| Project/Area Number |
20791272
|
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| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 眼発生 / 再生医学 / 移植・再生医療 / Dlkタンパク / Ptnタンパク / 角膜上皮細胞 |
|---|
| Research Abstract |
Fibroblasts, especially mouse cell line 3T3, support clonal growth of epithelial cells via direct cell-cell contact. We compared gene expression profile between 3T3 and less-colony supportive corneal fibroblasts, and found abundant expression of Dlk1 in 3T3. However, overexpression or inhibition of Dlk1 in feeder cells did not affect on the epithelial colony supporting ability. Although Dlk1 is highly expressed in 3T3, Dlk1 may not be related to the feeder layer activity.
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