The mechanism of water absorption in the endolymphatic sac

• Project/Area Number: 20791179
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: NAKAYA Kazuhiro Tohoku University, 病院, 助教 (60466563)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: RNAi / 内リンパ水腫

Research Abstract

AQP2 mRNA was knocked down by placing siRNA specific to rat AQP2 at the round window membrane. The samples were harvested 48 hours after the intervention. The immunohistochemical study under the fluorescent microscope revealed that the expression of the AQP2 in the endolymphatic sac was reduced in the group treated with AQP2 siRNA compared to the group treated with negative control siRNA. Neither endolymphatic hydrops nor endolymphatic collapse occurred in both groups.