The investigation into the endocytosis-mediated effects of the steroid hormones in renal proximal tubular epithelial cells

• Project/Area Number: 20790589
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: SATO Hiroyoshi Niigata University, 大学院・医歯学総合研究科, 特任助教 (30413610)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 腎臓学 / 近位尿細管 / エンドサイトーシス / ホルモン / メガリン / 物質再吸収 / ホルモン作用 / アンジオテンシンII / インスリン / シグナル伝達 / 腎臓内科

Research Abstract

I performed basic examination of possibility to have action through endocytosis of steroid hormone aldosterone etc in renal proximal tubular epithelial cells (PTEC), and I examined the expressional control of endocytotic receptor megalin by renin-angiotensin system (RAS) with a closely relation of aldosterone in PTEC. First, protein level of aldosterone receptor MR and aldosterone response gene SGK1 was confirmed in PTEC. Second, it was clarified the crosstalk with the insulin sinal that recovered the control of angiotensin II on the suppression of Megalin expression in PTEC.

Research Products

• [Journal Article] Molecular Mechanisms of receptor-mediated endocytosis in the renal proximal tubular epithelium (2010)
  • Author(s): 斎藤亮彦、佐藤博慶、飯野則昭、竹田徹朗
  • Journal Title: Journal of Biomedicine and Biotechnology 2010巻 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of receptor-mediated endocytosis in the renal proximal tubular epithelium (2010)
  • Author(s): Saito A, Sato H, Iino N, Takeda T
  • Journal Title: Journal of Biomedicine and Biotechnology 2010 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Megalin and nonmuscle myosin heavy chain IIA interact with the adaptor protein Disabled-2 in proximal tubule cells (2009)
  • Author(s): 保坂聖子、竹田徹朗、飯野則昭、細島康宏、佐藤博慶、忰田亮平、山本佳子、小林麻子、下条文武、斎藤亮彦
  • Journal Title: Kidney International 75巻 Pages: 1308-1315
  • Peer Reviewed
• [Journal Article] Regulation of megalin expression in cultured proximal tubule cells by angiotensin II type 1A receptor- and insulin- mediated signaling cross talk (2009)
  • Author(s): 細島康宏、佐藤博慶、山本佳子、忰田亮平、相馬多恵子、小林麻子、鈴木哲世、蒲澤秀門、竹山綾、生山健児、飯野則昭、西山成、Thomas J Thekkumkara、竹田徹朗、鈴木芳樹、下条文武、斎藤亮彦
  • Journal Title: Endocrinology 150巻 Pages: 871-878
  • Peer Reviewed
• [Journal Article] Regulation of megalin expression in cultured proximal tubule cells by angiotensin II type 1A receptor and insulin-mediated signaling cross talk (2009)
  • Author(s): Hosojima M, Sato H, 他15名
  • Journal Title: Endocrinology 150 Pages: 871-878
  • Peer Reviewed
• [Journal Article] Megalin and nonmuscle myosin heavy chain IIA interact with the adaptor protein Disabled-2 in proximal tubule cells (2009)
  • Author(s): Hosaka K, 他9名
  • Journal Title: Kidney International (in press)
  • Peer Reviewed
• [Presentation] 培養近位尿細管上皮細胞におけるGlucose, Insulin, Ang IIによるMegalinの発現調節機構 (2009)
  • Author(s): 佐藤博慶、山本佳子、細島康宏、相馬多恵子、鈴木哲世、蒲澤秀門、竹山綾、笹川泰司、生山健児、塩田友也、飯野則昭、竹田徹朗、鈴木芳樹、下条文武、斎藤亮彦
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-04
• [Presentation] 培養近位尿細管上皮細胞におけるGlucose, Insulin, Ang IIによるMegalinの発現調節機構 (2009)
  • Author(s): 佐藤博慶, ら(他14名)
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2009-06-04
• [Presentation] 近位尿細管エンドサイトーシス受容体メガリンの発現調節におけるアンジオテンシンII / AT1受容体とインスリン/グルコースのクロストーク (2008)
  • Author(s): 佐藤博慶、細島康宏、山本佳子、鈴木哲世、相馬多恵子、小林麻子、蒲澤秀門、竹山綾、飯野則昭、竹田徹朗、西山成、鈴木芳樹、下条文武、斎藤亮彦
  • Organizer: トランスポーターワークショップin鶴岡
  • Place of Presentation: 鶴岡(山形)
  • Year and Date: 2008-11-15
• [Presentation] 近位尿細管エンドサイトーシス受容体メガリンの発現調節におけるアンジオテンシンII/ATI受容体とインスリン/グルコースのクロストク (2008)
  • Author(s): 佐藤博慶、 他13名
  • Organizer: トランスポーターワークショップin鶴岡(トランスポーター研究会)
  • Place of Presentation: 東北公益文科大学鶴岡タウンキャンパス大学院ホール(山形県鶴岡市)
  • Year and Date: 2008-11-15
• [Presentation] Angiotensin II and insulin-mediated signaling pathways that regulate the expression of megalin in cultured proximal tubule cells (2008)
  • Author(s): 細島康宏、佐藤博慶、飯野則昭、小林麻子、相馬多恵子、竹田徹朗、西山成、Thomas J Thekkumkara、鈴木芳樹、下条文武、斎藤亮彦
  • Organizer: ISN Nexus symposium "Diabetes and the Kidney"
  • Place of Presentation: Dublin (Ireland)
  • Year and Date: 2008-06-27
• [Presentation] Angiotensin II and insulin-mediated signaling pathways that regulate the expression of megalin in cultured proximal tubule cells (2008)
  • Author(s): Hosojima M, 他10名
  • Organizer: ISN Nexus Symposium "Diabetes and the kidney" (International Society of Nephrology)
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2008-06-27