To search the novel miRNAs relatedwith immune rejection and tolerance after mice l iver transplantation

• Project/Area Number: 20790929
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: KITAZAWA Yusuke Osaka University, 医学部, 助教 (00467581)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 肝移植 / 免疫寛容 / 遺伝子 / mRNA / miRNA

Research Abstract

To specifically monitor and predict the alloimmune response and tolerance, We studied transcriptional patterns in mouse hepatic allografts, a unique spontaneous tolerance model by mRNA and miRNA array, and simultaneously confirmed results by real time quantitative RT-PCR. Furthermore, We assessed hlstologlcal changes of the allografts by HE staining. These results suggest that the expression profiles of the graft mRNA and miRNA may have the sensitivityand specificity to distinguish allograft rejection and tolerance.

Research Products

• [Journal Article] Prevention of graft-versus-host diseases by IJ7 Vl-vo supCD28mAb-expanded antigen-speclfi c nTreg cells (2010)
  • Author(s): KltazawaY, LIXK, LluZ, KimuraH, Isaka Y, Htlnig T, Takahara S.
  • Journal Title: Cell Transplant. (in press)
  • Peer Reviewed
• [Journal Article] PDl/B7-Hl lnteractlon contribute to the spontaneous acceptance of mouse liver allograft (2010)
  • Author(s): MoritaM, FujlnoM, JlangGP, KitazawaY, Xie L, Azuma M, Yagita H, Nagao S, Sugioka A, Kurosawa Y, Takahara S, Fung J, Qlan S, Lu L, LI X-K.
  • Journal Title: Am J Transplant 10(1) Pages: 40-46
  • Peer Reviewed
• [Journal Article] PD1/B7-H1 interaction contribute to the spontaneous acceptance of mouse liver allograft (2010)
  • Author(s): Morita M, et al
  • Journal Title: Am J Transplant 10(1) Pages: 40-46
  • Peer Reviewed
• [Journal Article] Superagonlst CD28 antibody preferentially expanded Foxp3-expressing nTreg cel ls and prevented graft-versus-host diseases. (2009)
  • Author(s): Kltazawa Y, Fujino M, Li XK, Xie L, Ichimaru N, Okuml M, Nonomura N, Tsujimura A, Isaka Y, Kimura H, mnlg T, Takahara S.
  • Journal Title: Cell Transplant. 18(5) Pages: 627-37
  • Peer Reviewed
• [Journal Article] Superagonist CD28 antibody preferentially expanded Foxp3-expressing nTreg cells and prevented graft-versus-host diseases (2009)
  • Author(s): Kitazawa Y, et al.
  • Journal Title: Cell Transplant. 18(5) Pages: 627-637
  • NAID: 110009675237
  • Peer Reviewed
• [Journal Article] Superagonist CD28 antibody preferentially expanded Foxp3-expressing nTreg cells and prevented graft-versus-host diseases (2009)
  • Author(s): Kitazawa Y, et.al.
  • Journal Title: Cell Transplantation (In press)
  • NAID: 110009675237
  • Peer Reviewed
• [Journal Article] Foxp3-expressing regulatory T cells expanded with CD28 superagonlst antibody can prevent rat cardiac allograft rejection. (2008)
  • Author(s): Kitazawa Y, Fujino M, Sakai T, Azuma H, Kimura H, Isaka Y, Takahara S, Htinig T, Abe R, Li XK.
  • Journal Title: J Heart Lung Transplant. 27(4) Pages: 362-71
  • Peer Reviewed
• [Journal Article] Foxp3-expressing regulatory T cells expanded with CD28 superagonist antibody can prevent rat cardiac allograft rejection (2008)
  • Author(s): Kitazawa Y, et.al.
  • Journal Title: J Heart & Lung Transplant 27(4) Pages: 362-71
  • Peer Reviewed
• [Presentation] Bone marrow mesenchymal stem cells suppress lymphocyte prollferation and prevent rat graft-versus-host disease but cloned MSCs are failed Categoly (2009)
  • Author(s): 北沢祐介
  • Organizer: 第36回日本臓器保存生物医学会定期学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-04-21
• [Presentation] Bone marrow mesenchymal stem cells suppress lymphocyte proliferation and prevent rat graft-versus-host disease but cloned MSCs are failed Categoly (2009)
  • Author(s): Kitazawa Y
  • Organizer: 第36回日本臓器保存生物医学会定期学術集会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2009-04-21
• [Presentation] superagonlst CD28 antibody e xpand antigen-specific Foxp3-expressing nTreg cells to prevent graft-versus-ho st disease (2008)
  • Author(s): 北沢祐介
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] superagonistCD28抗体で増殖されたFoxP3陽性Treg細胞の抗原特異性について (2008)
  • Author(s): 北沢祐介
  • Organizer: 第35回日本臓器保存生物医学会定期学術集会
  • Place of Presentation: 東京
• [Presentation] superagonist CD28 antibody p referentially expanded functional Foxp3 -expressing nTreg cells to prevents the graft-versus-host disease (2008)
  • Author(s): 北沢祐介
  • Organizer: 国際移植学会
  • Place of Presentation: オーストラリア(シドニー)
• [Presentation] Superagonist CD28 antibody preferentially expanded functional Foxp3-expressing nTreg cells to prevents the graft-versus-host disease (2008)
  • Author(s): 北沢祐介
  • Organizer: 国際移植学会
  • Place of Presentation: オーストラリア(シドニー)
• [Presentation] SuperagonistCD28抗体で増殖されたFoxP3陽性Treg細胞の抗原特異性について (2008)
  • Author(s): 北沢祐介
  • Organizer: 第35回日本臓器保存生物医学会定期学術集会
  • Place of Presentation: 東京
• [Presentation] Superagonist CD28 antibody expand antigen-specific Foxp3-expressing nTreg cells to prevent graft-versus-host disease (2008)
  • Author(s): 北沢祐介
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都