Study of Carboxypeptidase M expression profiles in patients with lung cancer

• Project/Area Number: 20790982
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: FUJIWARA Naoyuki 東京医科歯科大学, 大学院・医歯学総合研究科, 医系・助教 (80451912)
• Co-Investigator(Kenkyū-buntansha): AKASHI Takumi 東京医科歯科大学, 医学部附属病院, 医系・准教授 (60242202)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: カルボキシペプチダーゼM / Carboxypeptidase M / CPM / 肺がん / Lung Cancer / 肺胞上皮細胞 / 発がん動物モデル / カルボキシペプチターゼM / EGFR / 肺癌 / 蛋白質 / 病理学

Research Abstract

We investigated the localization of CPM in lung cancer of human and rat. Anti-rat CPM antibody 7F9 was applied to a BHP-induced rat lung cancer model. In immunohistochemistry, CPM was localized in the cell membrane of pulmonary alveolar type II epithelial cells in normal tissue part, and also was in the cell membrane of pulmonary cancer part. In immunohistochemistry for CPM and TTF-1, positive cells were well consistent. Expression level of CPM in western blotting was increased with time. Therefore, CPM was considered one of the cancer-related molecules.

Research Products

• [Presentation] Localization of Carboxypeptidase M in BHP-induced rat lung adenocarcinomas (2009)
  • Author(s): Naoyuki Fujiwara
  • Organizer: 13th World Conference on Lung Cancer
  • Place of Presentation: San Francisco, California, USA
  • Year and Date: 2009-08-01
• [Presentation] ラット肺癌モデルを使用したカルボキシペプチダーゼMの局在の検討 (2009)
  • Author(s): 藤原直之,小島勝雄,荒井裕国,熊谷二朗,畑裕
  • Organizer: 第49回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-12
• [Presentation] Localization ofCarboxypeptidase M in BHP-induced ratlung adenocarcinomas. 13th WorldConference on Lung Cancer (2009)
  • Author(s): Naoyuki Fujiwara, Katsuo Kojima, Hirokuni Arai, Jiro Kumagai, YutakaHata
  • Organizer: San Francisco
  • Place of Presentation: California, USA
• [Presentation] Localization of Carboxypeptidase M in BHP-induced rat lung adenocarcinomas. (2009)
  • Author(s): Naoyuki Fujiwara, Katsuo Kojima, Hirokuni Arai, Jiro Kumagai, Yutaka Hata
  • Organizer: 13th World Conference on Lung Cancer
  • Place of Presentation: San Francisco,California,USA
• [Presentation] 畑裕ラット肺癌モデルを使用したカルボキシペプチダーゼMの局在の検討 (2009)
  • Author(s): 藤原直之、小島勝雄、荒井裕国、熊谷二朗
  • Organizer: 第49回日本呼吸器学会学術講演会
  • Place of Presentation: 東京