Investigation of arthritis diagnostic marker from synovial fluids using selective proteomics based analysis

• Project/Area Number: 20790416
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Osaka Medical College
• Principal Investigator: MATSUMURA Yoko Osaka Medical College, 医学部, 助教 (80388256)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 臨床検査医学 / プロテオミクス / 関節炎 / シトルリン化タンパク / 関節リウマチ / 変形性関節症 / 質量分析

Research Abstract

In this study, we identified three proteins, citrullinated fibrinogen, citrullinated fibronectin, and citrullinated vimentin in synovial fluids derived from RA patients by proteomics based analysis. Besides, compared to synovial fluids from OA patients, α1acid glycoprotein and fibrinogen were found in higher levels and apolipoprotein A-I was found in lower level in synovial fluids from RA patients. It was thought that there was a possibility of a new arthritis diagnostic marker by combination of these proteins.

Research Products

• [Journal Article] Detection of citrullinated proteins in synovi al fluids derived from patients with rheumat oid arthritis by proteomics-based analysis. (2008)
  • Author(s): Tabushi Y, Nakanishi T, Takeuchi T, Nakajima M, Ueda K, Kotani T, Makino S, Shimizu A, Hanafusa T, Takubo T.
  • Journal Title: Ann Clin Biochem. 45(Pt4) Pages: 413-7
  • Peer Reviewed
• [Presentation] 関節炎診断マーカーの同定 (2009)
  • Author(s): 松村洋子武内徹 中西豊文田窪孝行
  • Organizer: 第56回日本臨床検査医学会学術集会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2009-08-28
• [Presentation] 関節炎診断マーカーの同定-シトルリン化タンパクを用いてー (2009)
  • Author(s): 松村洋子
  • Organizer: 第56回 日本臨床検査医学会学術総会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2009-08-28
• [Presentation] 関節液中のシトルリン化タンパク解析による関節炎診断マーカーの検索 (2008)
  • Author(s): 田伏洋子武内徹中西豊文田窪孝行
  • Organizer: 第55回日本臨床検査医学会学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-11-30