 京都
  • Year and Date: 2009-07-01
• [Presentation] Generation and characterization of induced pluripotent stem (iPS) cells. (2009)
  • Author(s): 中川誠人
  • Organizer: iCeMS Cross-Disciplinary Seminar
  • Place of Presentation: 京都
  • Year and Date: 2009-07-01
• [Presentation] Mechanism for generation of induced pluripotent stem (iPS) cells (2009)
  • Author(s): 中川誠人
  • Organizer: 第8回日本再生医療総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] Generation of Induced Pluripotent Stem (iPS) Cells (2009)
  • Author(s): Masato Nakagawa
  • Organizer: 3rd Annual JAPANESE-FRENCH FRONTIERS OF SCIENCE SYMPOSIUM
  • Place of Presentation: 神奈川
  • Year and Date: 2009-01-24
• [Presentation] Generation of induced pluripotent stem cells by family genes (2008)
  • Author(s): Masato Nakagawa, Yuji Mochiduki, Kazutoshi Takahashi, Keisuke Okita, Nanako Takizawa, Tomoko Ichisaka, Shinya Yamanaka
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Generation of Induced Pluripotent Stem (iPS) Cells by Sox2, Oct4, Klf4, and c-Myc Transcription Factors and their Family Genes (2008)
  • Author(s): Masato Nakagawa
  • Organizer: The 11th Kyoto University International Symposium 2008(KUIS-11)
  • Place of Presentation: 上海、中国
  • Year and Date: 2008-10-11
• [Presentation] Generation of Induced Pluripotent Stem (iPS) Cells by Sox2, Oct4, Klf4, and c-Myc Transcription Factors and their Family Genes (2008)
  • Author(s): Masato Nakagawa
  • Organizer: The 11th Kyoto University International Symposium 2008 (KUIS-11)
  • Place of Presentation: 中国(上海)
  • Year and Date: 2008-10-11
• [Presentation] iPS細胞の現状と再生医療への応用 (2008)
  • Author(s): 中川誠人
  • Organizer: 第9回日本分子脳神経外科学会
  • Place of Presentation: 京都
  • Year and Date: 2008-08-31
• [Presentation] Generation of induced pluripotent stem (iPS) cells by family genes of Sox, Oct, Klf, and Myc transcription factors (2008)
  • Author(s): Masato Nakagawa, Yuji Mochiduki, Kazutoshi Takahashi, Keisuke Okita, Nanako Takizawa, Tomoko Ichisaka, Shinya Yamanaka
  • Organizer: 6th ISSCR
  • Place of Presentation: フィラデルフィア、米国
  • Year and Date: 2008-06-10
• [Presentation] Generation of induced pluripotent stem (iPS) cells by family genes of Sox, Oct, Klf, and Myc transcription factors (2008)
  • Author(s): Masato Nakagawa, Yuji Mochiduki, Kazutoshi Takahashi, Keisuke Okita, Nanako Takizawa, Tomoko Ichisaka, and Shinya Yamanaka
  • Organizer: 6th ISSCR
  • Place of Presentation: 米国(Philadelphia)
  • Year and Date: 2008-06-10
• [Presentation] ips細胞の展望と課題 (2008)
  • Author(s): 中川誠人
  • Organizer: 日本実験動物科学技術2008
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-15
• [Presentation] iPS細胞の展望と課題 (2008)
  • Author(s): 中川誠人
  • Organizer: 日本実験動物科学技術2008
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-15
• [Presentation] ips細胞の現状と再生医療への応用 (2008)
  • Author(s): 中川誠人
  • Organizer: 第9回日本分子脳神経外科学会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-21
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/rc02/ymho2009/
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立 (2010)
  • Inventor(s): 中川誠人/沖田圭介/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-02-23
  • Overseas
• [Patent(Industrial Property Rights)] 人工多能性幹細胞の樹立効率改善 (2010)
  • Inventor(s): 中川誠人/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-01-22
  • Overseas
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立方法 (2010)
  • Inventor(s): 中川誠人/沖田圭介/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-02-23
  • Overseas
• [Patent(Industrial Property Rights)] 人工多能性幹細胞の樹立効率改善方法 (2010)
  • Inventor(s): 中川誠人/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-01-22
  • Overseas
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立 (2009)
  • Inventor(s): 中川誠人/沖田圭介/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2009-08-07
  • Overseas
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立方法 (2009)
  • Inventor(s): 中川誠人/沖田圭介/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2009-08-07
  • Overseas