Analysis of functional regulatory mechanism of human hematopoietic stem cell by cyclin C

• Project/Area Number: 20790672
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Nagoya University
• Principal Investigator: MIYATA Yasuhiko Nagoya University, 医学部・附属病院, 医員 (40467303)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 造血幹細胞 / 細胞周期 / サイクリンC / 静止期 / 生着 / cyclin C

Research Abstract

We have identified an important role of cyclin C (CCNC) in regulating human hematopoietic stem/progenitor cell (HSPC) quiescence, as knocking down CCNC expression in human cord blood CD34+ cells resulted in a significant increase in quiescent cells that maintain CD34 expression. CCNC knockdown also promotes in vitro HSPC expansion and enhances their engraftment potential in sublethally irradiated immunode- ficient mice. Our studies establish cyclin C as a critical regulator of the G0/G1 transition of human HSPCs and suggest that modulating cyclin C levels may be useful for HSC expansion and more efficient engraftment.

Research Products

• [Journal Article] Cyclin C Regulates Human Hematopoietic Stem/Progenitor Cell Quiescence. (2010)
  • Author(s): Miyata Y, Liu Y, Jankovic V, Sashida G, Lee JM, Shieh JH, Naoe T, Moore M, Nimer SD.
  • Journal Title: Stem Cells. Vol.28 Pages: 308-317
  • Peer Reviewed
• [Journal Article] Irrespective of CD34 expression, lineage-committed cell fraction reconstitutes and re-establishes transformed Philadelphia chromosome-positive leukemia in NOD / SCID / IL-2Rγc-/- mice. (2010)
  • Author(s): Tanizaki R, Nomura Y, Miyata Y, Minami Y, Abe A, Hanamura A, Sawa M, Murata M, Kiyoi H, Matsushita T, Naoe T
  • Journal Title: Cancer Science. 101 Pages: 631-8
  • NAID: 10026589230
  • Peer Reviewed
• [Journal Article] Cyclin C Regulates Human Hematopoietic Stem/Progenitor Cell Quiescence (2010)
  • Author(s): Yasuhiko Miyata
  • Journal Title: Stem Cells 28 Pages: 308-317
  • Peer Reviewed
• [Journal Article] Nimer SD. ELF4/MEF activates MDM2 expression and blocks oncogene-induced p16 activation to promote transformation. (2009)
  • Author(s): Sashida G, Liu Y, Elf S, Miyata Y, Ohyashiki K, Izumi M, Menendez S
  • Journal Title: Mol Cell Biol. 29(13) Pages: 3687-99
  • Peer Reviewed
• [Journal Article] The p53 tumor suppressor protein is a critical regulator of hematopoietic stem cell behavior. (2009)
  • Author(s): Liu Y, Elf SE, Asai T, Miyata Y, Liu Y, Sashida G, Huang G, Di Giandomenico S, Koff A, Nimer SD.
  • Journal Title: Cell Cycle. 8(19) Pages: 3120-4
• [Presentation] PAX5-PML Acts as a Dual Dominant-Negative Form of Both PAX5 and PML. (2009)
  • Author(s): 倉橋伸悟
  • Organizer: 51st American Society of Hematology Annual Meeting
  • Place of Presentation: ニューオーリンズ(米国)
  • Year and Date: 2009-12-05
• [Presentation] Akt-Mediated Phosphorylation of Bmi1 Regulates Its Chromatin Association and Growth Promoting Properties. (2009)
  • Author(s): Liu Yan
  • Organizer: 51st American Society of Hematology Annual Meeting
  • Place of Presentation: ニューオーリンズ(米国)
  • Year and Date: 2009-12-05
• [Presentation] Reversible expression of CD34 in Ph-positive human leukemia cells transplanted into NOG mice. (2009)
  • Author(s): 谷崎亮平
  • Organizer: 第71回日本血液学会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-10-26
• [Presentation] MAPキナーゼシグナル経路によるリンパ球系転写因子Pax5の機能調節 (2009)
  • Author(s): 早川文彦
  • Organizer: 第71回日本血液学会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-10-25
• [Presentation] Oncogenic fusion protein PAX5-PML acts as a dominant negative on both PAX5 and PML. (2009)
  • Author(s): 倉橋伸悟
  • Organizer: 第71回日本血液学会
  • Place of Presentation: 都国際会議場(京都)
  • Year and Date: 2009-10-24
• [Presentation] Cyclin C Regulates Human Hematopoietic Stem/Progenitor Cell Quiescence. (2009)
  • Author(s): 宮田泰彦
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー(東京)
  • Year and Date: 2009-05-16
• [Presentation] Cyclin C Regulates Quiescence of Human Hematopoietic Primitive Progenitor Cells (2009)
  • Author(s): 宮田泰彦
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー(東京)
  • Year and Date: 2009-05-16