Regulation of autoimmunity by B lymphocyte coreceptor CD72

• Project/Area Number: 20790371
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: WATANABE Kozo Tokyo Medical and Dental University, 難治疾患研究所, 助教 (20376718)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 免疫寛容 / 自己免疫 / 免疫寛容・自己免疫 / CD72 / Cタイプ様レクチン / Bリンパ球 / 糖鎖リガンド

Research Abstract

In this study, I suggested that CD72 interacts with glycan by glycan array analysis and surface plasmon resonance analysis using purified recombinant CD72 protein. I also suggested that CD72 negatively regulates B cell proliferation by B cell antigen receptor ligation in the presence of CD72 glycan ligand by the experiments using purified B cells from CD72 defficient mice. These results suggest a new mechanism of negative regulation of a B cell immune response by the CD72 glycan ligand-CD72

Research Products

• [Journal Article] CD22-Antagonists with nanomolar potency : The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold (2011)
  • Author(s): Hajjaj H.M. Abdu-Allah, Kozo Watanabe, Gladys C. Completo, Magesh Sadagopan, Koji Hayashizaki, Chiaki Takaku, Taichi Tamanaka, Hiromu Takematsu, Yasunori Kozutsumi, James C. Paulson, Takeshi Tsubata, Hiromune Ando, Hideharu Ishida, Makoto Kiso
  • Journal Title: Bioorg.Med.Chem. 19 Pages: 1966-1971
• [Journal Article] CD22-Antagonists with nanomolar potency : The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold (2011)
  • Author(s): Hajjaj H.M.Abdu-Allah, Kozo Watanabe, Gladys C.Completo, Magesh Sadagopan, Koji Hayashizaki, Chiaki Takaku, Taichi Tamanaka, Hiromu Takematsu, Yasunori Kozutsumi, James C.Paulson, Takeshi Tsubata, Hiromune Ando, Hideharu Ishida, Makoto Kiso
  • Journal Title: Bioorg.Med.Chem. Volume: 19 Pages: 1966-71
  • Peer Reviewed
• [Journal Article] Potent Small Molecule Mouse CD22 Inhibitors: Exploring the Interaction of the Residue at C-2 of Sialic Acid Scaffold Bioorg. (2009)
  • Author(s): Abdu-Allah, H.H.M., Watanabe, K., Hayashizaki, K., Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H., Kiso, M.
  • Journal Title: Med.Chem.Lett. 19 Pages: 5573-5575
• [Journal Article] Synthesis of biotinylated sialoside to probe CD22-ligand interactions (2009)
  • Author(s): Abdu-Allah, H.H.M., Watanabe, K., Hayashizaki, K., Iwayama, Y., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H., Kiso, M.
  • Journal Title: Tetrahedron Lett. 50 Pages: 4488-4491
• [Journal Article] Autophagy connects antigen receptor signaling (2009)
  • Author(s): Watanabe, K., Tsubata, T.
  • Journal Title: Autophagy 5 Pages: 108-110
• [Journal Article] Potent Small Molecule Mouse CD22 Inhibitors : Exploring the Interaction of the Residue at C-2 of Sialic Acid Scaffold (2009)
  • Author(s): Abdu-Allah, H.H.M., Watanabe, K., Hayashizaki, K, Takaku, C., Tamanaka, T., Takematsu, H, Kozutsumi, Y., Tsubata, T., Ishida, H., Kiso, M.
  • Journal Title: Bioorg.Med.Chem.Lett Volume: 19 Pages: 5573-5
  • Peer Reviewed
• [Journal Article] Synthesis of biotinylated sialoside to probe CD22-ligand interactions (2009)
  • Author(s): Abdu-Allah, H.H.M., Watanabe, K., Hayashizaki, K., Iwayama, Y., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H., Kiso, M.
  • Journal Title: Tetrahedron Lett. Volume: 50 Pages: 5573-5
  • Peer Reviewed
• [Journal Article] Autophagy connects antigen receptor signaling to costimulatory signaling in B lymphocytes (2009)
  • Author(s): Watanabe K and Tsubata T
  • Journal Title: Autophagy 5 Pages: 108-110
  • Peer Reviewed
• [Journal Article] Induction of autophagy by B cell antigen receptor stimulation and its inhibition by costimulation (2008)
  • Author(s): Watanabe, K., Ichinose, S., Hayashizaki, K., Tsubata,T.
  • Journal Title: Biochem Biophys Res Commun. 374(2) Pages: 274-281
• [Journal Article] Induction of autophagy by B cell antigen receptor stimulation and its inhibition by costimulation (2008)
  • Author(s): Watanabe K, Ichinose S, Hayashizaki K and Tsubata T
  • Journal Title: Biochem Biophys Res Coommun 374 Pages: 274-281
  • Peer Reviewed
• [Journal Article] The use of cationic nanogels to deliver proteins to myeloma cells and primary T lymphocytes that poorly express heparan sulfate
  • Author(s): Kozo Watanabe, Yumiko Tsuchiya, Yoshinori Kawaguchi, Shin-ichi Sawada, Hirohito Ayame, Kazunari Akiyoshi, Takeshi Tsubata
  • Journal Title: Biomaterials. in press
• [Presentation] 合成ビオチン化CD22リガンドを用いた競合阻害ELISAによる新規合成CD22リガンドの活性評価 (2009)
  • Author(s): 渡辺幸造、竹松弘、鍔田武志
  • Organizer: 日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] CHP-NH2ナノゲルを用いたタンパク導入によるがん細胞とT細胞の細胞死制御 (2009)
  • Author(s): 渡辺幸造、土屋祐美子、河口佳典、菖蒲弘人、澤田晋一、秋吉一成、鍔田武志
  • Organizer: ケミカルバイオロジー学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-05-19
• [Presentation] The C-type lectin-like domain-containing B lymphocyte membrane molecule CD72 carries a Ca2+-independent (2009)
  • Author(s): Kozo Watanabe, Kenichi Nakajima, Yuka Imai, Motohiko Sato, Koji Hayashizaki, Takahiro Adachi, Kiichiro Totani, Yukishige Ito, Takeshi Tsubata
  • Organizer: lectin activity to oligomannose CREST国際シンポジウム
  • Place of Presentation: 千葉
• [Presentation] CHP-NH2 ナノゲルを用いたタンパク導入によるがん細胞とT細胞の細胞死制御 (2009)
  • Author(s): 渡辺幸造、土屋祐美子、河口佳典、菖蒲弘人、澤田晋一、秋吉一成、鍔田武志
  • Organizer: ケミカルバイオロジー学会
  • Place of Presentation: 神戸
• [Presentation] 合成ビオチン化CD22リガンドを用いた競合阻害ELISAによる新規合成CD22リガンドの活性評価 (2009)
  • Author(s): 渡辺幸造、竹松弘、鍔田武志
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 大阪
• [Presentation] Ca2+-independent lectin activity of C-type lectin-like domain-containing B lymphocyte membrane molecule CD72 for oligomannose (2008)
  • Author(s): Kozo Watanabe, Kenichi Nakajima Youka Imai, Takahiro Adachi,Motohiko Sato, Koji Hayashizaki, Kiichiro Totani, Yukishige Ito and Takeshi Tsubata
  • Organizer: 日本分子生物学会・生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] The C-type lectin-like domain-containing B lymphocyte membrane molecule CD72 carries a Ca2+-independent lectin activity to oligomannose (2008)
  • Author(s): Watanabe Kozo, Adachi Takahiro and Tsubata Takeshi
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] B細胞特異的C型レクチン様分子CD72のオリゴマンノースに対するCa2+非依存性のレクチン活性 (2008)
  • Author(s): 渡辺幸造, 安達貴弘, 鍔田武志
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 京都
• [Presentation] B細胞特異的C型レクチン様分子CD72はオリゴマンノースに対してCa2+非依存性のレクチン活性を有する (2008)
  • Author(s): 渡辺幸造, 中島権一, 今井有香, 安達貴弘,佐藤元彦, 林崎浩史, 戸谷希一郎, 伊藤幸成, 鍔田武志
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Book] Self-and non-self recognition by coreceptors on B lymphocytes : Regulation of B lymphocytes by CD19, CD21, CD22 and CD72.(D Kitamura, editor.)(How the Immune System Recognizes Self and Nonself)Chapter 7 (2008)
  • Author(s): Watanabe, K., Tsubata,T
  • Publisher: Springer-Verlag Tokyo, Inc.