Identification of the origin of prion infection

• Project/Area Number: 20790608
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Tohoku University
• Principal Investigator: KOBAYASHI Atsushi Tohoku University, 大学院・医学系研究科, 助教 (50431507)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経病態生化学 / プリオン / クロイツフェルト・ヤコブ病 / 神経変性疾患 / 脳

Research Abstract

We previously reported an atypical sCJD-MM1 case (MM1-PL) presenting many plaque-type PrP deposits in the brain. To investigate the origin of the MM1-PL, we performed intracerebral inoculation of a brain homogenate from the MM1-PL patient into humanized mice with 129M/M or 129V/V genotype. The present our study shows that the MM1-PL was caused by the iatrogenic transmission of sCJD-VV2 prions.

Research Products

• [Journal Article] Experimental Verification of a Traceback Phenomenon in Prion Infection. (2010)
  • Author(s): Kobayashi A, Sakuma N, Matsuura Y, Mohri S, Aguzzi A, Kitamoto T.
  • Journal Title: J Virol. 84 Pages: 3230-3238
  • Peer Reviewed
• [Journal Article] Experimental verification of a traceback phenomenon in prion infection (2010)
  • Author(s): Kobayashi A
  • Journal Title: J Virol 84 Pages: 3230-3238
  • Peer Reviewed
• [Journal Article] Amino acid conditions near the GPI anchor attachment site of prion protein for the conversion and the GPI anchoring (2010)
  • Author(s): Hizume M
  • Journal Title: Biochem Biophys Res Commun 391 Pages: 1681-1686
  • Peer Reviewed
• [Journal Article] Amino acid conditions near the GPI anchor attachment site of prion protein for the conversion and the GPI anchoring. (2009)
  • Author(s): Hizume M, Kobayashi A, Mizusawa H, Kitamoto T.
  • Journal Title: Biochem Biophys Res Commun. 391 Pages: 1681-1686
  • Peer Reviewed
• [Journal Article] A traceback phenomenon can reveal the origin of prion infection. (2009)
  • Author(s): Kobayashi A, Asano M, Mohri S, Kitamoto T.
  • Journal Title: Neuropathology 29 Pages: 619-624
  • NAID: 10028192468
  • Peer Reviewed
• [Journal Article] The number of octapeptide repeat affects the expression and conversion of prion protein. (2009)
  • Author(s): Hiraga C, Kobayashi A, Kitamoto T.
  • Journal Title: Biochem Biophys Res Commun. 382 Pages: 715-719
  • Peer Reviewed
• [Journal Article] A traceback phenomenon can reveal the origin of prion infection (2009)
  • Author(s): Kobayashi A
  • Journal Title: Neuropathology 29 Pages: 619-624
  • NAID: 10028192468
  • Peer Reviewed
• [Journal Article] Human prion protein (PrP) 219K is converted to PrPsc but shows heter ozveous inhibition in variant Creutzfeldt-Jakob disease infection (2009)
  • Author(s): Hizume M
  • Journal Title: J Biol Chem 284 Pages: 3603-3609
  • Peer Reviewed
• [Journal Article] Thr but Asn of the N-glycosylation sites of PrP is indispensable for its misfolding (2008)
  • Author(s): Ikeda S
  • Journal Title: Biochem Biophys Res Commun 369 Pages: 1195-1198
  • Peer Reviewed
• [Journal Article] Plaque-type deposition of prion protein in the damaged white matter of sporadic Creutzfeldt-Jakob disease MM1 patients (2008)
  • Author(s): Kobayashi A
  • Journal Title: Acta Neuropathol 116 Pages: 561-566
  • Peer Reviewed
• [Presentation] ヒト異常型プリオン蛋白特異的抗体の作製 (2009)
  • Author(s): 小林篤史
  • Organizer: 2009年プリオン研究会
  • Place of Presentation: 宮城県
• [Remarks]
  • URL: http://www.prion.med.tohoku.ac.jp
• [Remarks]
  • URL: http://www.prion.med.tohoku.ac.jp
• [Remarks]
  • URL: http://www.prion.med.tohoku.ac.jp