Analysis of the drug sensitivity and the genetic alteration of melanoma

• Project/Area Number: 20790808
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Sapporo Medical University
• Principal Investigator: HIDA Tokimasa Sapporo Medical University, 医学部, 助教 (90464487)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 癌

Research Abstract

The purpose of this study was to establish a primary culture system of melanoma cells which derived from surgical samples of melanoma tissues, mainly from primary acral lentiginous melanoma. As a result, we obtained surgical samples from 19 patients, from which three lines of melanoma culture were obtained. Fresh frozen tissue samples were also collected from the same patients to examine melanoma-related genes and their expression. Hot spot mutations were not detected in N-ras, BRAF or c-kit. This system will allow us to examine the correlation between the behavior of melanoma cells, especially of their drug resistance, and their genetic profile, and to choose effective chemotherapeutic drugs for individual melanoma patients.

Research Products

• [Journal Article] Rab7 is a critical mediator in vesicular transport of tyrosinase-related protein 1 in melanocytes. (2011)
  • Author(s): Hida T, Sohma H, Kokai Y, Kawakami A, Hirosaki K, Okura M, Tosa N, Yamashita T, Jimbow K
  • Journal Title: J Dermatol 38 Pages: 432-441
  • Peer Reviewed
• [Journal Article] Cytokeratin expression patterns in multiple infundibulocystic basal cell carcinoma. (2011)
  • Author(s): Hida T, Saga K, Kimura T
  • Journal Title: J Cutan Pathol 38 Pages: 309-313
  • Peer Reviewed
• [Journal Article] Agouti protein, mahogunin, and attractin in pheomelanogenesis and melanoblast-like alteration of melanocytes : a cAMP-independent pathway. (2009)
  • Author(s): Hida T, Wakamatsu K, Sviderskaya EV, Donkin AJ, Montoliu L, Lynn Lamoreux M, Yu B, Millhauser GL, Ito S, Barsh GS, Jimbow K, Bennett DC
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 623-634
  • Peer Reviewed
• [Journal Article] Macrophage inhibitory cytokine-1 : a new player in melanoma development. (2009)
  • Author(s): Yamashita T, Yoneta A, Hida T
  • Journal Title: J Invest Dermatol 129(2) Pages: 262-264
  • Peer Reviewed
• [Journal Article] Spontaneous regression of bowenoid papulosis in a patient with acquired immunodeficiency syndrome after an increase in peripheral CD4+ T lymphocytes. (2009)
  • Author(s): Kawakami A, Saga K, Ono I, Hida T, Jimbow K, Yamashita T
  • Journal Title: Int J Dermatol 48 Pages: 210-212
  • Peer Reviewed
• [Presentation] Efficacy and safety of 5% imiquimod cream for the treatment of skin cancers. (2010)
  • Author(s): Yoneta A, Endo M, Mori S, Yanagisawa K, Hida T, Yamashita T
  • Organizer: The first eastern Asia Dermatology Congress
  • Place of Presentation: Fukuoka, Japan