Analysis for control systems of heme oxygenase-1 in human villus cells and development for immunohistochemical staining of heme oxygenase-1 in human placenta

• Project/Area Number: 20791160
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Jichi Medical University
• Principal Investigator: SUZUKI Hirotada Jichi Medical University, 医学部, 助教 (90406116)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 産科学 / 医療・福祉 / 妊娠高血圧腎症 / sFlt-1 / sEndoglin / HO-1

Research Abstract

We cultured primary human trophoblast or BeWo cell with ZuPP (heme oxygenase-1 inhibitor) and analized the mRNA levels of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) by RT-RCR. These mRNA levels did not change.
We are now collecting human placenta tissues. In future, we will try immunohistochemical staining of heme oxygenase-1 in these placenta tissues.