Analysis of cytokine secretion and its application using macrophage specific syntaxin 3 knockout mice

• Project/Area Number: 20791063
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Gunma University
• Principal Investigator: IRIUCHIJIMA Nobuhisa Gunma University, 医学部, 助教 (10431719)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: シンタキシン3 / ノックアウトマウス / syntaxin3 / TNFα / コンディショナルノックアウト

Research Abstract

Using macrophage specific syntaxin 3 knockout mice, whether syntaxin 3 was involved in the secretion of TNFα, it was evaluated by staining and ELISA. Unlike the initial estimates,the syntaxin 3 was not found to be involved in the secretion of TNFα. However, syntaxin 3 to move from the Golgi apparatus to the cell periphery, It was thought it is involved in the secretion of other cytokines.