Research for the localization of DJ-1 on the plasma membrane and functional roles of DJ-1 in membrane trafficking systems

• Project/Area Number: 20790626
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Juntendo University
• Principal Investigator: IMAMI Satoshi Juntendo University, 薬学部, 研究員 (80468579)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経科学 / 脳・神経 / 脳神経疾患 / DJ-1 / パーキンソン病 / マウス脳 / 神経細胞 / 細胞内局在 / 小胞体 / 細胞内膜輸送

Research Abstract

The aim of the present study is to investigate the precise subcellular distribution of endogenous DJ-1 in mouse brain and several cultured cell lines. In the present study, we found that DJ-1 was collected in the synaptic plasma membrane fractions together with integral membrane proteins. In agreement with this finding, double immunolabelling assay showed that DJ-1-immunoreactivity was highly detected in the membrane compartment of cultured cells and overlapped with integral membrane proteins on the cell surface. The findings strongly suggest that DJ-1 is associated with the plasma membrane in the mouse brain and cultured cells.

Research Products

• [Presentation] Possible localization of DJ-1 on the plasma membrane in the mouse brain and cultured cell lines (2008)
  • Author(s): Satoshi Imai
  • Organizer: 12th International congress of Parkinson' s disease and movement disorders
  • Place of Presentation: Hilton Chicago, Chicago, USA
• [Presentation] Possible association of DJ-1 with the synaptic membrane in the mouse brain (2008)
  • Author(s): 今井哲司
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム、東京
• [Presentation] 家族性パーキンソン病原因分子DJ-1の細胞膜および細胞内小胞体における局在に関する検討 (2008)
  • Author(s): 今井哲司
  • Organizer: 生体機能と創薬シンポジウム2008
  • Place of Presentation: 星薬科大学、東京
• [Presentation] DJ-1蛋白の細胞膜組織における局在に関する検討 (2008)
  • Author(s): 今井哲司
  • Organizer: 第2回Movement disorder society Japan
  • Place of Presentation: 国立京都国際会議場、京都
• [Presentation] Possible localization of DJ-1 on the plasma membrane in the mouse brain and cultured cell lines (2008)
  • Author(s): Satoshi Imai
  • Organizer: Neuroscience 2008
  • Place of Presentation: Renaissance Washington DC Hotel, Washington DC, USA
• [Presentation] Possible localization of DJ-1 on the plasma membrane in the mouse brain and cultured cell lines (2008)
  • Author(s): Satoshi Imai
  • Organizer: 12^<th> International congress of Parkinson' s disease and movement disorders
  • Place of Presentation: Hilton Chicago, Chicago, USA
• [Presentation] Possible association of DJ-1 with the synaptic membrane in the mousebrain (2008)
  • Author(s): 今井哲司
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 家族性パーキンソン病原因分子DJ-1の細胞膜および細胞内小胞体における局在に関する検 (2008)
  • Author(s): 今井哲司
  • Organizer: 生体機能と創薬シンポジウム2008
  • Place of Presentation: 星薬科大学(東京)
• [Presentation] DJ-1蛋白の細胞膜組織における局在に関する検討 (2008)
  • Author(s): 今井哲司
  • Organizer: 第2回Movement disorder society Japan
  • Place of Presentation: 国立京都国際会議場
• [Presentation] Possible localization of DJ-1 on the plasma membrane in the mouse brain and cultured cell lines (2008)
  • Author(s): Satoshi Imai
  • Organizer: Neuroscience 2008 (Society for Neuroscience)
  • Place of Presentation: ワシントンD.C(米国)