Effect of bisphosphonate on osteoblast differentiation of mesenchymal stem cells

• Project/Area Number: 20791042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: FUJITA Hirofumi Okayama University, 大学院・医歯薬学総合研究科, 助教 (20423288)
• Research Collaborator: KUROKAWA Kaduko 岡山大学, 大学院・医歯薬学総合研究科, 大学院生
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ビスフォスフォネート / 骨粗鬆症 / 骨芽細胞 / 間葉系幹細胞 / 分化 / 石灰化 / 骨シアロタンパク質

Research Abstract

Our results indicated that bisphoshonate suppressed osteoblast differentiation and suggested that the anti-bone resorptive effect of bisphoshonate is not involved in RANKL expression of MSCs-derived osteoblast. We also found that GGOH strongly suppressed the bisphoshonate-induced apoptosis in MSCs.

Research Products

• [Journal Article] α-Tocopheryl succinate induces rapid and reversible phosphatidylserine externalization in histiocytic lymphoma through the caspase-independent pathway (2010)
  • Author(s): Fujita H, et al
  • Journal Title: Mol Cell Biochem 333 Pages: 137-149
  • Peer Reviewed
• [Journal Article] α-Tocopheryl scnate induces rap id and reversible phosphatidylserine externalization in histiocytic lymphoma through the caspase-independent pathway (2010)
  • Author(s): Fulita H, et.al
  • Journal Title: Mol Cell Biochem 333 Pages: 137149-137149
  • Peer Reviewed
• [Journal Article] Mechanism of cell death by 5-aminolevulinic acid-based photodynamic action and its enhancement by ferrochelatase inhibitors in human histiocytic lymphoma cell line U937 (2009)
  • Author(s): Amo T, Fujita H, et al
  • Journal Title: Cell Biochem Funct 27(8) Pages: 503-15
  • Peer Reviewed
• [Journal Article] Regulation of 5-aminolevulinic Acid-mediated Protoporphyrin IX-accumulation in Human Urothelial Carninnmas. (2009)
  • Author(s): Inoue K.Fuiita H, et.al
  • Journal Title: Pathobiology 76 Pages: 303314-303314
  • Peer Reviewed
• [Journal Article] Mechanism of cell death by 5-aminolevulinic acid-based photodynamic action and its enhancement by ferrochelatase inhibitors in human histiocytic lymphoma cell line U937. (2009)
  • Author(s): Amo T, Fuiita H, et.al
  • Journal Title: Cell Biochem Fount 27 Pages: 503515-503515
  • Peer Reviewed
• [Journal Article] Alpha-lipoic acid suppresses 6-hydroxydopamine- induced ROS generation and apoptosis through the stimulation of glutathione synthesis but not by the expression of heme oxygenase-1 (2008)
  • Author(s): H Fujita, et al
  • Journal Title: Brain research 1206 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Alpha-lipoic acid suppresses 6-hydroxydopamine-induced ROS generation and apoptosis through the stimulation of glutathione synthesis but not by the expression of heme oxygenase-1 (2008)
  • Author(s): Hirofumi Fujita, et.al.
  • Journal Title: Brain research 1206 Pages: 1-12
  • Peer Reviewed
• [Journal Article] L-carnitine suppresses oleic acid-induced membrane permeability transition of mitochondria (2008)
  • Author(s): Oyanagi E, Fujita H, et.al.
  • Journal Title: Cell Biochem Funct 26 Pages: 778-786
  • Peer Reviewed
• [Journal Article] Mechanism of 3-nitropropionic acid-induced membrane permeability transition of isolated mitochondria and its suppression by L-carnitine (2008)
  • Author(s): Nishimura M, Fujita H, et.al.
  • Journal Title: Cell Biochem Funct 26 Pages: 881-891
  • Peer Reviewed
• [Presentation] 間葉系幹細胞における骨粗鬆症治療薬リセドロネートの骨芽細胞分化とRANKL発現への影響 (2010)
  • Author(s): 藤田洋史, ら
  • Organizer: 第115回日本解剖学会総会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 間葉系幹細胞における骨粗懸症治療薬リセドロネートの骨芽細胞分化とRANKL発規への影響 (2010)
  • Author(s): 藤田洋史, ら
  • Organizer: 第115回日本解剖学会総会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 骨粗鬆症治療薬リセドロネートの間葉系幹細胞に対する作用-骨芽細胞分化を中心として- (2009)
  • Author(s): 黒川佳津子, 藤田洋史, ら
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] 間葉系幹細胞の骨芽細胞分化とそれに対するリセドロネートの作用 (2009)
  • Author(s): 黒川佳津子, 藤田洋史, ら
  • Organizer: 第50回日本生化学会中国・四国支部例会
  • Place of Presentation: 鳥取
• [Presentation] 骨粗髪症治療薬リセドロネートの間葉系幹細胞に対する作用-骨芽細胞分化を甲心として- (2009)
  • Author(s): 黒川佳津子, 藤田洋史, ら
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
• [Presentation] 間葉系幹細胞の骨芽細胞分化に対するリセドロネートの影響 (2008)
  • Author(s): 藤田洋史, 黒川佳津子, ら
  • Organizer: 第5回ビスフォスフォネートUpdate
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-29
• [Presentation] 間葉系幹細胞の骨芽細胞分化に対するリセドロネートの影響 (2008)
  • Author(s): 藤田洋史, 他5名
  • Organizer: 日本骨代謝学会(第5回ビスフォスフォネートUpdate)
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-29
• [Presentation] Effect of nitrogen containing bisphosphonate on osteoblast differentiation of rat mesenchymal stem cell (2008)
  • Author(s): 黒川佳津子、藤田洋史, ら
  • Organizer: BMB2008(第81回日本生化学会大会)
  • Place of Presentation: 神戸
• [Presentation] Effect of nitrogen containing bisphosphonate on osteoblast differentiation of rat mesenchymal stem cell (2008)
  • Author(s): 黒川佳津子、藤田洋史, 他4名
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸