| Project/Area Number |
20790302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKASHIMA Seiji Kyoto University, 医学研究科, 助教 (40396891)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 疾患モデル動物 / 遺伝子改変動物 / 精子幹細胞 / RNA interference |
|---|
| Research Abstract |
To assess whether RNAi mediated gene knock-down could be applied to germline stem cells, RNAi construct for EGFP knock-down was transduced to germline stem cells derived from EGFP transgenic mouse. Although lentivirus mediated RNAi transduction suppressed the EGFP expression to some extent in germline stem cells, the efficiency was not enough to produce the knock-down mouse. Meanwhile, by using RNAi technology developed in this project, it was shown that DNA methyl transferase I is indispensable for the survival of germline stem cells in vitro, suggesting that RNAi mediated knockdown is available for the endogenous genes.
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