Establishment of the methods for regeneration of steroidogenic cells from mesencymal stem cells and analysis of its mechanism

• Project/Area Number: 20790661
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyushu University
• Principal Investigator: TANAKA Tomoko Kyushu University, 医学部, 研究員 (10380528)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 内分泌学 / ステロイドホルモン / 問葉系幹細胞 / 間葉系幹細胞

Research Abstract

Although we reported that Ad4BP/SF-1 transformed mesenchymal stem cells (MSCs) into steroidogenic cells, Ad4BP/SF-1-converted steroidogenic cells produce both adrenal-type steroids and gonad-type steroids. To clarify the underlying mechanism, we analyzed the expression profile and focused on the several factors up-regulated by Ad4BP/SF-1. Among these factors, Sfrp-1 and Inhbb might be related to Ad4BP/SF-1-mediated differentiation of MSCs into gonad-type steroidogenic cells.

Research Products

• [Journal Article] Dihydrotestosterone suppresses foam cell formation and attenuates atherosclerosis development (2010)
  • Author(s): Qiu Y, Yanase T, Hu H, Tanaka T, Nishi Y, Liu M, Sueishi K, Sawamura T, Nawata H
  • Journal Title: Endocrinology (in press)
  • Peer Reviewed
• [Journal Article] Dihydrotestosterone suppresses foam cell formation and attenuates atherosclerosis development (2010)
  • Author(s): Qiu et al
  • Journal Title: Endocrinology
  • Peer Reviewed
• [Journal Article] ステロイド産生細胞研究の現状 (2009)
  • Author(s): 柳瀬敏彦、田中智子
  • Journal Title: 日本生殖内分泌学会雑誌 14巻 Pages: 51-53
• [Journal Article] A novel synthetic androgen receptor ligand, S42, works as a selective androgen receptor modulator and possesses metabolic effects with little impact on the prostate (2009)
  • Author(s): Liu M, Yanase T, Tanaka T, Fan W, Nomura M, Kawate H, Okabe T, Takayanagi R, Nawata H
  • Journal Title: Endocrinology 12 Pages: 5606-16
  • Peer Reviewed
• [Journal Article] A novel synthetic androgen receptor ligand, S42, works as a selective androgen receptor modulator and possesses metabolic effects with little impact on the prostate (2009)
  • Author(s): 柳瀬敏彦 et al
  • Journal Title: Endocrinology 150 Pages: 5606-16
  • Peer Reviewed
• [Journal Article] ステロイド産生細胞研究の現状 (2009)
  • Author(s): 柳瀬敏彦
  • Journal Title: 日本生殖内分泌学会雑誌 14 Pages: 51-53
• [Journal Article] Methylation of a conserved intronic CpG island of mouse SF-1 is associated with cell-specific expression of SF-1 in a culture system but not with tissue-specific expression (2008)
  • Author(s): Shirouhzu H, Okabe T, Gondo S, Tanaka T, Ohe K, Morinaga H, Kawate H, Nomura M, Takayanagi R, Nawata H, Yanase T
  • Journal Title: Biochem Biophys Res Commun 369 Pages: 862-7
  • Peer Reviewed
• [Journal Article] Adipose tissue-derived and bone marrow-derived mesenchymal cells develop into different lineage of steroidogenic cells by forced expression of steroidogenic factor-1 (2008)
  • Author(s): Gondo S, Okabe T, Tanaka T, Morinaga H, Nomura M, Takayanagi R, Nawata H, Yanase T
  • Journal Title: Endocrinology 9 Pages: 4717-25
  • Peer Reviewed
• [Journal Article] Adipose tissue-derived and bone marrow-derived mesenchymal cells develop into different lineage of steroidogenic cells by forced expression of steroidogenic factor 1 (2008)
  • Author(s): S. Gondo, et.al.
  • Journal Title: Endocrinology 149 Pages: 4717-4725
  • Peer Reviewed
• [Journal Article] Methylation of a conserved intronic CpG island of mouse SF-1 is associ ated with cell-specific expression of SF-1 in a culture system but not with tissue-specific expression (2008)
  • Author(s): H. Sirohzu, et.al.
  • Journal Title: Biochemical and Biophysiacl Research Communica tions 369 Pages: 862-867
  • Peer Reviewed
• [Presentation] Regeneration of steroidogenic cells form mesenchymal stem cells (2010)
  • Author(s): Tomoko Tanaka
  • Organizer: 14th International Congress of Endocrinology Official Satellite Symposia
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-30
• [Presentation] Steroidogenic cells regenerated from mesenchymal stem cells (2010)
  • Author(s): 田中智子
  • Organizer: 14^<th> International Congress of Endocrinology Official Satellite Symposia
  • Place of Presentation: Kyoto international conference center
  • Year and Date: 2010-03-30
• [Presentation] Effects of Sfrp-1 and Inhibin beta B on SF-1 mediated differntiation of mesenchymal stem cells into steroidogenic cells (2010)
  • Author(s): Tomoko Tanaka
  • Organizer: 14th International Congress of Endocrinology
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-28
• [Presentation] Effects of Sfrp-1 and Inhibin beta B on SF-1 mediated differntiation of mesenchymal stem cells into steroidogenic cells (2010)
  • Author(s): 田中智子
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto international conference center
  • Year and Date: 2010-03-28
• [Presentation] 間葉系幹細胞におけるSF-1/Ad4BP誘導遺伝子のステロイド産生に及ぼす影響 (2009)
  • Author(s): 田中智子
  • Organizer: 第17回日本ステロイドホルモン学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-11-14
• [Presentation] 間葉系幹細胞におけるSF-1/Ad4BP誘導遺伝子のステロイド産生に及ぼす影響 (2009)
  • Author(s): 田中智子
  • Organizer: 第17回日本ステロイドホルモン学会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-11-14
• [Presentation] 間葉系幹細胞におけるSF-1/Ad4BP誘導遺伝子のステロイド産生に及ぼす影響 (2009)
  • Author(s): 田中智子
  • Organizer: アンドロロジー学会第28回学術大会
  • Place of Presentation: 富山
  • Year and Date: 2009-07-03
• [Presentation] 間葉系幹細胞におけるSF-1/Ad4BP誘導遺伝子のステロイド産生に及ぼす影響 (2009)
  • Author(s): 田中智子
  • Organizer: アンドロロジー学会第28回学術大会
  • Place of Presentation: ANAクラウンプラザホテル富山
  • Year and Date: 2009-07-03
• [Presentation] 間葉系幹細胞からのステロイド産生細胞への分化誘導における性分化関連因子の影響について (2008)
  • Author(s): 田中智子, 他
  • Organizer: 日本アンドロロジー学会第27回学術大会
  • Place of Presentation: 京都
  • Year and Date: 2008-07-05
• [Presentation] 間葉系幹細胞からのステロイド産生細胞への分化誘導における各種性分化関連因子の関与について (2008)
  • Author(s): 田中智子, 他
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森
  • Year and Date: 2008-05-16
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/interna5/index.html