Analysis of the regulatory proteins of HSV using the mutagenesis in E. coli

• Project/Area Number: 20790361
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TANAKA Michiko National Institute of Infectious Diseases, 感染病理部, 主任研究官 (10356248)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 単純ヘルペスウイルス / 大腸菌 / 組換単純ヘルペスウイルス / 組換え単純ヘルペスウイルス

Research Abstract

Priority of these study is that, new and detail analysis of HSV proteins and reveal the pathogenesis of HSV-1 by using original HSV genome mutagenesis in E. coli. Moreover, combine of MASS analysis, more wide studies, can approach both of virus and host situations. Some candidate proteins are revealed already. More study and repots are expected.

Research Products

• [Journal Article] Differences in the Regulatory and Functional Effects of the Us3 Protein Kinase Activities of Herpes Simplex Virus 1 and 2. (2009)
  • Author(s): Morimoto T., Arii J., Tanaka M., Sata T., Akashi H., Yamada M., Nishiyama Y., Uema M., Kawaguchi Y.
  • Journal Title: Jounal of Virolpgy (83) Pages: 11624-11634
• [Journal Article] Differences in the Regulatory and Functional Effects of the Us3 Protein Kinase Activities of Herpes Simpfex Vieus 1 and 2. (2009)
  • Author(s): Morimoto T., Arii J., Tanaka M., Sata T., Akashi H., Yamada M., Nishiyama Y., Uema M., Kawaguchi Y..
  • Journal Title: Jounal of Virolpgy 83 Pages: 11624-11634
  • Peer Reviewed
• [Journal Article] The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2. (2008)
  • Author(s): Tanaka M., Sata T., Kawaguchi Y.
  • Journal Title: Virology Journal (5) Pages: 1-13
• [Journal Article] Identification of a Physiological Phosphorylation Site of the Herpes Simplex Virus 1-Encoded Protein Kinase Us3 Which Regulates Its Optimal Catalytic Activity In Vitro and Influences Its Function in Infected Cells. (2008)
  • Author(s): Kato A., Tanaka M., Yamamoto M., Asai R., Sata T., Nishiyama Y., Kawaguchi Y.
  • Journal Title: Jounal of Virology (82) Pages: 6172-6189
• [Journal Article] Simultaneous Tracking of Capsid, Tegument, and Envelope Protein Localization in Living Cells Infected with Triply Fluorescent Herpes Simplex Virus 1. (2008)
  • Author(s): Sugimoto K., Uema M., Sagara H., Tanaka M., Sata T., Hashimoto Y., Kawaguchi Y.
  • Journal Title: Jounal of Virology (82) Pages: 5198-5211
• [Journal Article] The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2. (2008)
  • Author(s): Tanaka M., Sata T., and Kawaguchi Y.
  • Journal Title: Virology Journal 5 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Identification of a Physiological Phosphorylation Site of the Herpes Simplex Virus 1-Encoded Protein Kinase Us3 Which Regulates Its Optimal Catalytic Activity In Vitro and Influences Its Function in Infected Cells. (2008)
  • Author(s): Kato A., Tanaka M., Yamamoto M., Asai R., Sata T., Nishiyama Y., and Kawaguchi Y.
  • Journal Title: Jounal of Virology 82 Pages: 6172-6189
  • Peer Reviewed
• [Journal Article] Simultaneous Tracking of Capsid, Tegument, and Envelope Protein Locali zation in Living Cells Infected with Triply Fluorescent Herpes Simplex Virus 1. (2008)
  • Author(s): Sugimoto K., Uema M., Sagara H., Tanaka M., Sata T., Hashimoto Y., and Kawaguchi Y.
  • Journal Title: Jounal of Virology 82 Pages: 5198-5211
  • Peer Reviewed
• [Presentation] HSV-1 VP22の新規機能の解析 (2010)
  • Author(s): 田中道子
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-09
• [Presentation] HSV-1 VP22の新規機能の解析 (2009)
  • Author(s): 田中道子
  • Organizer: 日本ウイルス学会
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 組換え単純ヘルペスウイルス,大腸菌、及びこれらを得る方法 (2002)
  • Inventor(s): 川口寧、田中道子、山梨祐司、香川弘之
  • Industrial Property Rights Holder: 川口寧、田中道子、山梨祐司、香川弘之
  • Industrial Property Number: 2002-273215
  • Filing Date: 2002-09-19