Molecular mechanism underlying Super Th1 cell induction

• Project/Area Number: 20790379
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Hyogo College of Medicine
• Principal Investigator: NAKAHIRA Masakiyo Hyogo College of Medicine, 医学部, 助教 (60454758)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 免疫学 / アレルギー・ぜんそく / シグナル伝達 / 発現制御 / 遺伝子発現制御

Research Abstract

In this project, I studied the mechanism underlying IL-13 production in Th1 cell-derived Super Th1 cell by focusing on the expression of Gata3 transcription factor. I first revealed that IL-13 production in Super Th1 cells is regulated at the level of transcription. I next demonstrated that Gata3 is induced in Th1 cells by costimulation of TCR, IL-2, and IL-18 in the presence of IL-4 and that Gata3 is necessary to activate Il13 transcription in Super Th1 cells. Moreover, ChIP assay verified that Gata3 binds to a CGRE motif on Il13 gene, which might induce Il13 expression in Super Th1 cells.

Research Products

• [Journal Article] Contribution of IL-18 to eosinophilic airway inflammation induced by immunization and challenge with Staphylococcus aureus proteins. (2010)
  • Author(s): Kuroda-Morimoto M, Tanaka H, Hayashi N, Nakahira M, Imai Y, Imamura M, Yasuda K, Yumikura-Futatsugi S, Matsui K, Nakashima T, Sugimura K, Tsutsui H, Sano H, Nakanishi K.
  • Journal Title: Int Immunol. (in press)
  • NAID: 10027580749
  • Peer Reviewed
• [Journal Article] Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells. (2009)
  • Author(s): Yoshimoto T, Yasuda K, Tanaka H, Nakahira M, Imai Y, Fujimori Y, Nakanishi K.
  • Journal Title: Nat Immunol. Vol.10No.7 Pages: 706-712
  • Peer Reviewed
• [Journal Article] Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells (2009)
  • Author(s): Yoshimoto, T.et al.
  • Journal Title: Nature Immunology 10 Pages: 706-712
  • Peer Reviewed
• [Journal Article] Basophils contribute to Th2-IgE response in vivo by interleukin-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells
  • Author(s): Yoshimoto, T., Yasuda, K., Tanaka, H., Nakahira, M., Imai, Y., Fujimori, Y., and Nakanishi, K.
  • Journal Title: Nature Immunology (in press)
  • Peer Reviewed
• [Presentation] Super Th1細胞におけるIL-13発現制御機構 (2009)
  • Author(s): 中平雅清、中西憲司
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-03
• [Presentation] Super Th1細胞におけるIL-13発現制御機構 (2009)
  • Author(s): 中平雅清、中西憲司
  • Organizer: 第39回 日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-03