Cancer stem cell theory in thyroid carcinogenesis

• Project/Area Number: 20790662
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Nagasaki University
• Principal Investigator: MITSUTAKE Norisato Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (50404215)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 甲状腺 / 幹細胞

Research Abstract

We have successfully developed a novel culture system to isolate stem-like cells from human normal thyroid tissues. These cells had differentiation potentials not only into thyroid follicular cells but also into neuron or adipocytes. The isolated cells may be directly reprogrammed from thyroid follicular cells, and this reprogramming might be involved in generating cancer stem cells during carcinogenesis.

Research Products

• [Journal Article] Fibroblast-mediated in vivo and in vitro growth promotion of tumorigenic rat thyroid carcinoma cells but not normal Fisher rat thyroid follicular cells. (2009)
  • Author(s): Saitoh O, Mitsutake N, Nakayama T, Nagayama Y
  • Journal Title: Thyroid 19 Pages: 735-742
  • Peer Reviewed
• [Journal Article] Lack of GNAQ hotspot mutation in papillary thyroid carcinomas. (2009)
  • Author(s): Matsuse M, Mitsutake N, Nishihara E, Rogounovitch T, Saenko V, Rumyantsev P, Lushnikov E, Suzuki K, Miyauchi A, Yamashita S
  • Journal Title: Thyroid 19 Pages: 921-922
  • Peer Reviewed
• [Journal Article] 甲状腺癌における癌遺伝子の役割と分子標的治療 (2009)
  • Author(s): 光武範吏、山下俊一
  • Journal Title: 日本内科学会雑誌 98 Pages: 1999-2005
  • NAID: 10025193476
• [Journal Article] Mutation analysis of RAP1 gene in papillary thyroid carcinomas. (2009)
  • Author(s): Matsuse M, Mitsutake N, Rogounovitch T, Saenko V, Nakazawa Y, Rumyantsev P, Lushnikov E, Suzuki K, Yamashita S
  • Journal Title: Endocr J 56 Pages: 161-164
  • NAID: 10025610615
  • Peer Reviewed
• [Journal Article] Fagin JA 2009 Endogenous expression of Hras(G12V) induces developmental defects and neoplasms with copy number imbalances of the oncogene. (2009)
  • Author(s): *Chen X, *Mitsutake N, LaPerle K, Akeno N, Zanzonico P, Longo VA, Mitsutake S, Kimura ET, Geiger H, Santos E, Wendel HG, Franco A, Knauf JA
  • Journal Title: Proc Natl Acad Sci U S A 106 Pages: 7979-7984
  • Peer Reviewed
• [Journal Article] Polymorphisms of DNA damage response genes in radiation-related and sporadic papillary thyroid carcinoma. (2009)
  • Author(s): Akulevich NM, Saenko VA, Rogounovitch TI, Drozd VM, Lushnikov EF, Ivanov VK, Mitsutake N, Kominami R, Yamashita S
  • Journal Title: Endocr Relat Cancer 16 Pages: 491-503
  • NAID: 120006984014
  • Peer Reviewed
• [Journal Article] Fibroblast-mediated in vivo and in vitro growth promotion of tumorigenic rat thyroid carcinoma cells but not normal Fisher rat thyroid follicular cells (2009)
  • Author(s): Saitoh O
  • Journal Title: Thyroid 19 Pages: 735-742
  • Peer Reviewed
• [Journal Article] Lack of GNAQ hotspot mutation in papillary thyroid carcinomas (2009)
  • Author(s): Matsuse M
  • Journal Title: Thyroid 19 Pages: 921-922
  • Peer Reviewed
• [Journal Article] Endogenous expression of Hras (G12V) induces developmental defects and neoplasms with copy number imbalances fo the oncogene (2009)
  • Author(s): Chen X, Mitsutake N
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 7979-7984
  • Peer Reviewed
• [Journal Article] Polymorphisms of DNA damage response genes in radiation-related and sporadic papillary thyroid carcinoma (2009)
  • Author(s): Akulevich N
  • Journal Title: Endocr Relat Cancer 16 Pages: 491-503
  • Peer Reviewed
• [Journal Article] Mutation analysis of RAP1 gene in papillary thyroid carcinomas (2009)
  • Author(s): Matsuse M
  • Journal Title: Endocr J 56 Pages: 161-164
  • Peer Reviewed
• [Journal Article] Reciprocal paracrine interactions between normal human epithelial and mesenchymal cells protect cellular DNA from radiation-induced damage (2009)
  • Author(s): Nakazawa Y
  • Journal Title: Int J Radiat Oncol Biol Phys 71 Pages: 567-577
  • Peer Reviewed
• [Journal Article] Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells. (2008)
  • Author(s): Takakura S, Mitsutake N, Nakashima M, Namba H, Saenko VA, Rogounovitch TI, Nakazawa Y, Hayashi T, Ohtsuru A, Yamashita S
  • Journal Title: Cancer Sci 99 Pages: 1147-1154
  • NAID: 10024007190
  • Peer Reviewed
• [Journal Article] Reciprocal paracrine interactions between normal human epithelial and mesenchymal cells protect cellular DNA from radiation-induced damage. (2008)
  • Author(s): Nakazawa Y, Saenko V, Rogounovitch T, Suzuki K, Mitsutake N, Matsuse M, Yamashita S
  • Journal Title: Int J Radiat Oncol Biol Phys 71 Pages: 567-577
  • Peer Reviewed
• [Journal Article] Dehydroxymethylepoxyquinomicin, a novel nuclear Factor-kappaB inhibitor, enhances antitumor activity of taxanes in anaplastic thyroid cancer cells. (2008)
  • Author(s): Meng Z, Mitsutake N, Nakashima M, Starenki D, Matsuse M, Takakura S, Namba H, Saenko V, Umezawa K, Ohtsuru A, Yamashita S
  • Journal Title: Endocrinology 149 Pages: 5357-5365
  • NAID: 120006982281
  • Peer Reviewed
• [Journal Article] Dehydroxymethylepoxyquinomicin, a novel nuclear factor-kappaB inhibitor, enhances antitumor activity of taxanes in anaplastic thyroid cancer cells (2008)
  • Author(s): Meng Z
  • Journal Title: Endocrinology 149 Pages: 5357-5365
  • Peer Reviewed
• [Journal Article] Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells (2008)
  • Author(s): Takakura S
  • Journal Title: Cancer Sci 99 Pages: 1147-1154
  • Peer Reviewed
• [Presentation] A mechanism of molecular diversity of monoclonal papillary thyroid carcinomas (2009)
  • Author(s): Rogounovitch T
  • Organizer: 日本甲状腺学会
  • Place of Presentation: 名古屋
• [Presentation] 甲状腺乳頭癌におけるGNAQ遺伝子変異 (2009)
  • Author(s): 松瀬美智子
  • Organizer: 日本甲状腺学会
  • Place of Presentation: 名古屋
• [Presentation] ヒト甲状腺正常幹細胞単離培養の試み (2009)
  • Author(s): 光武範吏
  • Organizer: 日本甲状腺学会
  • Place of Presentation: 名古屋
• [Presentation] Mutational and clinico-pathological analysis of papillary thyroid carcinoma in Serbian patients Asia and Oceania (2009)
  • Author(s): Stanojevic B
  • Organizer: Thyroid Association Congress
  • Place of Presentation: Nagoya
• [Presentation] Molecular diversity of papillary thyroid carcinoma assessed by simultaneous determination of tumor clonality and somatic oncogenic mutation (2009)
  • Author(s): Saenko V
  • Organizer: Asia and Oceania Thyroid Association Congress
  • Place of Presentation: Nagoya
• [Presentation] Efficient preoperative evaluation of thyroid nodules by molecular analysis of FNAB materials (2009)
  • Author(s): Saenko V
  • Organizer: 日本内分泌学会
  • Place of Presentation: 前橋
• [Presentation] Clinical course of papillary thyroid carcinoma in children and adolescent age groups (2009)
  • Author(s): Rumyantsev P
  • Organizer: 日本内分泌学会
  • Place of Presentation: 前橋
• [Presentation] 難治性甲状腺癌に対する分子標的治療・放射線併用療法 (2009)
  • Author(s): 熊谷敦史
  • Organizer: 日本内分泌学会
  • Place of Presentation: 前橋
• [Presentation] 甲状腺細胞におけるBRAFの機能 (2009)
  • Author(s): 光武範吏
  • Organizer: 日本内分泌学会
  • Place of Presentation: 前橋
• [Presentation] Molecular diversity of papillary thyroid carcinoma assessed by simultaneous determination of tumor clonality and somatic oncogenic mutation (2009)
  • Author(s): Saenko V
  • Organizer: 9^<th> Asia and Oceania Thyroid Association Congress
  • Place of Presentation: Nagoya
• [Presentation] Mutational and clinico-pathological analysis of papillary thyroid carcinoma in Serbian patients (2009)
  • Author(s): Stanojevic B
  • Organizer: 9^<th> Asia and Oceania Thyroid Association Congress
  • Place of Presentation: Nagoya
• [Presentation] 甲状腺細胞におけるBRAFの機能 (2009)
  • Author(s): 光武範吏
  • Organizer: 第82回日本内分泌学会総会
  • Place of Presentation: 前橋
• [Presentation] 難治性甲状腺癌に対する分子標的治療・放射線併用療法 (2009)
  • Author(s): 熊谷敦史
  • Organizer: 第82回日本内分泌学会総会
  • Place of Presentation: 前橋
• [Presentation] Clinical course of papillary thyroid carcinoma in children and adolescent age groups (2009)
  • Author(s): Rumyantsev P
  • Organizer: 第83回日本内分泌学会総会
  • Place of Presentation: 前橋
• [Presentation] Efficient preoperative evaluation of thyroid nodules by molecular analysis of FNAB materials (2009)
  • Author(s): Saenko V
  • Organizer: 第84回日本内分泌学会総会
  • Place of Presentation: 前橋
• [Presentation] ヒト甲状腺正常幹細胞単離培養の試み (2009)
  • Author(s): 光武範吏
  • Organizer: 第52回日本甲状腺学会
  • Place of Presentation: 名古屋
• [Presentation] 甲状腺乳頭癌におけるGNAQ遺伝子変異 (2009)
  • Author(s): 松瀬美智子
  • Organizer: 第52回日本甲状腺学会
  • Place of Presentation: 名古屋
• [Presentation] A mechanism of molecular diversity of monoclonal papillary thyroid carcinomas (2009)
  • Author(s): Rogounovitch T
  • Organizer: 第52回日本甲状腺学会
  • Place of Presentation: 名古屋
• [Presentation] 甲状腺乳頭癌におけるRAP1遺伝子変異 (2008)
  • Author(s): 松瀬美智子
  • Organizer: 日本甲状腺学会
  • Place of Presentation: 宇都宮
• [Presentation] DHMEQ, a novel NF-kappaB inhibitor (2008)
  • Author(s): Mitsutake N DHMEQ
  • Organizer: enhances anti-tumor activity of taxanes in anaplastic thyroid cancer cells International Congress of Endocrinology
  • Place of Presentation: Rio de Janeiro, Brazil
• [Presentation] 甲状腺未分化癌細胞に対するNF-kappaB阻害剤DHMEQとタキサンの併用効果の検討 (2008)
  • Author(s): 光武範吏
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] Molecular and clinico-pathological analysis of pediatric thyroid cancers in Belarus Endocrine (2008)
  • Author(s): Rogounovitch T, Mitsutake N
  • Organizer: Society Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] 甲状腺未分化癌に対する抗癌剤と分子標的治療の併用効果 (2008)
  • Author(s): 光武範吏
  • Organizer: 日本内分泌学会
  • Place of Presentation: 青森
• [Presentation] DHMEQ, a novel NF-kappaB inhibitor, enhances anti-tumor activity of taxanes in anaplastic thyroid cancer cells (2008)
  • Author(s): Mitsutake N
  • Organizer: International Congress of Endocrinology 2008
  • Place of Presentation: Rio de Janeiro, Brazil
• [Presentation] Molecular and clinico-pathological analysis of pediatric thyroid cancers in Belarus (2008)
  • Author(s): Rogounovitch T
  • Organizer: Endocrine Society Meeting 2008
  • Place of Presentation: San Francisco, USA
• [Presentation] 甲状腺未分化癌に対する抗癌剤と分子標的治療の併用効果 (2008)
  • Author(s): 光武範吏
  • Organizer: 第81回日本内分泌学会総会
  • Place of Presentation: 青森
• [Presentation] 甲状腺未分化癌細胞に対するNF-kapPaB阻害剤DHMEQとタキサンの併用効果の検討 (2008)
  • Author(s): 光武範吏
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 甲状腺乳頭癌におけるRAP1遺伝子変異 (2008)
  • Author(s): 松瀬美智子
  • Organizer: 第51回日本甲状腺学会
  • Place of Presentation: 宇都宮