Analysis of the molecular mechanism for human pigment epithelium-derived factor (hPEDF) mediated photoreceptor cell neuroprotection
| Project/Area Number |
20791259
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kyushu University |
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Principal Investigator |
IKEDA Yasuhiro Kyushu University, 大学病院, 助教 (20380389)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 眼科学 / 網膜色素変性 / 神経保護 / 遺伝子治療 / 視細胞保護 / ヒト色素上皮由来因子 / サル由来レンチウイルスベクター / アポトーシス誘導因子 / 網膜色素変 / ヒト色素上皮由来因子(hPEDF) / サル由来レンチウイルス(SIV)ベクター / アポトーシス誘導因子(AIF) |
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| Research Abstract |
The key observations made in this study are as follows: 1) PEDF efficiently rescued apoptosis induced by serum starvation in R28 cells derived from rat retina ; 2) AIF but not caspases was a key effector of the serum starvation-induced apoptosis, and PEDF prevented AIF from translocating into the nucleus after serum starvation; 3) Nuclear translocation of AIF was also observed in apoptotic photoreceptors of RCS rats, and was significantly inhibited by retinal gene transfer of PEDF in vivo, resulting in a substantial delay in retinal degeneration; and 4) PEDF prevented the AIF release through upregulation of Bcl-2.
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Report
(3 results)
Research Products
(3 results)
