Analysis of cell wall constituents of Orientia tsutsugamushi

• Project/Area Number: 20790340
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: NAKAYAMA Keisuke University of Miyazaki, 医学部, 助教 (10347057)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 微生物 / 細菌 / ゲノム / 遺伝子

Research Abstract

Researchers analyzed the chemical composition in whole cells of Orientia tsutsugamushi and concluded that this bacteria have no peptidoglycan. But we revealed that the fosfomycin strongly suppresses the growth of Orientia. This finding helps the development of a new therapeutic strategy of the scrub typhus.

Research Products

• [Journal Article] Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli (2009)
  • Author(s): Y. Ogura, T. Ooka, A. Iguchi, H. Toh, Md. Asadulghani, K. Oshima, T. Kodama, H. Abe, K. Nakayama, K. Kurokawa, T. Tobe, M. Hattori, T. Hayashi
  • Journal Title: Proc. Natl. Acad. Sci. USA 106(42) Pages: 17939-17944
  • Peer Reviewed
• [Journal Article] Development of a multiplex PCR-based rapid typing method 1 for enterohemorrhagic Escherichia coli O157 strains (2009)
  • Author(s): T. Ooka, J. Terajima, M. Kusumoto, A. Iguchi, K. Kurokawa, Y. Ogura, M. Asadulghani, K. Nakayama, K. Murase, M. Ohnishi, S. Iyoda, H. Watanabe, T. Hayashi
  • Journal Title: J. Clin. Microbiol 47(9) Pages: 2888-2894
  • Peer Reviewed
• [Journal Article] Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomes (2009)
  • Author(s): T. Ooka, Y. Ogura, Md. Asadulghani, M. Ohnishi, K. Nakayama, J. Terajima, H. Watanabe, T. Hayashi
  • Journal Title: Genome Res 19 Pages: 1809-1816
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinants (2009)
  • Author(s): Md. Asadulghani, Y. Ogura, T. Ooka, T. Itoh, A. Sawaguchi, A. Iguchi, K. Nakayama, T. Hayashi
  • Journal Title: PLoS Pathog. 5(5)
  • Peer Reviewed
• [Journal Article] Molecular analysis of an extrachromosomal element encoding the C2 toxin gene discovered in Clostridium botulinum type C. (2009)
  • Author(s): Y. Sakaguchi, T. Hayashi, Y. Yamamoto, K. Nakayama, K. Zhang, S. Ma, H. Arimitsu, K. Oguma
  • Journal Title: J. Bacteriol. 191(10) Pages: 3282-3291
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli O157 : prophage-prophage interactions potentiate horizontal transfer of virulence determinants. (2009)
  • Author(s): Md.Asadulghani, et al.
  • Journal Title: PLoS Pathog. 5(5)
  • Peer Reviewed
• [Journal Article] Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomes. (2009)
  • Author(s): T.Ooka, et al.
  • Journal Title: Genome Res. 19 Pages: 1809-1816
  • Peer Reviewed
• [Journal Article] Development of a multiplex PCR-based rapid typing method 1 for enterohemorrhagic Escherichia coli O157 strains. (2009)
  • Author(s): T.Ooka, et al.
  • Journal Title: J.Clin.Microbiol. 47(9) Pages: 2888-2894
  • Peer Reviewed
• [Journal Article] Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. (2009)
  • Author(s): Y.Ogura, et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106(42) Pages: 17939-17944
  • Peer Reviewed
• [Journal Article] The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution (2008)
  • Author(s): K. Nakayama, A. Yamashita, K. Kurokawa, T. Morimoto, M. Ogawa, M. Fukuhara, H. Urakami, M. Ohnishi, I. Uchiyama, Y. Ogura, T. Ooka, K. Oshima, A. Tamura, M. Hattori, T. Hayashi
  • Journal Title: DNA Res. 15 Pages: 185-199
  • Peer Reviewed
• [Journal Article] Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid mapping (2008)
  • Author(s): Y. Ogura, H. Abe, K. Katsura, K. Kurokawa, Asadulghani, A. Iguchi, T. Ooka, K. Nakayama, A. Yamashita, M. Hattori, T. Tobe, T. Hayashi
  • Journal Title: J. Bacteriol 190(21) Pages: 6948-6960
  • Peer Reviewed
• [Journal Article] Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis (2008)
  • Author(s): M. Naito, H. Hirakawa, A. Yamashita, N. Ohara, M. Shoji, H. Yukitake, K. Nakayama, H. Toh, F. Yoshimura, S. Kuhara, M. Hattori, T. Hayashi, K. Nakayama
  • Journal Title: DNA Res. 15 Pages: 215-225
  • NAID: 120006981768
  • Peer Reviewed
• [Journal Article] Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli 055 strains belonging to three distinct lineages (2008)
  • Author(s): Iguchi A., et.al.
  • Journal Title: Microbiology 154(2) Pages: 559-570
  • Peer Reviewed
• [Journal Article] The wholegenome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution (2008)
  • Author(s): Nakayama K., et.al.
  • Journal Title: DNA Res 15 Pages: 185-199
  • Peer Reviewed
• [Journal Article] Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis (2008)
  • Author(s): Naito M., et.al.
  • Journal Title: DNA Res 15 Pages: 215-225
  • Peer Reviewed
• [Journal Article] Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid mapping (2008)
  • Author(s): Ogura Y., et.al.
  • Journal Title: J. Bacteriol 190(21) Pages: 6948-6960
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli 0157 : prophageprophage interactions potentiate horizontal transfer of virulence determinants
  • Author(s): Asadulghani, et.al.
  • Journal Title: PLoS Pathog (in press)
  • Peer Reviewed
• [Presentation] Orientia tsutsugamushiに存在が予想されるペプチドグリカンの解析～Analysis of cell wall structure of Orientia tsutsugamushi～ (2010)
  • Author(s): 中山恵介, 小椋義俊, 大岡唯佑, 林哲也
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] ツツガムシ病病原体のゲノム (2009)
  • Author(s): 中山恵介(特別企画演者)
  • Organizer: 第21回微生物シンポジウム
  • Place of Presentation: 広島県福山市(福山大学社会連携研究推進センター)
  • Year and Date: 2009-09-03
• [Presentation] ツツガムシ病病原体のゲノム (2009)
  • Author(s): 中山恵介(特別企画演者)
  • Organizer: 第21回微生物シンポジウム
  • Place of Presentation: 広島県福山市.
  • Year and Date: 2009-09-03
• [Presentation] オリエンチア属細菌間における比較ゲノム解析 (2009)
  • Author(s): 中山恵介, 小椋義俊, 大岡唯佑, ASADULGHANI, 山崎和子, 村瀬一典, RAKIBUL, 林哲也
  • Organizer: 日本分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] オリエンチア属細菌間における比較ゲノム解析. (2009)
  • Author(s): 中山恵介, 他
  • Organizer: 日本分子生物学会 第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] オリエンチア・ツツガムシに存在が予想されるペプチドグリカンの解析 (2009)
  • Author(s): 中山恵介, 他
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 各古屋
  • Year and Date: 2009-03-13
• [Presentation] オリエンチア・ツツガムシに存在が予想されるペプチドグリカンの解析 (2009)
  • Author(s): 中山恵介, 小椋義俊, 大岡唯祐, アサドルゴニ, 井口純, 村瀬一典, 林哲也
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Orientia tsutsugamushiの比較ゲノム解析 (2009)
  • Author(s): 中山恵介, 黒川顕, 小椋義俊, 大岡唯佑, 林哲也
  • Organizer: 第3回ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] Orientia tsutsugamushi株間における比較ゲノム解析 (2008)
  • Author(s): 中山恵介
  • Organizer: 第2回細菌学・若手コロッセウム
  • Place of Presentation: 神奈川県三浦郡葉山