| Project/Area Number |
20790808
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
HIDA Tokimasa Sapporo Medical University, 医学部, 助教 (90464487)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
|---|
| Keywords | 癌 |
|---|
| Research Abstract |
The purpose of this study was to establish a primary culture system of melanoma cells which derived from surgical samples of melanoma tissues, mainly from primary acral lentiginous melanoma. As a result, we obtained surgical samples from 19 patients, from which three lines of melanoma culture were obtained. Fresh frozen tissue samples were also collected from the same patients to examine melanoma-related genes and their expression. Hot spot mutations were not detected in N-ras, BRAF or c-kit. This system will allow us to examine the correlation between the behavior of melanoma cells, especially of their drug resistance, and their genetic profile, and to choose effective chemotherapeutic drugs for individual melanoma patients.
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