Effects to inhibit innate immunity of fungi, especially Alternaria-Experiments using dendritic cell-

• Project/Area Number: 20791231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: WADA Kota Jikei University School of Medicine, 医学部, 助教 (20307482)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Alternaria / 樹状細胞 / 線維芽細胞 / 自然免疫

Research Abstract

Alternaria alternate extract induced IL-6 and IL-8 production from nasal fibroblasts in dose and time dependent manner. Alternaria alternate which treated at 56, 100℃ did not induce IL-6 and IL-8. It is suggesting that these production occurred by a heat-labile protein(s) or glycoprotein(s). From inhibition assay, Asparte protease-like product, not serine protease, is one of the products of alternaria alternate for these reactions. P38 MAP kinase plays an important role for IL-6 and IL-8 production by alternaria alternate from nasal fibroblasts. We were not able to examine the experiment of dendritic cells. We assume it the problem of the study after the next year.