Catecholamines accelerate BMP signaling ; the exercise enhance bone formation

• Project/Area Number: 20791048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Osaka City University
• Principal Investigator: HASHIMOTO Yusuke Osaka City University, 大学院・医学研究科, 講師 (10382178)
• Research Collaborator: OHTA Yoichi 大阪市立大学, 大学院・医学研究科, 前期研究医 ; UEMURA Takuya 大阪市立大学, 大学院・医学研究科, 大学院生
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨形成蛋白(Bone morphogenetic protein ; BMP) / 運動 / カテコラミン / 骨形成蛋白(BMP) / PLA-DX-PEG polymer / PLA-DX-PEG polmer

Research Abstract

Topical effects of a catecholamine on bone morphogenetic protein (BMP)-induced ectopic bone formation were investigated in both in vivo and in vitro experimental systems. The mass of ossicles ectopically induced by BMP-2 was increased by the addition of catecholamine into a biodegradable BMP-2 carrier, in a dose-dependent manner. The expression level of alkaline phosphatase (ALP) in ST2 cells was consistently elevated by BMP-2 and was further elevated by the addition of catecholamine. Catecholamines might play a part in the regulation of bone metabolism in exercise and sports activities through elevation of the plasma level of catecholamine.

Research Products

• [Journal Article] Antitumor necrotic factor agent promotes BMP-2-induced ectopic bone formation (2010)
  • Author(s): Eguchi Y, Wakitani S, Imai Y, Naka Y, Hashimoto Y, Nakamura H, Takaoka K
  • Journal Title: J Bone Miner Metab 28(2) Pages: 157-64
  • NAID: 10027207128
  • Peer Reviewed
• [Journal Article] Highly sensitive ELISA for determining serum keratan sulphate levels in the diagnosis of OA (2010)
  • Author(s): Wakitani S, Okabe T, Kawaguchi A, Nawata M, Hashimoto Y
  • Journal Title: Rheumatology (Oxford) 49(1) Pages: 57-62
  • Peer Reviewed
• [Journal Article] In vivo comparison of wear particles between highly crosslinked polyethylene and conventional polyethylene in the same design of total knee arthroplasties (2009)
  • Author(s): Iwakiri K, Minoda Y, Kobayashi A, Sugama R, Iwaki H, Inori F, Hashimoto Y, Ohashi H, Ohta Y, Fukunaga K, Takaoka K.
  • Journal Title: J Biomed Mater Res B Appl Biomater 27;91B(2) Pages: 799-804
  • Peer Reviewed
• [Journal Article] The incidence of the patellar clunk syndrome in a recently designed mobile-bearing posteriorly stabilised total knee replacement (2009)
  • Author(s): Fukunaga K, Kobayashi A, Minoda Y, Iwaki Y, Hashimoto Y, Takaoka K.
  • Journal Title: J Bone Joint Surg Br 91-B Pages: 463-8
  • Peer Reviewed
• [Journal Article] RNA interference for noggin enhances the biological activity of bone morphogenetic proteins in vivo and in vitro (2009)
  • Author(s): Takayama K, Suzuki A, Manaka T, Taguchi S, Hashimoto Y, Imai Y, Wakitani S, Takaoka K
  • Journal Title: J Bone Miner Metab 27(4) Pages: 402-11
  • NAID: 10027204830
  • Peer Reviewed
• [Journal Article] Repair of long intercalated rib defects in dogs using recombinant human bone morphogenetic protein-2 delivered by a synthetic polymer and beta-tricalcium phosphate (2009)
  • Author(s): Hoshino M, Egi T, Terai H, Namikawa T, Kato M, Hashimoto Y, Takaoka K.
  • Journal Title: J Biomed Mater Res A. 90(2) Pages: 514-21
  • Peer Reviewed
• [Journal Article] The incidence of the patellar clunk syndrome in a recently designed mobile-bearing posteriorly stabilised total knee replacement. (2009)
  • Author(s): Fukunaga K, et al.
  • Journal Title: J Bone Joint Surg Br 91 Pages: 463-468
  • Peer Reviewed
• [Journal Article] In vivo comparison of wear particles between highly crosslinked polyethylene and conventional polyethylene in the same design of total knee arthroplasties. (2009)
  • Author(s): Iwakiri K, et al.
  • Journal Title: J Biomed Mater Res B Appl Biomater. 91 Pages: 799-804
  • Peer Reviewed
• [Journal Article] Antitumor necrotic factor agent promotes BMP-2-induced ectopic bone formation. (2009)
  • Author(s): Eguchi Y, et al.
  • Journal Title: J Bone Miner Metab. 28 Pages: 157-164
  • Peer Reviewed
• [Journal Article] Skiing-induced rupture of the extensor pollicis longus tendon: a report of three cases (2008)
  • Author(s): Uemura T, Kazuki K, Hashimoto Y, Takaoka K
  • Journal Title: Clin J Sport Med. 18(3) Pages: 292-4
  • Peer Reviewed
• [Journal Article] Expression profiles of phosphodiesterase 4D splicing variants in osteoblastic cells (2008)
  • Author(s): Nomura-Furuwatari C, Wakitani S, Hashimoto Y, Imai Y, Ohta Y, Nakagawa K, Nakao Y, Takayama K, Manaka T, Takaoka K
  • Journal Title: J Bone Miner Metab 26 Pages: 152-158
  • NAID: 10024126436
  • Peer Reviewed
• [Journal Article] Bilateral osteochondritis dissecans of the lateral femoral condyle following bilateral total removal of lateral discoid meniscus: a case report (2008)
  • Author(s): Hashimoto Y, Yoshida G, Tomihara T, Matsuura T, Satake S, Kaneda K, Shimada N
  • Journal Title: Arch Orthop Trauma Surg 128(11) Pages: 1265-8
  • Peer Reviewed
• [Journal Article] Repair of long intercalated rib defects in dogs using recombinant human bone morphogenetic protein-2 delivered by a synthetic polymer and beta-tricalcium phosphate (2008)
  • Author(s): Hoshino M, et.al.
  • Journal Title: J Biomed Mater Res A (In press)
  • Peer Reviewed
• [Journal Article] Skiing-induced rupture of the extensor pollicis longus tendon : a report of three cases (2008)
  • Author(s): Uemura T, et.al.
  • Journal Title: Clin J Sport Med 18 Pages: 292-294
  • Peer Reviewed
• [Journal Article] Expression profiles of phosphodiesterase 4D splicing variants in osteobla stic cells (2008)
  • Author(s): Nomura-Furuwatari C
  • Journal Title: J Bone Miner Metab 26 Pages: 152-158
  • Peer Reviewed
• [Presentation] エピネフリンによるBMPの効果促進 (2009)
  • Author(s): 上村卓也, 他
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-11-05
• [Presentation] (2009)
  • Author(s): Hashimoto Y, Tomihara H, Yamazaki S, Takigami J, Nishikino S, Takaoka K.
  • Organizer: 7th biennial ISAKOS congress Anatomical and Computed Tomographic analysis of Femoral Attachment of Anterior Cruciate Ligament
  • Year and Date: 2009-04-05
• [Presentation] (2009)
  • Author(s): Nishikino S, Hashimoto Y, Hara Y, Minoda Y, Iwaki H, Nakamura H.
  • Organizer: The 55th ORS Anatomical and Computed Tomographic analysis of the Femoral Attachment of the Posterior Cruciate Ligament
  • Year and Date: 2009-02-22
• [Presentation] (2009)
  • Author(s): Naka Y, Hashimoto Y, Takaoka K
  • Organizer: The 55th ORS 2009 Meniscus reconstruction by a tendon autograft with recombinant human bone morphogenetic protein-2 in a rabbit model
  • Year and Date: 2009-02-22
• [Presentation] (2009)
  • Author(s): Hashimoto Y, Naka Y, Fukunaga K, Takaoka K
  • Organizer: The55th ORS 2009 Generation of Bone-Tendon-Bone graft using rhBMP and application to the ACL Reconstruction.
  • Year and Date: 2009-02-22
• [Presentation] (2008)
  • Author(s): Hashimoto Y, Tomihara T, Yano K, Iwaki H, Takaoka K.
  • Organizer: The 54th ORS 2008 Anatomical and Computed Tomographic analysis of Femoral Attachment of Anterior Cruciate Ligament
  • Year and Date: 2008-03-02
• [Presentation] Catecholamines accelerate BMP- induced osteoblastic differentiation and bone formation (2008)
  • Author(s): Uemura T, Ohta Y, Nakao Y, H shimoto Y, Takaoka K
  • Organizer: The American Society for Bone and Mineral Research(30th)
  • Place of Presentation: モントリオール、カナダ
• [Presentation] カテコラミンはBMPによる骨芽細胞分化、骨形成作用促進作用を増強する (2008)
  • Author(s): 上村卓也, 他
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] カテコラミンはBMPによる骨芽細胞分化、骨形成作用促進作用を増強する (2008)
  • Author(s): 上村卓也, 他
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪