Analysis of signaling pathway for proliferation and survival of lymphatic endothelial cell and application for clinical therapy against lymphangioma

• Project/Area Number: 20790942
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: FUJINO Akihiro National Research Institute for Child Health and Development, 生殖・細胞医療研究部, 共同研究員 (50306726)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: リンパ管腫 / PI3K / リンパ管腫モデル動物 / MAPK / PI3K/AKT / D2-40 / Podoplanin / Proxl抗体 / 試験管内スクリーニング / AKT

Research Abstract

PI3K/AKT signaling pathway is activated in lymphatic endothelial cells in human lymphangioma tissue. Inhibitors for PI3K/AKT were applied to human lymphangioma derived lymphatic endothelial cells (HL-LECs) for apoptosis assay, failing to see any specific reaction in vitro.
Five primary HL-LECs were established and transplanted to immunodeficient NOG mice, resulting in lymphangioma-like cystic tissue formation in all cases.

Research Products

• [Journal Article] リンパ管腫内リンパ液動態の検討 (2011)
  • Author(s): 藤野明浩, 他
  • Journal Title: リンパ学 34(1)(in press)
• [Journal Article] 縦隔疾患 縦隔腫瘍 リンパ管腫 (2009)
  • Author(s): 藤野明浩、森川康英
  • Journal Title: 日本臨床別冊呼吸器症候群III Pages: 531-533
• [Presentation] KINETICS OF LYMPHATIC FLUID IN LYMPHANGIOMA STUDIED BY SCINTIGRAPHY. (2010)
  • Author(s): Fujino A, et al.
  • Organizer: 11th European Congress of Paediatric Surgery
  • Place of Presentation: Bern, Switzerland
• [Presentation] 重症・難治性リンパ管腫の克服を目指して-厚生労働省難治性疾患克服研究事業によるリンパ管腫研究- (2010)
  • Author(s): 藤野明浩、森川康英、上野滋、岩中督
  • Organizer: 日本小児外科学会雑誌46(3):663
• [Presentation] シンチグラフィによるリンパ管腫内リンパ液動態の研究 (2010)
  • Author(s): 藤野明浩、北村正幸, 黒田達夫, 北野良博, 森川信行, 田中秀明, 高安肇, 武藤充, 松田諭, 山根裕介, 正木英一
  • Organizer: 日本小児外科学会雑誌46(3):661
• [Presentation] リンパ管腫内リンパ液動態の研究 (2010)
  • Author(s): 藤野明浩、北村正幸, 黒田達夫, 北野良博, 森川信行, 田中秀明, 高安肇, 武藤充, 松田諭, 山根裕介, 正木英一
  • Organizer: 第34回日本リンパ学会総会
  • Place of Presentation: 東京
• [Presentation] リンパ管腫由来リンパ管内皮細胞の特性 (2010)
  • Author(s): 藤野明浩, 他
  • Organizer: 第7回血管腫・血管奇形研究会
  • Place of Presentation: 松山
• [Presentation] CHARACTERIZATION OF HUMAN LYMPHANGIOMA DERIVED LYMPHATIC ENDOTHELIAL CELLS. (2010)
  • Author(s): Fujino A, et al.
  • Organizer: 23th International Symposium of Pediatric Surgery Research
  • Place of Presentation: Tokyo, Japan
• [Presentation] Kinetics of lymphatic fluid in lymphangioma ; the 2nd report. (2010)
  • Author(s): Fujino A, et al.
  • Organizer: 3rd World Congress of Pediatric Surgery
  • Place of Presentation: Delhi, India
• [Presentation] CHARACTERIZATION OF HUMAN LYMPHANGIOMA DERIVED PHATIC ENDOTHELIAL CELLS. (2009)
  • Author(s): Fujino A
  • Organizer: 56^<th> British Association of Paediatric Surgeons & 10^<th> EUPSA Joint Annual International Congress
  • Place of Presentation: Graz, Austria
  • Year and Date: 2009-06-19
• [Presentation] CHARACTERIZATION OF HUMAN LYMPHANGIOMA DERIVED LYMPHATIC ENDOTHELIAL CELLS (2009)
  • Author(s): Akihiro Fujino
  • Organizer: 56^<th> British Association of Paediatric Surgeons & 10th EUPSA Joint Annual International Congress
  • Place of Presentation: Graz, Austria
  • Year and Date: 2009-06-19
• [Presentation] リンパ管腫由来リンパ管内皮細胞の嚢胞形成特性 (2008)
  • Author(s): 藤野明浩
  • Organizer: 第24回日本小児がん学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2008-11-16
• [Presentation] リンパ管腫由来リンパ管内皮細胞の嚢胞形成特性 (2008)
  • Author(s): 藤野明浩
  • Organizer: 第24回日本小児がん学会
  • Place of Presentation: 幕張
• [Remarks] ホームページ等