Molecular basis of Nucleophosmin mutation in tumor

• Project/Area Number: 20790257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Shimane University
• Principal Investigator: SAKASHITA Gyosuke Shimane University, 医学部, 助教 (00397457)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分子病態学 / ヌクレオフォスミン / リン酸化 / 細胞周期

Research Abstract

Aurora-B phosphorylates NPM1 on S293 and S143 in vitro. However, phosphorylation of S143 was not detected in vivo. Phosphorylation of NPM1 on S293 does not involve in it's subcellular localization, oligomerization and interaction with known NPM1 binding protein such as p19ARF or Nucleolin.

Research Products

• [Journal Article] Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases. (2009)
  • Author(s): Ban R, Matsuzaki H, Akashi T, Sakashita G, Taniguchi H, Park S-Y, Tanaka H, Furukawa K, Urano T.
  • Journal Title: J. Biol. Chem. 284 Pages: 28367-28381
  • Peer Reviewed
• [Journal Article] Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases. (2009)
  • Author(s): Ban R.
  • Journal Title: J.Biol.Chem. 284 Pages: 28367-28381
  • Peer Reviewed
• [Journal Article] Abnormal cytoplasmic dyslocalisation and/or reduction of nucleophosmin protein level rarely occurs in myelodysplastic syndromes. (2008)
  • Author(s): Ishikawa Y, Xu J, Sakashita G, Urano T, Suzuki T, Tomita A, Kiyoi H, Nakamura S, Naoe T.
  • Journal Title: Leuk. Lymphoma. 49 Pages: 2359-2364
  • Peer Reviewed
• [Presentation] 転写制御因子NAC1の機能ドメイン解析と細胞内動態 (2010)
  • Author(s): 加藤太陽
  • Organizer: 第27回染色体ワークショッフ
  • Place of Presentation: 静岡
  • Year and Date: 2010-01-21
• [Presentation] 新規シンタキシンファミリーの同定と機能解析 (2008)
  • Author(s): 坂下暁介、浦野腱
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html